Nature Methods, Год журнала: 2024, Номер 21(10), С. 1767 - 1769
Опубликована: Окт. 1, 2024
Язык: Английский
Chemical Reviews, Год журнала: 2024, Номер 124(17), С. 9949 - 10051
Опубликована: Авг. 6, 2024
Molecular oxygen, O
Язык: Английский
Процитировано
12
Frontiers in Neuroscience, Год журнала: 2023, Номер 17
Опубликована: Фев. 20, 2023
The development of three-photon microscopy (3PM) has greatly expanded the capability imaging deep within biological tissues, enabling neuroscientists to visualize structure and activity neuronal populations with greater depth than two-photon imaging. In this review, we outline history physical principles 3PM technology. We cover current techniques for improving performance 3PM. Furthermore, summarize applications various brain regions species. Finally, discuss future neuroscience.
Язык: Английский
Процитировано
17
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Дек. 4, 2023
Due to the size and opacity of vertebrate brains, it has until now been impossible simultaneously record neuronal activity at cellular resolution across entire adult brain. As a result, scientists are forced choose between cellular-resolution microscopy over limited fields-of-view or whole-brain imaging coarse-grained resolution. Bridging gap these spatial scales understanding remains major challenge in neuroscience. Here, we introduce blazed oblique plane perform brain-wide recording an vertebrate. Contrary common belief, find that inferences population near-independent scale: set randomly sampled neurons comparable predictive power as same number macrovoxels. Our work thus links with scope, challenges prevailing view macroscale methods generally inferior microscale techniques underscores value multiscale approaches studying activity.
Язык: Английский
Процитировано
17
Nature, Год журнала: 2024, Номер 631(8019), С. 118 - 124
Опубликована: Июнь 19, 2024
Abstract Locating sound sources such as prey or predators is critical for survival in many vertebrates. Terrestrial vertebrates locate by measuring the time delay and intensity difference of pressure at each ear 1–5 . Underwater, however, physics makes interaural cues very small, suggesting that directional hearing fish should be nearly impossible 6 Yet, has been confirmed behaviourally, although mechanisms have remained unknown decades. Several hypotheses proposed to explain this remarkable ability, including possibility evolved an extreme sensitivity minute differences might compare with particle motion signals 7,8 However, experimental challenges long hindered a definitive explanation. Here we empirically test these models transparent teleost Danionella cerebrum , one smallest 9,10 By selectively controlling motion, dissect sensory algorithm underlying acoustic startles. We find both are indispensable behaviour their relative phase controls its direction. Using micro-computed tomography optical vibrometry, further show D. structures implement mechanism. shares more than 15% living vertebrate species, widespread mechanism inferring
Язык: Английский
Процитировано
6
Current Biology, Год журнала: 2023, Номер 33(24), С. 5467 - 5477.e4
Опубликована: Дек. 1, 2023
Danionella cerebrum (DC) is a promising vertebrate animal model for systems neuroscience due to its small adult brain volume and inherent optical transparency, but the scope of their cognitive abilities remains an area active research. In this work, we established behavioral paradigm study visual spatial navigation in DC investigate navigational capabilities strategies. We initially observed that exhibit strong negative phototaxis groups less so as individuals. Using dark preference motivator, designed task inspired by Morris water maze. Through series environmental cue manipulations, found utilize cues anticipate reward location evidence landmark-based strategies wherein could use both proximal distal cues. When subsets were occluded, capable using distant contextual information solve task, providing allocentric navigation. Without cues, tended seek out direct line sight with at least one while maintaining positional bias toward location. total, our results suggest can be used neural mechanisms underlying cellular resolution imaging across brain.
