Intestinal Effects of Brewers’ Spent Grain Extract In Ovo (Gallus gallus)—A Pilot Study

Animals, Год журнала: 2025, Номер 15(3), С. 303 - 303

Опубликована: Янв. 22, 2025

Upcycling brewers’ spent grain (BSG) into poultry feed needs to be optimized. Since broiler chickens inefficiently digest fiber, we created a water-soluble BSG extract (BSGE) explore this fraction’s potential nutritional benefits. We utilized intra-amniotic administration (in ovo) target the gastrointestinal tract of embryos. BSGE increased villus surface area and goblet cell quantity size, implying improved duodenal development. The also changed cecal Escherichia coli (E. coli) Clostridium abundances. Synchrotron X-ray fluorescence microscopy, along with zinc iron transporter relative expression, did not reveal significant changes by BSGE. These findings highlight for functional component, underscoring value upcycling byproduct. This pilot study supports future work exploring impact within its effects over long-term consumption.

Язык: Английский

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Understanding the molecular bridges between the drugs and immune cell

Pharmacology & Therapeutics, Год журнала: 2025, Номер 267, С. 108805 - 108805

Опубликована: Фев. 4, 2025

Язык: Английский

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Desmodium styracifolium: Botanical and ethnopharmacological insights, phytochemical investigations, and prospects in pharmacology and pharmacotherapy

Heliyon, Год журнала: 2024, Номер 10(3), С. e25058 - e25058

Опубликована: Янв. 21, 2024

The purpose of this inquiry is to provide a conprehensive summary and analysis the literature concerning pharmacological properties components that can be extracted from Desmodium styracifolium, preparation in Chinese medicine. This study also aims explore their potential application elaborating medicinal products for effective prevention treatment such conditions as urolithiasis, cholelithiasis, type 2 diabetes mellitus, metabolic syndrome, pro-oxidant inflammatory processes, etc.Several experimental studies confirmed D. styracifolium influence mineral metabolism, decrease concentration constituents involved formation urinary calculi, reduce encrustation tract, well alleviate damage caused by crystal structures. beneficial impact achieved through combination antioxidant anti-inflammatory actions, along with urine alkalinization. cholelitholytic, choleretic, hepatoprotective effects plants have been confirmed, primarily ascribed activation hepatic Xα receptor bile acid receptor, farnesoid X flavonoid shaftoside.Special attention focused on therapeutic applications flavonoids derived diseases associated development chronic inflammation systemic response, emphasizing ability exert acting directly modulation transcription factors.It concluded new strategies acute processes may rely promising dosage forms subsequent preclinical clinical trials.

Язык: Английский

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Efficacy of various regimens of bioactive marine fish concentrate, glucosamine sulfate, and chondroitin sulfate in treating knee osteoarthritis

PAIN JOINTS SPINE, Год журнала: 2025, Номер 14(4), С. 193 - 198

Опубликована: Янв. 2, 2025

Background. Osteoarthritis (OA) is one of the most common joint diseases worldwide. The search for effective treatment regimens to reduce symptoms and improve function essential. purpose was compare effectiveness different bioactive marine fish concentrate (BMFC) in knee OA with oral glucosamine sulfate (GS) chondroitin (CS). Materials methods. study involved 60 patients stage II OA. were divided into three groups: first group received 1 ml BMFC daily (20 injections), second 2 every other day (10 third GS CS tablet form. Clinical efficacy assessed using WOMAC scale on days 3, 7, 14, 21. Results. In that daily, pain decreased by 41.4 % 7 58.7 Joint stiffness 51.9 %, range motion increased 51.3 %. receiving day, reduction 37.1 55.7 49.2 improved 46.0 end treatment. Both groups demonstrated similar efficacy. Patients showed less pronounced improvements. Conclusions. Injectable higher treating compared CS. ­BMFC administration (daily day) proved reducing pain, alleviating stiffness, improving functional capacity. every-other-day regimen achieved comparable fewer injections, enhancing convenience adherence therapy.

