Plant Cell Reports, Год журнала: 2024, Номер 43(5)
Опубликована: Май 1, 2024
Язык: Английский
Horticultural Plant Journal, Год журнала: 2024, Номер unknown
Опубликована: Март 1, 2024
The CRISPR/Cas9 genome-editing system serves as a pivotal tool for enhancing crop genetics. Within this system, single guide RNAs (sgRNAs) are instrumental in the precise cleavage of DNA double strands. However, efficiency gene editing varies among sgRNAs, emphasizing need to meticulously select target sites, especially context Citrullus lanatus, species notorious its challenging and inefficient generation transgenic plants through stable transformation. This study employed an Agrobacterium rhizogenes-mediated hairy root method assess effective sites ClCIPK17 across various species. Hairy roots were successfully induced different plant tissues at diverse growth stages mucosospermus, amarus. Employing vector with two sgRNAs (sgRNA1 sgRNA5) positioned within conserved regions exon 1 5 targeted mutations detected 90.9% accessions four Notably, 73.94% all examined exhibited sgRNA1 site, while sgRNA5 site showed no mutations. Among 31 mutation types identified base deletion was most prevalent. Using ClCIPK17, watermelon buds obtained from explants, confirmed. These findings underscore viability transformation assessing sgRNA targets
Язык: Английский
Процитировано
8
Horticultural Plant Journal, Год журнала: 2024, Номер unknown
Опубликована: Март 1, 2024
Photosynthesis is one the most important chemical reaction in plants, and it ultimate energy source of any living organisms. The light dark reactions are two essential phases photosynthesis. Light harvests to synthesize ATP NADPH through an electron transport chain, as well giving out O2; fixes CO2 into six carbon sugars by utilizing from ATP. Subsequently, plants convert optical for maintaining growth development absorbing energy. Here, firstly, we highlighted biological importance photosynthesis, hormones metabolites, photosynthetic regulating enzymes, signaling components that collectively regulate photosynthesis tomato. Next, reviewed advances tomato including aspects genetic basis improvement. Numerous genes gradually uncovered, interaction network among those remains be constructed. Finally, occurring fruit relationship between leaf were discussed. Leaves fruits photosynthate sources sinks respectively, exists. Additionally, future perspectives needs addressed on proposed.
Язык: Английский
Процитировано
7
Agriculture, Год журнала: 2024, Номер 14(1), С. 90 - 90
Опубликована: Янв. 1, 2024
The availability of genome-sequencing and genome-editing techniques has increased the applicability innovative solutions, opening up revolutionary prospects for developments in horticultural plant breeding. Cucurbitaceae family is a group plants great importance horticulture due to their high nutritional economic value. These serve as important models elucidating principles development refining yield improvement strategies. While traditional breeding approaches have made significant contributions production cucurbits, they also been limited by reduced genetic diversity lower rates variation inherent these species. This comprehensive review summarises latest genome editing cucurbits. It covers various aspects enhancing traits resist biotic stresses such pathogenic fungi viruses, well abiotic adverse climate change, especially caused drought salinity. study focused on improvements quality optimisation architecture, sex determination flowers fruit features. provides insights that may hold promise future crop serves an reference advancement gene-editing technologies
Язык: Английский
Процитировано
6
Frontiers in Plant Science, Год журнала: 2024, Номер 15
Опубликована: Март 26, 2024
Citrus canker disease affects citrus production. This is caused by Xanthomonas citri subsp. (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), translocated from the pathogen to host plant cells. PthA4 binds binding elements (EBEs) in promoter region of susceptibility gene LOB1 (EBE -LOBP) activate its expression and subsequently cause symptoms. Previously, Cas12a/CBE co-editing method was employed disrupt EBE -LOBP pummelo, which highly homozygous. However, most commercial cultivars are heterozygous hybrids more difficult generate homozygous/biallelic mutants. Here, we edit Hamlin ( sinensis ), hybrid cultivar grown worldwide. Binary vector GFP-p1380N-ttLbCas12a:LOBP1-mPBE:ALS2:ALS1 constructed shown be functional via Xcc-facilitated agroinfiltration leaves. construct allows selection transgene-free regenerants GFP, edits ALS chlorsulfuron-resistant as marker for genome editing resulting transient T-DNA nCas9-mPBE:ALS2:ALS1, gene(s) interest (i.e., this study) through ttLbCas12a, thus creating citrus. Totally, 77 plantlets were produced. Among them, 8 transgenic plants (#Ham GFP 1 - #Ham 8), 4 NoGFP 4), rest wild type. plantlets, three lines 1, 2 3) contained biallelic mutations pthA4 , one line 4) had homozygous . We achieved 5.2% mutation efficiency –LOBP C. cv. Hamlin, compared 1.9% pummelo previous study. Importantly, four 3 survived resistant against canker. Taken together, has been successfully used canker‐resistant T0 generation biallelic/homozygous LOB1.
