Mesenchymal stem cell-derived exosomes as a new therapeutic strategy for liver diseases DOI Creative Commons
Guohua Lou, Zhi Chen, Min Zheng

и другие.

Experimental & Molecular Medicine, Год журнала: 2017, Номер 49(6), С. e346 - e346

Опубликована: Июнь 1, 2017

The administration of mesenchymal stem cells (MSCs) as a therapy for liver disease holds great promise. MSCs can differentiate into hepatocytes, reduce inflammation, promote hepatic regeneration and secrete protective cytokines. However, the risks iatrogenic tumor formation, cellular rejection infusional toxicity in MSC transplantation remain unresolved. Accumulating evidence now suggests that novel cell-free therapy, MSC-secreted exosomes, might constitute compelling alternative because their advantages over corresponding MSCs. They are smaller less complex than parent and, thus, easier to produce store, they devoid viable cells, present no risk formation. Moreover, immunogenic lower content membrane-bound proteins. This paper reviews biogenesis exosomes physiological functions, highlights specific biochemical potential MSC-derived restoring tissue homeostasis. In addition, we summarize recent advances role various diseases, including fibrosis, acute injury hepatocellular carcinoma. also discusses challenges strategies use exosome-based therapies future. Tiny vesicles secreted by adult could help treat fibrosis cancer. Mesenchymal (MSCs), which be derived from bone marrow, cord blood or other sources, have ability but form tumors rejected immune system, raising safety concerns. review article, Yanning Liu colleagues Zhejiang University Hangzhou, China, discuss how administering small known released offers safer assisting following disease. researchers data mouse models showing therapeutic properties exosomes. further steps needed, such better standardization, allow human clinical trials proceed.

Язык: Английский

The biology , function , and biomedical applications of exosomes DOI Open Access
Raghu Kalluri, Valerie S. LeBleu

Science, Год журнала: 2020, Номер 367(6478)

Опубликована: Фев. 7, 2020

Clinical uses of cellular communication Exosomes are a type extracellular vesicle that contain constituents (protein, DNA, and RNA) the cells secrete them. They taken up by distant cells, where they can affect cell function behavior. Intercellular through exosomes seems to be involved in pathogenesis various disorders, including cancer, neurodegeneration, inflammatory diseases. In Review, Kalluri LeBleu discuss biogenesis disease, highlighting areas more research is needed. also potential clinical applications exosome profiling for diagnostics exosome-mediated delivery therapeutics target disease cells. Science , this issue p. eaau6977

Язык: Английский

Процитировано

7510
Blood-Brain Barrier: From Physiology to Disease and Back DOI Open Access
Melanie D. Sweeney, Zhen Zhao, Axel Montagne

и другие.

Physiological Reviews, Год журнала: 2018, Номер 99(1), С. 21 - 78

Опубликована: Окт. 3, 2018

The blood-brain barrier (BBB) prevents neurotoxic plasma components, blood cells, and pathogens from entering the brain. At same time, BBB regulates transport of molecules into out central nervous system (CNS), which maintains tightly controlled chemical composition neuronal milieu that is required for proper functioning. In this review, we first examine molecular cellular mechanisms underlying establishment BBB. Then, focus on physiology, endothelial pericyte transporters, perivascular paravascular transport. Next, discuss rare human monogenic neurological disorders with primary genetic defect in BBB-associated cells demonstrating link between breakdown neurodegeneration. review effects genes inheritance and/or increased susceptibility Alzheimer's disease (AD), Parkinson's (PD), Huntington's disease, amyotrophic lateral sclerosis (ALS) relation to other pathologies deficits. We next how dysfunction relates deficits majority sporadic AD, PD, ALS cases, multiple sclerosis, neurodegenerative disorders, acute CNS such as stroke, traumatic brain injury, spinal cord epilepsy. Lastly, BBB-based therapeutic opportunities. conclude lessons learned future directions, emphasis technological advances investigate functions living brain, at level, address key unanswered questions.

Язык: Английский

Процитировано

1691
Exosomes: biogenesis, biologic function and clinical potential DOI Creative Commons

Yuan Zhang,

Yunfeng Liu, Haiying Liu

и другие.

Cell & Bioscience, Год журнала: 2019, Номер 9(1)

Опубликована: Фев. 15, 2019

Exosomes are nano-sized biovesicles released into surrounding body fluids upon fusion of multivesicular bodies and the plasma membrane. They were shown to carry cell-specific cargos proteins, lipids, genetic materials, can be selectively taken up by neighboring or distant cells far from their release, reprogramming recipient bioactive compounds. Therefore, regulated formation exosomes, specific makeup cargo, cell-targeting specificity immense biological interest considering extremely high potential exosomes as non-invasive diagnostic biomarkers, well therapeutic nanocarriers. In present review, we outline discuss recent progress in elucidation regulatory mechanisms exosome biogenesis, molecular composition technologies used research. Furthermore, focus on use valuable prognostic biomarkers for cell-lineage state-specific contents, possibilities vehicles drug gene delivery. Exosome research is now its infancy, in-depth understanding subcellular components involved will bring light physiological activities.