Язык: Английский

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Unraveling the parahormetic mechanism underlying the health-protecting effects of grapeseed procyanidins

Redox Biology, Год журнала: 2023, Номер 69, С. 102981 - 102981

Опубликована: Дек. 7, 2023

Proanthocyanidins (PACs), the predominant constituents within Grape Seed Extract (GSE), are intricate compounds composed of interconnected flavan-3-ol units. Renowned for their health-affirming properties, PACs offer a shield against spectrum inflammation associated diseases, such as diabetes, obesity, degenerations and possibly cancer. While monomeric dimeric undergo some absorption gastrointestinal tract, larger oligomeric polymeric counterparts not bioavailable. However, higher molecular weight engage with colonic microbiota, fostering production bioavailable metabolites that metabolic processes, culminating in emergence bioactive agents capable modulating physiological processes. Within this investigation, GSE enriched was employed to explore detail impact. Through comprehensive analysis, present study unequivocally verified gastrointestinal-mediated transformation medium high PACs, thereby establishing bioaccessibility principal catabolite termed 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone (VL). Notably, our findings, encompassing cell biology, chemistry proteomics, converge proposal notion capacity VL activate, upon oxidation corresponding quinone, nuclear factor E2-related 2 (Nrf2) pathway—an process incites cellular defenses mitigates stress-induced responses, challenge brought by TNFα. This mechanistic paradigm seamlessly aligns concept para-hormesis, ultimately orchestrating resilience stress preservation redox equilibrium homeostasis benchmarks health.

Язык: Английский

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Antioxidant and Wound Healing Bioactive Potential of Extracts Obtained from Bark and Needles of Softwood Species

Antioxidants, Год журнала: 2023, Номер 12(7), С. 1383 - 1383

Опубликована: Июль 4, 2023

Interest in the extraction of phytochemical bioactive compounds, especially polyphenols from biomass, has recently increased due to their valuable biological potential as natural sources antioxidants, which could be used a wide range applications, foods and pharmaceuticals green polymers bio-based materials. The present research study aimed provide comprehensive chemical characterization composition forest biomass (bark needles) softwood species (Picea abies L., H. Karst., Abies alba Mill.) investigate vitro antioxidant antimicrobial activities assess treating healing infected chronic wounds. DPPH radical-scavenging method P–LD were for mechanistic explanation biomolecular effects investigated compounds. (+)-Catechin, epicatechin, rutin, myricetin, 4 hydroxybenzoic p-cumaric acids, kaempherol, apigenin main quantified coniferous (in quantities around 100 µg/g). Also, numerous phenolic flavonoids, stilbenes, terpenes, lignans, secoiridoids, indanes with antioxidant, antimicrobial, anti-inflammatory, antihemolytic, anti-carcinogenic identified. needle extract was more toxic microbial strains than eukaryotic cells that its active wound principles. In this context, developing industrial upscaling strategies is imperative long-term success biorefineries incorporating them part circular bio-economy.

Язык: Английский

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Molecular mechanisms and potential applications of chondroitin sulphate in managing post-traumatic osteoarthritis

Reumatologia/Rheumatology, Год журнала: 2023, Номер 61(5), С. 395 - 407

Опубликована: Окт. 31, 2023

Post-traumatic osteoarthritis (PTOA), a disorder of the synovium, subchondral bone, and cartilage that affects entire joint, constitutes approximately 12% all cases symptomatic osteoarthritis. This review summarizes pathogenetic mechanisms underlie positive influence chondroitin sulphates (CSs) on PTOA as means preventive therapeutic treatment. Mechanisms development involve chondrocytes undergoing various forms cell death (apoptosis, pyroptosis, necroptosis, ferroptosis and/or necrosis). Chondroitin are class glycosaminoglycans improve structure function which is associated with their ability to decrease activation NF-κB p38 MAPK, up-regulate Nrf2. Standardized small fish extract (SSFE) an example drugs can attenuate NF-κB-mediated systemic inflammation, potentially helping reduce joint inflammation degradation, function, alleviate pain disability in patients these conditions.

