tesG expression as a potential clinical biomarker for chronic Pseudomonas aeruginosa pulmonary biofilm infections

BMC Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Март 31, 2025

Pseudomonas aeruginosa infections in the lungs affect millions of children and adults worldwide. To our knowledge, no clinically validated prognostic biomarkers for chronic pulmonary P. exist. Therefore, this study aims to identify potential markers biofilm lung infections. Here, we screened expression 11 regulatory genes (tesG, algD, lasR, lasA, lasB, pelB, phzF, rhlA, rsmY, rsmZ, sagS) associations between clinical status infection. RNA was extracted from 210 sputum samples patients (n = 70) with (mean age; 29.3–56.2 years; 33 female). Strong formation correlated prolonged hospital stays (212.2 days vs. 44.4 days) increased mortality (46.2% (18)). is associated tesG (P 0.001), influencing extended intensive care unit 0.002) or hospitalisation pneumonia risk 0.006), 0.001). Notably, linked modulation systemic inflammatory responses predicts biomass. This provides first dataset levels as a predictive biomarker

Язык: Английский

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Interactions and Implications of Klebsiella pneumoniae with Human Immune Responses and Metabolic Pathways: A Comprehensive Review

Infection and Drug Resistance, Год журнала: 2024, Номер Volume 17, С. 449 - 462

Опубликована: Фев. 1, 2024

Abstract: Klebsiella pneumoniae ( K. ), a significant contributor to the global challenge of antibiotic resistance, is not only ubiquitous component human microbiome but also potent pathogen capable causing spectrum diseases. This review provides thorough analysis intricate interactions between and immune system, elucidating its substantial impact on metabolic processes. We explore mechanisms employed by evade manipulate responses, including molecular mimicry, modulation, biofilm formation. The further investigates bacterium ' s influence pathways, particularly glycolysis, highlighting how these exacerbate disease severity. emergence multidrug-resistant extremely drug-resistant strains within Enterobacteriaceae family has heightened public health crisis, underscoring urgency for comprehensive research. investigate roles host's complement autophagy, cell death mechanisms, various cytokines in combating infections, shedding light areas that warrant academic investigation. Additionally, discusses challenges posed K1- K2-capsule polysaccharides vaccine development due their complex structures adhesive properties. Acknowledging limited availability effective antimicrobials, this advocates exploring alternative approaches such as immunotherapeutics, vaccinations, phage therapy. consolidate current knowledge , covering classical non-classical subtypes, antimicrobial resistance-mediated genes, virulence factors, epidemiological trends isolation resistance rates. advances our understanding underscores imperative ongoing research collaborative efforts develop new prevention treatment strategies against formidable pathogen. Keywords: pathogenic mechanism, drug-resistant, strategy

Язык: Английский

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Novel intranasal phage-CaEDTA-ceftazidime/avibactam triple combination therapy demonstrates remarkable efficacy in treating Pseudomonas aeruginosa lung infection

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 168, С. 115793 - 115793

Опубликована: Окт. 29, 2023

Given the rise of multidrug-resistant (MDR) Pseudomonas aeruginosa infections, alternative treatments are needed. Anti-pseudomonal phage therapy shows promise, but its clinical application is limited due to development resistance and a lack biofilm penetration. Recently, adjuvants like CaEDTA have shown ability enhance effectiveness combined antimicrobial agents. Here, we tested phage-adjuvant combination demonstrated intranasally inhaled (KKP10) + in addition ceftazidime/avibactam (CZA) for chronic P. lung infections. The results emphasize that intranasal inhalation along with can successfully re-sensitize MDR CZA triple treatment. This promising approach potential as respiratory tract

Язык: Английский

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First detection of multidrug-resistant and toxigenic Pasteurella aerogenes in sow vaginal discharge: a novel threat to swine health in Thailand

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 26, 2024

Pasteurella aerogenes has been implicated in reproductive disorders sows, yet its prevalence and characteristics vaginal discharge are not well understood. This study aimed to detect P. sow samples investigate antibiotic resistance profile, toxin genes, toxicity. was isolated from 40% (8/20) of samples. Antimicrobial susceptibility testing revealed universal amoxicillin-clavulanate (4:1), with 87.5% isolates also resistant oxytetracycline, amoxicillin, ceftriaxone, enrofloxacin. The colistin gene mcr-2 detected 75% isolates, while class 1 integron (int1) found 12.5%. pax cluster present isolates. Toxicity assays using Panagrellus redivivus demonstrated dose-dependent effects supernatant containing toxins. represents the first report isolation Thailand. high resistance, presence gene, toxicity toxin-positive suggest that may be an underestimated factor swine health. These findings highlight need for further investigation into role potential impact on production.

