Double Peptide-Functionalized Carboxymethyl Chitosan-Coated Liposomes Loaded with Dexamethasone as a Potential Strategy for Active Targeting Drug Delivery

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 922 - 922

Опубликована: Янв. 22, 2025

Liposomes are intensively used as nanocarriers for biology, biochemistry, medicine, and in the cosmetics industry their non-toxic biocompatible nature makes these vesicles attractive systems biomedical applications. Moreover, conjugation of specific ligands to liposomes increases cellular uptake therapeutic efficiency. Considering aspects, aim present study was obtain new formulations cationic coated with dual-peptide functionalized carboxymethyl chitosan (CMCS) treatment inner ear diseases. In order achieve efficient active targeting ensuring a high efficacy treatment, CMCS Tet1 peptide, target cells, TAT ensure penetration. Furthermore, dexamethasone phosphate loaded model drug inflammation. The infrared spectroscopy confirmed functionalization two peptides. mean diameter uncovered varied between 167 198 nm whereas CMCS-coated ranged from 179 202 nm. TEM analysis showed spherical shape unilamellar structure liposomes. release efficiency after 24 h uncoated 37 40% it appeared that modulated this release. obtained results demonstrated hemocompatible since, tested concentration 100 µg/mL, liposome suspension had lysis erythrocytes lower than 2.5% 180 min incubation. addition, peptide-functionalized induced non-significant effect on viability normal V79-4 cells 48 h, at highest doses. Values 71.31% were recorded (CLCP-1), 77.28% (CLCP-2) 74.36% (CLCP-3), correlated cytotoxic effects 28.69%, 22.72%, 25.64%.

Язык: Английский

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Peptide-Functionalized Nanomedicine: Advancements in Drug Delivery, Diagnostics, and Biomedical Applications

Molecules, Год журнала: 2025, Номер 30(7), С. 1572 - 1572

Опубликована: Март 31, 2025

Peptide-functionalized nanomedicine has emerged as a transformative approach in precision therapeutics and diagnostics, leveraging the specificity of peptides to enhance performance nanocarriers, including gold nanoparticles, polymeric liposomes, mesoporous silica quantum dots. These systems enable targeted drug delivery, molecular imaging, biosensing, regenerative medicine, offering unparalleled advantages bioavailability, cellular uptake, therapeutic selectivity. This review provides comprehensive analysis peptide-functionalization strategies, nanocarrier design, their applications across oncology, neurodegenerative disorders, inflammatory diseases, infectious tissue engineering. We further discuss critical role physicochemical characterization, vitro vivo validation, regulatory considerations translating these technologies into clinical practice. Despite rapid progress peptide-functionalized platforms, challenges related stability, immune response, off-target effects, large-scale reproducibility remain key obstacles widespread adoption. Addressing through advanced peptide engineering, optimized synthesis methodologies, harmonization will be essential for integration. By bridging fundamental research with translational advancements, this an interdisciplinary roadmap next generation nanomedicines poised revolutionize therapy diagnostics.

Язык: Английский

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The Evolution of Microfluidic‐Based Drug‐Loading Techniques for Cells and Their Derivatives

Small, Год журнала: 2024, Номер 20(47)

Опубликована: Авг. 17, 2024

Conventional drug delivery techniques face challenges related to targeting and adverse reactions. Recent years have witnessed significant advancements in nanoparticle-based carriers. Nevertheless, concerns persist regarding their safety insufficient metabolism. Employing cells derivatives, such as cell membranes extracellular vesicles (EVs), carriers effectively addresses the associated with nanoparticle However, an essential hurdle remains efficiently loading drugs into these With advancement of microfluidic technology its advantages precise manipulation at micro- nanoscales, well minimal sample loss, it has found extensive application using thereby fostering development drug-loading techniques. This paper outlines characteristics benefits utilizing derivatives provides overview current techniques, particularly those rooted technology. The potential for targeted disease therapy through systems employing is foreseen.

Язык: Английский

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Colloidal Structure Dictates Antimicrobial Efficacy in LL‐37 Self‐Assemblies With Glycerol Monooleate

Small, Год журнала: 2024, Номер unknown

Опубликована: Окт. 15, 2024

Abstract The antimicrobial peptide LL‐37 is a promising alternative to conventional antibiotics combat bacteria in suspension and biofilms. Its self‐assembly with polar lipids suggested improve its potential for therapeutic applications higher stability against degradation bioavailability. This study investigates the of glyceryl monooleate (GMO), establishing link between colloidal structure activity. Small‐angle X‐ray scattering, dynamic light scattering cryogenic transmission electron microscopy show structural transformation from dispersions inverse bicontinuous (cubosomes) multilamellar vesicles direct rod‐like mixed‐micelles upon increasing content GMO. In vitro assays planktonic biofilm cells demonstrate that 128 µg mL −1 GMO cubosomes have no impact on Pseudomonas aeruginosa . Still, reduce Staphylococcus aureus population by ≈ 1‐log after 24 h. Cylindrical micelles formed at LL‐37/GMO 9/1 8/2 decrease 6‐log. activity gradually abolished when encapsulated or cubosomes. They also low antibiofilm efficacy promote biomass These results highlight importance outcomes, providing insights advanced lipid nanocarrier designs.

Язык: Английский

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Dual Loading of Trans-Cinnamaldehyde and Either Paclitaxel or Curcumin in Chitosan Nanoparticles: Physicochemical Characterization and Biological Evaluation Against MDCK and HeLa Cells

Polymers, Год журнала: 2024, Номер 16(21), С. 3087 - 3087

Опубликована: Окт. 31, 2024

Biopolymer chitosan sub-micron particles (CSMPs) were prepared by the ionic gelation technique crosslinked with sodium tripolyphosphate co-loaded trans-cinnamaldehyde (TCIN), and either curcumin (CUR) or paclitaxel (PTX). The size of spherical CSMPs increased from 118 nm to 136 170 after loading TCIN CUR, whereas PTX led a slight decrease (114 nm). Polydispersity indexes all samples smaller than 0.4, indicating monodisperse particles. Zeta potential values higher +40 mV determined, which is direct proof high stability these nanoparticles. release studies in vitro, at pH 6.5 7.4, showed dependence on rate value 6.5. However, CUR was not released probably due strong interactions CS biopolymer chains. Cytotoxicity that systems loaded more cytotoxic for HeLa cancer cells MDCK cells. Moreover, synergistic effect against observed TCIN-PTX-loaded CSMP samples. Sensitivity Index indicated have prospective attraction carry conventional new chemotherapeutic drugs. This study demonstrates vitro efficiency obtained drug delivery system, but vivo are necessary confirm its clinical applications.

Язык: Английский

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Sustainable chitosan-based biomaterials for the future: a review

Polymer Bulletin, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

Язык: Английский

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