Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis DOI Creative Commons
Yusuke Mori, Sierra Smith, Jiacheng Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 8, 2024

During inner ear semicircular canal morphogenesis in zebrafish, patterned canal-genesis zones express genes for extracellular matrix component synthesis. These include hyaluronan and the hyaluronan-binding chondroitin sulfate proteoglycan Versican, which are abundant matrices of many developing organs. Charged hyaluronate polymers play a key role through osmotic swelling. However, developmental factor(s) that control synthesis components regulation density swelling unknown. Here, we identify transcription factor, Lmx1b, as positive transcriptional regulator hyaluronan, crucial morphogenesis. We show Versican regulates its protein core, whereas charged side chains contribute to hyaluronate. Versican-tuned properties may be broadly used mechanism with important implications understanding diseases where these impaired, hydrogel engineering tissue regeneration.

Язык: Английский

Restoring articular cartilage: insights from structure, composition and development DOI
Alba Pueyo Moliner, Keita Ito, Frank Zaucke

и другие.

Nature Reviews Rheumatology, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Язык: Английский

Процитировано

2

Regulation of Joint Tissues and Joint Function: Is There Potential for Lessons to Be Learned Regarding Regulatory Control from Joint Hypermobility Syndromes? DOI Open Access
David A. Hart

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1256 - 1256

Опубликована: Янв. 31, 2025

Normal development of joints starts in utero with the establishment a cellular and extracellular matrix template. Following birth, individual joint tissues grow mature response to biochemical mechanical signals, leading coordinated pattern further maturation resulting that functions as an organ system. Each develops matures system defined by biomechanical environment which it will function. For those hypermobility syndromes, either specific genetic mutations or not (i.e., Ehlers–Danlos syndrome, Marfan Loey–Dietz hypermobility-type syndrome), this process is partially compromised, but many aspects tissue function retained such organs form retain partial function, compromised. Comparing characteristics what known regarding development, growth, maturation, stressors puberty, pregnancy, aging without leads conclusion have systems may be compromised via failure undergo possibly defective mechanotransduction. Given breadth involved characterization concept reveal commonalities their impact on inform regulatory normal functional integrity. This review/perspective piece attempt detail comparisons summarize how study aid understanding.

Язык: Английский

Процитировано

1

A comprehensive analysis of two types of xenogeneic bone particles for use in maxillofacial bone regeneration therapies DOI Creative Commons
Olimpia Ortiz‐Arrabal, Mario A. Rodríguez, Jesús Chato‐Astrain

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0323754 - e0323754

Опубликована: Май 19, 2025

Regeneration of maxillofacial bone structures is challenging. One strategy for damage repair involves using filler particles. This study analyzed the regenerative potential deproteinized particles (DP) and collagen-based (CP) to determine effectiveness each biomaterial in repair. Structural analysis scanning electron microscopy 3D showed that DP CP were structurally similar, comprising a heterogeneous mixture varying sizes shapes. Ex vivo analyses, including morphological evaluation, LIVE & DEAD assays, DNA quantification, demonstrated high biocompatibility with human cells both direct indirect contact at 24, 48, 72 hours. Both grafted onto Wistar rats critical mandibular defect two months. Computed tomography revealed significant reduction group, but not compared negative controls without any Histological identified tissue collagen fibers mineralized spots DP, more DP. Histochemistry immunohistochemistry confirmed collagen, proteoglycans, osteocalcin presence regeneration area These results confirm particle types regeneration, particularly CP. Future studies should assess their clinical usefulness patients cleft palate, damage, other applications involving engineering techniques.

Язык: Английский

Процитировано

0

Versican controlled by Lmx1b regulates hyaluronate density and hydration for semicircular canal morphogenesis DOI Creative Commons
Yusuke Mori, Sierra Smith, Jiacheng Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 8, 2024

During inner ear semicircular canal morphogenesis in zebrafish, patterned canal-genesis zones express genes for extracellular matrix component synthesis. These include hyaluronan and the hyaluronan-binding chondroitin sulfate proteoglycan Versican, which are abundant matrices of many developing organs. Charged hyaluronate polymers play a key role through osmotic swelling. However, developmental factor(s) that control synthesis components regulation density swelling unknown. Here, we identify transcription factor, Lmx1b, as positive transcriptional regulator hyaluronan, crucial morphogenesis. We show Versican regulates its protein core, whereas charged side chains contribute to hyaluronate. Versican-tuned properties may be broadly used mechanism with important implications understanding diseases where these impaired, hydrogel engineering tissue regeneration.

Язык: Английский

Процитировано

0