Язык: Английский

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Quantitative Analysis of Swertiamarin Content from Fagraea fragrans Leaf Extract using HPLC Technique and its Correlation to Antibacterial Activity

Journal of Current Science and Technology, Год журнала: 2024, Номер 14(2)

Опубликована: Май 2, 2024

The rise in drug resistance poses escalating challenges for antibacterial medications, leading to an urgent demand the exploration and innovation of new drugs. Fagraea fragrans Roxb., belonging Gentianaceae family, is one common herbal medicines which can be found abundantly Southeast Asia. secoiridoid glucoside swertiamarin, major compounds F. leaf, exhibits antimicrobial effects. To guarantee medicinal effectiveness leaves, it essential identify a standardized analytical method quantifying active compound. In this study, optimized HPLC following ICH guideline was validated quantitative analysis swertiamarin content leaf terms linearity (y = 5733.5x - 369.1; R2 0.9999), accuracy (93.57-96.39% recovery), precision (0.91% RSD repeatability precision; 1.19% intermediate precision), limit detection (0.73 µg/mL), quantitation (2.23 specificity (peak purity index 0.999995) robustness (% <1).The maximum wavelength at 238 nm. amount extract conducted from 0.0259 ± 0.0005 g/100 g crude drug. exhibited activity against clinical isolates Enterobacter cloacae, Klebsiella pneumonia, Escherichia coli 0.125 mg/mL, Pseudomonas aeruginosa 0.5, showing minimum inhibitory concentration (MIC) values. Whereas even lower MIC developed effectively determines as chemical marker ensure potential leaves.

Язык: Английский

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Cannabidiol Demonstrates Remarkable Efficacy in Treating Multidrug-Resistant Enterococcus Faecalis Infections In Vitro and In Vivo

Trends in Sciences, Год журнала: 2024, Номер 21

Опубликована: Июль 24, 2024

The growing prevalence of multidrug-resistant Enterococcus faecalis infection has become a global concern. There is demand for alternative antibacterial agents, such as herbal alternatives, Cannabidiol, that are cost-effective, non-toxic and efficient. This study investigates the Minimum Inhibitory Concentration (MIC) planktonic cells Biofilm Eradication (MBEC) biofilm formation in multidrug- resistance isolates E. isolates, particularly focusing cannabidiol activity. with strong presence, vancomycin, levofloxacin, daptomycin displayed high MIC MBEC values. Cannabidiol exhibited significantly lower (1 μg/mL) low (2 μg/mL). Moreover, at concentrations µg/mL), demonstrated notable reductions biovolume, bacterial cell viability colony-forming unit compared to daptomycin. mice treated (100 mg/kg) significant reduction load internal organs increased survival. In conclusion, findings underscore promising antibiofilm properties against faecalis, indicating its potential novel therapeutic agent.

Язык: Английский

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Evaluation of mrkD, pgaC and wcaJ as biomarkers for rapid identification of K. pneumoniae biofilm infections from endotracheal aspirates and bronchoalveolar lavage

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 9, 2024

Abstract Klebsiella pneumoniae has been identified as one of the most important opportunistic pathogens responsible for nosocomial infections. Antibiotic resistance and ability to form biofilms are two main factors involved in persistence Conventional detection methods involve culture isolation identification followed by biofilm assay that takes 48–72 h. Timely biofilm-forming resistant is essential appropriately treat infection with right dose combinations. The present study focuses on evaluating an RT-PCR panel using mrk D, pga C, wca J genes screen K. from ETA/BAL specimens. accurately harboring samples a limit 1 ng/µl total RNA. Representative culture-negative-PCR-positive were subjected metagenomics which reads these confirming specificity RT-PCR. D C act specific whereas acts negative marker . In addition, results correlated well phenotypic assay. This first its kind rapid result this highlights based coupled clinical conditions would be appropriate emerging infections or prevent re-infections settings.

