Biomedicines,
Год журнала:
2025,
Номер
13(1), С. 122 - 122
Опубликована: Янв. 7, 2025
The
role
of
α-synuclein
(α-syn)
pathology
in
Parkinson's
disease
(PD)
is
well
established;
however,
effective
therapies
remain
elusive.
Two
mechanisms
central
to
PD
neurodegeneration
are
the
intracellular
aggregation
misfolded
α-syn
and
uptake
aggregates
into
neurons.
Cationic
arginine-rich
peptides
(CARPs)
an
emerging
class
molecule
with
multiple
neuroprotective
action,
including
protein
stabilisation.
This
study
characterised
both
cortical
neurons
vitro.
Thereafter,
this
examined
therapeutic
potential
CARP,
R18D
(18-mer
D-arginine),
prevent
aforementioned
pathogenic
processes
through
a
cell-free
thioflavin-T
(ThT)
assay
To
induce
aggregation,
rat
primary
were
exposed
seed
(0.14
μM)
for
2
h
allow
protein,
followed
by
treatment
(0.0625,
0.125,
0.25,
0.5
μM),
subsequent
measurement
48
later
using
homogenous
time-resolved
fluorescence
(HTRF)
assay.
assess
neuronal
uptake,
seeds
covalently
labelled
Alexa-Fluor
488
fluorescent
tag,
pre-incubated
(0.125,
then
24
assessed
via
confocal
microscopy.
It
was
demonstrated
that
significantly
reduced
37.8%
77.7%,
respectively.
Also,
monomers
These
findings
highlight
inhibit
key
pathological
progression.
Journal of Integrative Neuroscience,
Год журнала:
2023,
Номер
22(6)
Опубликована: Окт. 30, 2023
Parkinson’s
disease
(PD)
is
a
common
neurodegenerative
disorder
characterized
by
misfolding
of
α-synuclein.
Clinical
manifestations
include
slowly
developing
resting
tremor,
muscle
rigidity,
bradykinesia
and
abnormal
gait.
The
pathological
mechanisms
underlying
PD
are
complex
yet
to
be
fully
elucidated.
studies
suggest
that
the
onset
gastrointestinal
symptoms
may
precede
motor
in
patients.
microbiota-gut-brain
axis
plays
bidirectional
communication
role
between
enteric
nervous
system
central
system.
This
brain
gut
influenced
neural,
immune
endocrine
systems
related
microbiome.
A
growing
body
evidence
indicates
strong
link
dysregulation
microbiota
PD.
In
this
review,
we
present
recent
progress
understanding
relationship
We
focus
on
microbiota,
unique
changes
observed
microbiome
patients,
impact
these
progression
Finally,
evaluate
current
treatment
strategies
for
PD,
including
probiotics,
fecal
microbial
transplants,
dietary
modifications,
drug
therapies.
Chemical Research in Toxicology,
Год журнала:
2024,
Номер
37(7), С. 1071 - 1085
Опубликована: Июль 3, 2024
Parkinson's
disease
(PD)
affects
more
people
worldwide
than
just
aging
alone
can
explain.
This
is
likely
due
to
environmental
influences,
genetic
makeup,
and
changes
in
daily
habits.
The
develops
a
complex
way,
with
movement
problems
caused
by
Lewy
bodies
the
loss
of
dopamine-producing
neurons.
Some
research
suggests
might
start
gut,
hinting
at
connection
between
these
structures
gut
health
PD
patients.
These
patients
often
have
different
bacteria
metabolites.
Pesticides
are
known
increase
risk
PD,
evidence
showing
they
harm
dopamine
Long-term
exposure
pesticides
food
affect
barrier,
bacteria,
blood-brain
but
exact
link
still
unknown.
review
looks
how
separately
influence
development
progression,
highlighting
harmful
effects
bacteria.
We
examined
interaction
patients,
summarizing
cause
imbalances
resulting
changes,
their
overall
on
prognosis.
Biomedicines,
Год журнала:
2025,
Номер
13(1), С. 122 - 122
Опубликована: Янв. 7, 2025
The
role
of
α-synuclein
(α-syn)
pathology
in
Parkinson's
disease
(PD)
is
well
established;
however,
effective
therapies
remain
elusive.
Two
mechanisms
central
to
PD
neurodegeneration
are
the
intracellular
aggregation
misfolded
α-syn
and
uptake
aggregates
into
neurons.
Cationic
arginine-rich
peptides
(CARPs)
an
emerging
class
molecule
with
multiple
neuroprotective
action,
including
protein
stabilisation.
This
study
characterised
both
cortical
neurons
vitro.
Thereafter,
this
examined
therapeutic
potential
CARP,
R18D
(18-mer
D-arginine),
prevent
aforementioned
pathogenic
processes
through
a
cell-free
thioflavin-T
(ThT)
assay
To
induce
aggregation,
rat
primary
were
exposed
seed
(0.14
μM)
for
2
h
allow
protein,
followed
by
treatment
(0.0625,
0.125,
0.25,
0.5
μM),
subsequent
measurement
48
later
using
homogenous
time-resolved
fluorescence
(HTRF)
assay.
assess
neuronal
uptake,
seeds
covalently
labelled
Alexa-Fluor
488
fluorescent
tag,
pre-incubated
(0.125,
then
24
assessed
via
confocal
microscopy.
It
was
demonstrated
that
significantly
reduced
37.8%
77.7%,
respectively.
Also,
monomers
These
findings
highlight
inhibit
key
pathological
progression.
