CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Spodoptera frugiperda): Progress and Prospects DOI Creative Commons

Yussuf Mohamed Salum,

Anyuan Yin,

Uroosa Zaheer

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1074 - 1074

Published: Aug. 27, 2024

The fall armyworm (Spodoptera frugiperda) poses a substantial threat to many important crops worldwide, emphasizing the need develop and implement advanced technologies for effective pest control. CRISPR/Cas9, derived from bacterial adaptive immune system, is prominent tool used genome editing in living organisms. Due its high specificity adaptability, CRISPR/Cas9 system has been various functional gene studies through knockout applied research engineer phenotypes that may cause economical losses. practical application of diverse insect orders also provided opportunities developing strategies genetic control, such as drive precision-guided sterile technique (pgSIT). In this review, comprehensive overview recent progress S. frugiperda presented. We outline fundamental principles applying embryonic microinjection highlight study genes associated with biological aspects, including body color, insecticide resistance, olfactory behavior, sex determination, development, RNAi. ability technology induce sterility, disrupt developmental stages, influence mating behaviors illustrates roles management strategies. Furthermore, review addresses limitations studying function explores future potential promising controlling pest.

Language: Английский

Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae DOI Creative Commons

Sarah E. Maguire,

Ali Afify, Loyal A. Goff

et al.

Cell Reports, Journal Year: 2022, Volume and Issue: 38(10), P. 110494 - 110494

Published: March 1, 2022

Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that ectopic expression an AgOR (AgOR2) ORNs dampened expressing neuron. This contrasts with studies Drosophila melanogaster which non-native ORs confers neuronal responses without interfering native physiology. RNA-seq analyses comparing wild-type antennae to those ectopically AgOR2 indicated nearly all transcripts were significantly downregulated (except for AgOR2). Additional suggest protein rather than mRNA mediates this downregulation. Using situ hybridization, we find gene choice is active into adulthood inhibits AgORs from turning at late stage. Our study shows (in contrast Drosophila) are sensitive a currently unexplored mechanism regulation.

Language: Английский

Citations

25
Knockout of the odorant receptor co-receptor, orco, impairs feeding, mating and egg-laying behavior in the fall armyworm Spodoptera frugiperda DOI
Hao Sun,

Ling-Ao Bu,

Shao-Cong Su

et al.

Insect Biochemistry and Molecular Biology, Journal Year: 2022, Volume and Issue: 152, P. 103889 - 103889

Published: Dec. 7, 2022

Language: Английский

Citations

25
Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach DOI Creative Commons
Kosuke Tateishi, Takayuki Watanabe, Hiroshi Nishino

et al.

iScience, Journal Year: 2022, Volume and Issue: 25(5), P. 104272 - 104272

Published: April 20, 2022

Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is heteromeric complex composed ligand-specific receptor and the co-receptor (ORco). In this study, we identified ORco gene cockroach, Periplaneta americana (PameORco), performed RNAi-based functional analysis PameORco. All OSNs basiconic sensilla PameORco received including sex pheromones. trichoid sensilla, PameORco-positive OSN was consistently paired with PameORco-negative tuned to acids. adult cockroaches injected dsRNA at nymphal stage, expression PameORco, odor receptions ORs, its central processing were strongly suppressed. These results provide new insights into molecular basis reception cockroach. long-lasting irreversible effects RNAi would be an effective method for controlling household pest.

Language: Английский

Citations

22
Neurogenetic identification of mosquito sensory neurons DOI Creative Commons
Joanna K. Konopka, Darya Task, Danny Poinapen

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106690 - 106690

Published: April 19, 2023

Anopheles mosquitoes, as vectors for the malaria parasite, are a global threat to human health. To find and bite human, they utilize neurons within their sensory appendages. However, identity quantification of appendage lacking. Here we use neurogenetic approach label all in coluzzii mosquitoes. We homology assisted CRISPR knock-in (HACK) generate T2A-QF2w synaptic gene bruchpilot. membrane-targeted GFP reporter visualize brain quantify major chemosensory appendages (antenna, maxillary palp, labella, tarsi, ovipositor). By comparing labeling brp>GFP Orco>GFP predict extent expressing ionotropic receptors (IRs) or other receptors. This work introduces valuable genetic tool functional analysis mosquito neurobiology initiates characterization that guide behavior.