Язык: Английский
Процитировано
6
PLANT PHYSIOLOGY, Год журнала: 2024, Номер 195(3), С. 1880 - 1892
Опубликована: Март 13, 2024
Abstract Manipulation of gene expression is central to understanding function, engineering cell behavior, and altering biological traits according production demands. Nuclease-dead Cas9 (dCas9), a variant active Cas9, offers versatile platform for the precise control genome function without DNA cleavage. Notably, however, an effective universal dCas9-based transcriptional repression system remains unavailable in plants. The noncanonical histone acetyltransferase TENDRIL-LESS (CsTEN) responsible chromatin loosening modification cucumber (Cucumis sativus). In this study, we engineered regulation tool by fusing TEN its truncated proteins with dCas9. full-length dCas9-TEN protein substantially repressed expression, N-terminal domain identified as core domain. We subsequently validated specificity efficacy through both transient infection genetic transformation Arabidopsis (Arabidopsis thaliana). electrophoretic mobility shift assay (EMSA) revealed ability bind chromatin, which may promote target binding dCas9 complex enhance effect. Our enriches arsenal tools available precision breeding crops.
Язык: Английский
Процитировано
4
Horticultural Plant Journal, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
4
Theoretical and Natural Science, Год журнала: 2025, Номер 82(1), С. 6 - 11
Опубликована: Янв. 15, 2025
In recent years, the agricultural sector has faced immense challenges due to prolonged droughts, excessive soil salinity, and extreme temperature fluctuations, which have collectively stunted crop growth significantly reduced yields. Such environmental stressors not only impact productivity but also pose a substantial threat global food security. To address these challenges, advancements in gene editing introduced CRISPR (clusters of regularly interspaced short palindromic repeats) tools, with CRISPR-Cas9 emerging as particularly powerful widely adopted technology. This tool offers high precision, efficiency, cost-effectiveness, enabling targeted genetic modifications that can enhance resilience facilitate development superior varieties. paper discusses fundamental principles unique characteristics technology, providing comprehensive summary its applications strengthening resistance various abiotic stresses, including drought, temperatures. Additionally, it addresses technology's limitations, outlining areas require further refinement optimize broaden applicability.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 299, С. 140157 - 140157
Опубликована: Янв. 21, 2025
Язык: Английский
Процитировано
0
Frontiers in Plant Science, Год журнала: 2022, Номер 13
Опубликована: Дек. 15, 2022
Among the New Plant Breeding Techniques (NPBTs), CRISPR/Cas9 system represents a useful tool for target gene editing, improving traits of plants rapidly. This technology allows targeting one or more sequences simultaneously, as well introducing new genetic variations by homology-directed recombination. However, remains challenge some polyploid woody species, since all different alleles which mutation is required must be simultaneously targeted. In this work we describe improved protocols adapting to highbush blueberry (Vaccinium corymbosum L.), using Agrobacterium-mediated transformation. As proof concept, targeted encoding phytoene desaturase, whose disrupts chlorophyll biosynthesis allowing visual assessment knockout efficiency. Leaf explants in vitro-cultured cv. Berkeley has been transformed with construct containing two guide RNAs (gRNA1 and gRNA2) conserved regions pds subsequently maintained on selection medium enriched kanamycin. After 4 weeks culture medium, kanamycin-resistant lines were isolated, genotyping these through Sanger sequencing revealed successful editing. Some mutant shoot included albino phenotypes, even if editing efficiencies quite low both gRNAs, ranging between 2.1 9.6% gRNA1 3.0 23.8 gRNA2. Here showed very effective adventitious regeneration protocol commercial cultivar "Berkeley", further improvement use Vaccinium L., opening way breeding mediated biotechnological approaches.
Язык: Английский
Процитировано
16
Horticultural Plant Journal, Год журнала: 2022, Номер 9(5), С. 986 - 998
Опубликована: Дек. 27, 2022
Язык: Английский
Процитировано
16