Язык: Английский

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Ceftazidime-Avibactam Combination Therapy versus Monotherapy for the Treatment Carbapenem-Resistant Gram-Negative Bacterial Infections: A Retrospective Observational Study

Infection and Drug Resistance, Год журнала: 2024, Номер Volume 17, С. 1281 - 1289

Опубликована: Март 1, 2024

Since the introduction of ceftazidime-avibactam (CZA) in Chinese market, accumulating clinical evidence has substantiated its efficacy treatment infections caused by carbapenem-resistant gram-negative bacteria (CR-GNB). Nevertheless, an ongoing debate persists concerning choice between monotherapy and combination therapy when devising anti-infection protocols.

Язык: Английский

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Respiratory carriage of hypervirulent Klebsiella pneumoniae by indigenous populations of Malaysia

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Апрель 17, 2024

Abstract Klebsiella pneumoniae is a Gram-negative Enterobacteriaceae that classified by the World Health Organisation (WHO) as Priority One ESKAPE pathogen. South and Southeast Asian countries are regions where both healthcare associated infections (HAI) community acquired (CAI) due to extended-spectrum β-lactamase (ESBL)-producing carbapenem-resistant K. (CRKp) of concern. As can also exist harmless commensal, spread resistance genotypes requires epidemiological vigilance. However there has been no significant study carriage isolates from healthy individuals, particularly in Asia, specially Malaysia. Here we describe genomic analysis respiratory obtained Orang Ulu Asli communities Malaysian Borneo Peninsular Malaysia respectively. The majority were species complex (KpSC) 1 ( n = 53, 89.8%). Four variicola subsp. (KpSC3) two quasipneumoniae similipneumoniae (KpSC4) found. It was discovered 30.2% 16) KpSC1 ST23, 11.3% 6) ST65, 7.5% 4) ST13, 13.2% 7) ST86. Only eight encoded ESBL, but importantly not carbapenemase. Thirteen carried yersiniabactin, colibactin aerobactin, all which harboured rmpADC locus therefore characterised hypervirulent. Co-carriage multiple strains minimal. In conclusion, most KpSC1, one common sequence types previously found cases infection. A proportion hypervirulent (hv Kp ) however antibiotic low.

Язык: Английский

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Clinical Efficacy and Safety Evaluation of Ceftazidime-Avibactam in the Treatment of Klebsiella pneumoniae Infection: A Retrospective Analysis from a Hospital in China

Infection and Drug Resistance, Год журнала: 2023, Номер Volume 16, С. 7227 - 7237

Опубликована: Ноя. 1, 2023

Ceftazidime-avibactam (CAZ-AVI) is a new cephalosporin/β-lactamase inhibitor combination that received clinical approval in China 2019. This study aims to investigate the efficacy and safety of CAZ-AVI treatment Klebsiella pneumoniae (KP) infection hospital, differences among various sites between monotherapy therapy, providing valuable insights for its further application.Patients who used January 2019 April 2023 were identified through hospital information system. Demographic information, details site, KP strain's drug sensitivity report, duration, therapies, adverse reactions (ADR), 28-day survival recorded. Clinical microbiological efficacies analyzed using SPSS 23.0 software compare different therapies.The overall effective response (CR) rate against was 62.13%, with favorable microbial (MR) 65.68% 63.91%. No significant difference occurred CR (P = 0.709 0.862, respectively). The MR abdominal infections slightly lower than other 0.021). CR, MR, therapy present values 0.649, 0.123, 0.280, incidence ADR 1.78%, including increased creatinine, elevated transaminase, hematuria, thrombocytopenia.CAZ-AVI demonstrates good infections. similar across sites, did not show an advantage over monotherapy. Further studies are warranted. It should be noted may induce thrombocytopenia hematuria.

Язык: Английский

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