Language: Английский

Citations

13
The conserved IR75 subfamily mediates carboxylic acid detection in insects of public health and agricultural importance DOI Creative Commons

Matthew Cooke,

Michael Solomon Chembars,

R. Jason Pitts

et al.

Journal of Insect Science, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 1, 2025

Insects perceive and respond to carboxylic acids (CAs), amines, aldehydes primarily via conserved ionotropic receptors (IRs). These form the basis for a second olfactory system distinct from well-characterized odorant receptors. Neurons expressing IRs are housed in dedicated sensilla innervate glomeruli, separate those innervated by receptor neurons. The IR8a co-receptor is highly across insect orders and, together with tuning receptors, detects CAs. conservation of genes anatomical separation neural pathways underscore importance these compound classes CAs, specifically chemical ecology. We provide summary acid detection insects, focusing on dipteran lepidopteran species significance public health agriculture. An overview behavior toward CAs provided, as well comprehensive update function insects. Phylogenetic analysis publicly available genome databases reveals several that encode express homologs previously deorphanized highlighting avenues future research.

Language: Английский

Citations

0
Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy DOI Open Access
Qi Wang, Yufei Jia, Hans M. Smid

et al.

Published: March 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Language: Английский

Citations

0
Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy DOI Open Access
Qi Wang, Yufei Jia, Hans M. Smid

et al.

Published: March 21, 2025

Insect herbivores such as caterpillars, are under strong selection pressure from natural enemies, especially parasitoid wasps. Although the role of olfaction in host-plant seeking has been investigated great detail parasitoids and adult lepidopteran, caterpillar olfactory system its significance tri-trophic interactions remains poorly understood. In this study, we Pieris brassicae caterpillars importance information among herbivore, Brassica oleracea primary enemy Cotesia glomerata . To examine olfaction, utilized CRISPR/Cas9 to knock out odorant receptor co-receptor ( Orco ). This knockout (KO) impaired detection processing brain. KO exhibited reduced weight lost preference for their optimal food plant. Interestingly, also experienced when challenged by C. whose ovipositor had removed, mortality attack unmanipulated increased. We then behavior P. response volatiles plants attacked conspecific on which were themselves After analyzing volatile compounds involved these interactions, concluded that enables locate suitable sources more efficiently well selecting enemy-free spaces. Our results reveal crucial feeding avoidance, highlighting chemoreceptor genes shaping ecological interactions.

Language: Английский

Citations

0
Orcomutagenesis causes deficiencies in olfactory sensitivity and fertility in the migratory brown planthopper,Nilaparvata lugens DOI

Xin‐Yang Liu,

Ru Yan,

Sun‐Jie Chen

et al.

Pest Management Science, Journal Year: 2022, Volume and Issue: 79(3), P. 1030 - 1039

Published: Nov. 10, 2022

The migratory brown planthopper (BPH), Nilaparvata lugens (Hemiptera: Delphacidae), is the most destructive pest affecting rice plants in Asia and feeds exclusively on rice. Studies have investigated olfactory response of BPHs to major volatile compounds insect co-receptor (Orco) a crucial component system essential for odorant detection. Functional analysis Orco gene would aid identification their host preference.We identified BPH homologue (NlOrco) by Blast searching transcriptome with Drosophila sequence. Spatiotemporal indicated that NlOrco first expressed later egg stage, mainly antennae adult females. A NlOrco-knockout line (NlOrco-/- ) was generated through clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated mutagenesis. NlOrco-/- mutants showed no consequently host-plant preference. In addition, exhibited extended nymphal duration impaired fecundity compared wild-type BPHs.Our findings exhibit strong responses suggest required maximal fitness BPHs. Our results may facilitate potential target genes or chemical control applications. © 2022 Society Chemical Industry.

Language: Английский

Citations

16
Neuroregulation of foraging behavior mediated by the olfactory co-receptor Orco in termites DOI
Huan Xu,

Yongyong Gao,

Ali Hassan

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 129639 - 129639

Published: Feb. 7, 2024

Language: Английский

Citations

3
Genetic and behavioral differences between above and below ground Culex pipiens bioforms DOI
Katherine L. Bell,

Anna Noreuil,

Erin K. Molloy

et al.

Heredity, Journal Year: 2024, Volume and Issue: 132(5), P. 221 - 231

Published: Feb. 29, 2024

Language: Английский

Citations

3