Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae

Cell Reports, Год журнала: 2022, Номер 38(10), С. 110494 - 110494

Опубликована: Март 1, 2022

Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that ectopic expression an AgOR (AgOR2) ORNs dampened expressing neuron. This contrasts with studies Drosophila melanogaster which non-native ORs confers neuronal responses without interfering native physiology. RNA-seq analyses comparing wild-type antennae to those ectopically AgOR2 indicated nearly all transcripts were significantly downregulated (except for AgOR2). Additional suggest protein rather than mRNA mediates this downregulation. Using situ hybridization, we find gene choice is active into adulthood inhibits AgORs from turning at late stage. Our study shows (in contrast Drosophila) are sensitive a currently unexplored mechanism regulation.

Язык: Английский

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Knockout of the odorant receptor co-receptor, orco, impairs feeding, mating and egg-laying behavior in the fall armyworm Spodoptera frugiperda

Insect Biochemistry and Molecular Biology, Год журнала: 2022, Номер 152, С. 103889 - 103889

Опубликована: Дек. 7, 2022

Язык: Английский

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Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach

iScience, Год журнала: 2022, Номер 25(5), С. 104272 - 104272

Опубликована: Апрель 20, 2022

Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is heteromeric complex composed ligand-specific receptor and the co-receptor (ORco). In this study, we identified ORco gene cockroach, Periplaneta americana (PameORco), performed RNAi-based functional analysis PameORco. All OSNs basiconic sensilla PameORco received including sex pheromones. trichoid sensilla, PameORco-positive OSN was consistently paired with PameORco-negative tuned to acids. adult cockroaches injected dsRNA at nymphal stage, expression PameORco, odor receptions ORs, its central processing were strongly suppressed. These results provide new insights into molecular basis reception cockroach. long-lasting irreversible effects RNAi would be an effective method for controlling household pest.

Язык: Английский

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Neurogenetic identification of mosquito sensory neurons

iScience, Год журнала: 2023, Номер 26(5), С. 106690 - 106690

Опубликована: Апрель 19, 2023

Anopheles mosquitoes, as vectors for the malaria parasite, are a global threat to human health. To find and bite human, they utilize neurons within their sensory appendages. However, identity quantification of appendage lacking. Here we use neurogenetic approach label all in coluzzii mosquitoes. We homology assisted CRISPR knock-in (HACK) generate T2A-QF2w synaptic gene bruchpilot. membrane-targeted GFP reporter visualize brain quantify major chemosensory appendages (antenna, maxillary palp, labella, tarsi, ovipositor). By comparing labeling brp>GFP Orco>GFP predict extent expressing ionotropic receptors (IRs) or other receptors. This work introduces valuable genetic tool functional analysis mosquito neurobiology initiates characterization that guide behavior.

Язык: Английский

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Orcomutagenesis causes deficiencies in olfactory sensitivity and fertility in the migratory brown planthopper,Nilaparvata lugens

Pest Management Science, Год журнала: 2022, Номер 79(3), С. 1030 - 1039

Опубликована: Ноя. 10, 2022

The migratory brown planthopper (BPH), Nilaparvata lugens (Hemiptera: Delphacidae), is the most destructive pest affecting rice plants in Asia and feeds exclusively on rice. Studies have investigated olfactory response of BPHs to major volatile compounds insect co-receptor (Orco) a crucial component system essential for odorant detection. Functional analysis Orco gene would aid identification their host preference.We identified BPH homologue (NlOrco) by Blast searching transcriptome with Drosophila sequence. Spatiotemporal indicated that NlOrco first expressed later egg stage, mainly antennae adult females. A NlOrco-knockout line (NlOrco-/- ) was generated through clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated mutagenesis. NlOrco-/- mutants showed no consequently host-plant preference. In addition, exhibited extended nymphal duration impaired fecundity compared wild-type BPHs.Our findings exhibit strong responses suggest required maximal fitness BPHs. Our results may facilitate potential target genes or chemical control applications. © 2022 Society Chemical Industry.

Язык: Английский

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Neuroregulation of foraging behavior mediated by the olfactory co-receptor Orco in termites

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 262, С. 129639 - 129639

Опубликована: Фев. 7, 2024

Язык: Английский

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Genetic and behavioral differences between above and below ground Culex pipiens bioforms

Heredity, Год журнала: 2024, Номер 132(5), С. 221 - 231

Опубликована: Фев. 29, 2024

Язык: Английский

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The conserved IR75 subfamily mediates carboxylic acid detection in insects of public health and agricultural importance

Journal of Insect Science, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 1, 2025

Insects perceive and respond to carboxylic acids (CAs), amines, aldehydes primarily via conserved ionotropic receptors (IRs). These form the basis for a second olfactory system distinct from well-characterized odorant receptors. Neurons expressing IRs are housed in dedicated sensilla innervate glomeruli, separate those innervated by receptor neurons. The IR8a co-receptor is highly across insect orders and, together with tuning receptors, detects CAs. conservation of genes anatomical separation neural pathways underscore importance these compound classes CAs, specifically chemical ecology. We provide summary acid detection insects, focusing on dipteran lepidopteran species significance public health agriculture. An overview behavior toward CAs provided, as well comprehensive update function insects. Phylogenetic analysis publicly available genome databases reveals several that encode express homologs previously deorphanized highlighting avenues future research.

Язык: Английский

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Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy

Опубликована: Март 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Язык: Английский

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Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy

Опубликована: Март 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Язык: Английский

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