Язык: Английский
Процитировано
12
The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(4)
Опубликована: Апрель 1, 2025
ABSTRACT Animals integrate information from different sensory modalities as they mature and perform increasingly complex behaviors. This may parallel differential investment in specific brain regions depending on the changing demands of inputs. To investigate developmental changes volume canonical regions, we used third harmonic generation imaging for morphometric analysis forebrain midbrain larval through juvenile adult stages Danionella dracula , a transparent, miniature teleost fish whose is optically accessible throughout its lifespan. Relative to whole‐brain volume, increased or telencephalon, higher order integration center, shows most dramatic increases between 30–60 days postfertilization (dpf) again at 90 dpf animals reach adulthood. The torus longitudinalis (TL), visuomotor also significantly 60 dpf. In contrast, optic tectum (TeO), retinal‐recipient target, progressively decreases 30 dpf, whereas relatively consistent across all semicircularis (TS), secondary auditory mechanosensory lateral line olfactory bulb (OB), direct target epithelium. sum, higher‐order centers (telencephalon, TL) occurs juveniles adulthood (60–90 dpf) exhibit more cognitive tasks, modality‐dominant earlier (TeO) development (TS, OB). Complete optical access ’s lifespan provides unique opportunity how structure over correlates with connectivity, microcircuitry, behavior.
Язык: Английский
Процитировано
0
The Journal of Comparative Neurology, Год журнала: 2024, Номер 532(4)
Опубликована: Апрель 1, 2024
Abstract Comprehensive understanding of interconnected networks within the brain requires access to high resolution information large field views and over time. Currently, methods that enable mapping structural changes entire in vivo are extremely limited. Third harmonic generation (THG) can resolve myelinated structures, blood vessels, cell bodies throughout without need for any exogenous labeling. Together with deep penetration long wavelengths, this enables brain‐mapping fractions small animals Here, we demonstrate THG microscopy allows non‐invasive label‐free an adult vertebrate, Danionella dracula , which is a miniature species cyprinid fish. We show capability multiple regions particular identification major commissural fiber bundles midbrain hindbrain. These features provide readily discernable landmarks navigation regional‐specific neuronal groups even single neurons during experiments. further how technique easily be coupled fluorescence used as comparative tool studies other similar body such zebrafish ( Danio rerio) tetras Trochilocharax ornatus ). This new evidence, building on previous studies, demonstrates size relative transparency, combined unique capabilities microscopy, vertebrate brain.
Язык: Английский
Процитировано
3
Journal of Biological Chemistry, Год журнала: 2024, Номер 300(7), С. 107433 - 107433
Опубликована: Май 31, 2024
Язык: Английский
Процитировано
3
eLight, Год журнала: 2024, Номер 4(1)
Опубликована: Ноя. 1, 2024
Abstract In vivo imaging of large-scale neuronal activity plays a pivotal role in unraveling the function brain's circuitry. Multiphoton microscopy, powerful tool for deep-tissue imaging, has received sustained interest advancing its speed, field view and depth. However, to avoid thermal damage scattering biological tissue, decreases exponentially as depth increases. We present suite innovations optimize three-photon microscopy large field-of-view at depths unreachable by two-photon microscopy. These techniques enable us image activities transgenic animals expressing protein calcium sensors ~ 3.5-mm diameter with single-cell resolution deepest cortical layer mouse brains. further demonstrate simultaneous subcortical brain, whole-brain adult zebrafish. The demonstrated can be integrated into typical multiphoton microscopes enlarge system-level neural circuit research.
Язык: Английский
Процитировано
3
Disease Models & Mechanisms, Год журнала: 2022, Номер 15(12)
Опубликована: Ноя. 18, 2022
Danionella cerebrum is a new vertebrate model that offers an exciting opportunity to visualize dynamic biological processes in intact adult animals. Key advantages of this include its small size, life-long optical transparency, genetic amenability and short generation time. Establishing reliable method for longitudinal vivo imaging D. while maintaining viability will allow in-depth image-based studies various involved development, disease onset progression, wound healing, aging live animal. Here, both prolonged confocal using custom-designed 3D-printed chambers described. Two transgenic lines were created test the system, i.e. Tg(mpeg1:dendra2) Tg(kdrl:mCherry-caax). The first line was used macrophages microglia, second spatial registration. By approach, differences immune cell morphology behavior during homeostasis as well response stab or two-photon-induced brain injury observed fish over course several days.
Язык: Английский
Процитировано
15