Язык: Английский

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FORMATION OF REACTIVE OXYGEN AND NITROGEN SPECIES IN RAT LIVER TISSUES UNDER CONDITIONS OF SURGICAL TRAUMA REPRODUCED ON THE BACKGROUND OF EXPERIMENTAL MODEL OF POST-TRAUMATIC STRESS DISORDER

Актуальні проблеми сучасної медицини Вісник Української медичної стоматологічної академії, Год журнала: 2023, Номер 23(3), С. 137 - 142

Опубликована: Ноя. 3, 2023

In an experiment on 42 white rats, the mechanisms of oxidative-nitrosative stress in liver tissues under conditions surgical trauma reproduced background post-traumatic disorder were studied. It has been shown that reproduction experimental model posttraumatic (single prolonged stress) causes development rat tissues: it increases rate superoxide anion radical generation by microsomes, mitochondria and leukocyte NADPH oxidase, NO-synthase activity activating inducible isoform, reduces conjugation constitutive NO-synthases, which is accompanied increase concentration peroxynitrites alkaline alkaline-earth metals. On 7th day after laparotomy against disorder, indicators tissues, such as production microsomes oxidase total metals, significantly exceed their values a single sham operation simulated stress.

Язык: Английский

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EFFECT OF MELATONIN AND QUERCETIN ON INFLAMMATION AND METABOLISM UNDER CONDITIONS OF ROUND-THECLOCK LIGHTING AND HIGH-CALORIE CARBOHYDRATE-LIPID DIET

Fìzìologìčnij žurnal, Год журнала: 2024, Номер 70(1), С. 43 - 51

Опубликована: Янв. 1, 2024

The study is aimed at investigating the impact of exogenous melatonin and quercetin on indices systemic inflammatory response indicators carbohydrate lipid metabolism in blood serum male rats exposed to round-the-clock lighting (RCL) with 1500 lx intensity during last 30 days being kept a 60 day high-calorie carbohydrate-lipid diet (HCCLD, 20% fructose solution proper chow). has demonstrated that restoration concentration by its administration RCL exposure keeping them HCCLD did not fully correct such as content tumor necrosis factor α (TNF-α) C-reactive protein (CRP), well insulin, highdensity lipoprotein (HDL), very low-density (VLDL) triglycerides, HOMA-IR insulin resistance index. under experimental conditions was accompanied significant increase (by 85.9%), decrease TNF-α 53.9%), CRP 54.4%), glucose 49.2%), 49.6%), VLDL 49, 2%) triglycerides 49.3%), an HDL twofold), but these (with exception index, which decreased 62.4%) reach values intact group. combined effect HLLD significantly improved response, confirmed more levels TNF-α, CRP, concentration, index compared separate use quercetin.

Язык: Английский

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The influence of monosodium glutamate on the development of oxidative-nitrosative stress in the large cerebral hemispheres of rats under the combination of a change in the “light-dark” cycle and a systemic inflammatory response

Modern medical technology, Год журнала: 2024, Номер 16(2), С. 115 - 121

Опубликована: Июнь 18, 2024

Today, the issue of consequences disrupting normal “light-dark” cycle is becoming increasingly important, and link between development a systemic inflammatory response (SIR) circadian rhythm disorders has been confirmed. The effect monosodium glutamate on human body being actively studied. aim work was to find out influence oxidative-nitrosative stress in homogenate large cerebral hemispheres rats with combination acute desynchronosis (AD), SIR administration glutamate. Material methods. research carried 72 white Wistar weighing 150–200 different sexes, divided into 5 groups: control (n = 15), AD 13), 14), SIR, AD, 15). To simulate (12 hours light, 12 darkness) formed for 3 weeks, next days phases were shifted back by 6 hours. reproduced intraperitoneal injection Salmonella typhi lipopolysaccharide first week at dose 0.4 μg/kg times per week, following seven weeks – once week. Sodium administered intragastrically 20 30 mg/kg, dissolved 0.5 ml distilled water. In 10 % hemispheres, we determined rate superoxide anion radical (SAR) production, content products that react thiobarbituric acid (TBA-reactants), their increase, activity catalase, dismutase (SOD), ornithine decarboxylase (ODC), concentration nitrites, peroxynitrites, total NO-synthase (NOS), its constitutive (cNOS) inducible (iNOS) isoforms. Results. group combination, compared control, noted: an increase basic production SAR 175.8 %, microsomal pathway 20.0 mitochondrial 51.2 TBA-reactants 83.4 61.7 decrease SOD 57.1 catalase 38.1 %; peroxynitrite 116.7 ODC 161.5 NOS 25.6 iNOS 27.4 cNOS 15.0 nitrite 35.0 brain tissues. Conclusions. leads increased stress, inhibition antioxidant protection; contributes activation general iNOS, suppresses cNOS.

Язык: Английский

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