Язык: Английский

Процитировано

1673
Extracellular vesicles as a next-generation drug delivery platform DOI Open Access
Inge K. Herrmann, Matthew J. A. Wood, Gregor Fuhrmann

и другие.

Nature Nanotechnology, Год журнала: 2021, Номер 16(7), С. 748 - 759

Опубликована: Июль 1, 2021

Язык: Английский

Процитировано

1372
Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells DOI
Myoung Soo Kim, Matthew J. Haney,

Yuling Zhao

и другие.

Nanomedicine Nanotechnology Biology and Medicine, Год журнала: 2015, Номер 12(3), С. 655 - 664

Опубликована: Ноя. 14, 2015

Язык: Английский

Процитировано

1259
The exosome journey: from biogenesis to uptake and intracellular signalling DOI Creative Commons
Sonam Gurung, Dany Perocheau,

Loukia Touramanidou

и другие.

Cell Communication and Signaling, Год журнала: 2021, Номер 19(1)

Опубликована: Апрель 23, 2021

Abstract The use of exosomes in clinical settings is progressively becoming a reality, as trials testing for diagnostic and therapeutic applications are generating remarkable interest from the scientific community investors. Exosomes small extracellular vesicles secreted by all cell types playing intercellular communication roles health disease transferring cellular cargoes such functional proteins, metabolites nucleic acids to recipient cells. An in-depth understanding exosome biology therefore essential ensure development based investigational products. Here we summarise most up-to-date knowkedge about complex biological journey biogenesis secretion, transport uptake their intracellular signalling. We delineate major pathways molecular players that influence each step physiology, highlighting routes interest, which will be benefit manipulation engineering. highlight main controversies field research: adequate definition, characterisation at plasma membrane. also common identified pitfalls affecting research development. Unravelling physiology key ultimate progression towards applications.

Язык: Английский

Процитировано

1217
Exosomes as therapeutic drug carriers and delivery vehicles across biological membranes: current perspectives and future challenges DOI Creative Commons

Dinh Ha,

Ningning Yang,

Venkatareddy Nadithe

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2016, Номер 6(4), С. 287 - 296

Опубликована: Март 9, 2016

Exosomes are small intracellular membrane-based vesicles with different compositions that involved in several biological and pathological processes. The exploitation of exosomes as drug delivery vehicles offers important advantages compared to other nanoparticulate systems such liposomes polymeric nanoparticles; non-immunogenic nature due similar composition body׳s own cells. In this article, the origin structure well their functions outlined. We will then focus on specific applications pharmaceutical development. An overview challenges faced when using a also be discussed.

Язык: Английский

Процитировано

1170
Extracellular vesicles for drug delivery DOI
Pieter Vader, Emma A. Mol, Gerard Pasterkamp

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2016, Номер 106, С. 148 - 156

Опубликована: Фев. 28, 2016

Язык: Английский

Процитировано

1068
Engineering exosomes as refined biological nanoplatforms for drug delivery DOI Creative Commons
Xin Luan, Kanokwan Sansanaphongpricha,

Ila Myers

и другие.

Acta Pharmacologica Sinica, Год журнала: 2017, Номер 38(6), С. 754 - 763

Опубликована: Апрель 10, 2017

Exosomes, a subgroup of extracellular vesicles (EVs), have been recognized as important mediators long distance intercellular communication and are involved in diverse range biological processes. Because their ideal native structure characteristics, exosomes promising nanocarriers for clinical use. Exosomes engineered at the cellular level under natural conditions, but successful exosome modification requires further exploration. The focus this paper is to summarize passive active loading approaches, well specific modifications examples delivery therapeutic imaging molecules. Examples derived from variety origins also provided. biocompatible characteristics exosomes, with suitable modifications, can increase stability efficacy probes therapeutics while enhancing uptake. Challenges translation exosome-based platforms different cell sources advantages each reviewed discussed.

Язык: Английский

Процитировано

974
<p>Exosome: A Review of Its Classification, Isolation Techniques, Storage, Diagnostic and Targeted Therapy Applications</p> DOI Creative Commons
Yi Zhang,

Jia-Yao Bi,

Jiayi Huang

и другие.

International Journal of Nanomedicine, Год журнала: 2020, Номер Volume 15, С. 6917 - 6934

Опубликована: Сен. 1, 2020

Exosomes are nano-sized small extracellular vesicles secreted by cells, carrying nucleic acids, proteins, lipids and other bioactive substances to play a role in the body's physiological pathological processes. Compared synthetic carriers such as liposomes nanoparticles, endogeneity heterogeneity of exosomes give them extensive unique advantages field disease diagnosis treatment. However, storage stability, low yield, purity, weak targeting limit its clinical application. For this reason, further exploration is needed optimize above problems facilitate future functional studies exosomes. In paper, origin, classification, preparation characterization, stability applications exosome delivery system summarized discussed searching large number literatures.

Язык: Английский

Процитировано

956