Abstract
The
primary
function
of
the
kidneys
is
to
maintain
systemic
homeostasis
(disruption
renal
structure
and
results
in
multilevel
impairment
body
function).
Kidney
diseases
are
characterized
by
a
chronic,
progressive
course
may
result
development
chronic
kidney
disease
(CKD).
Evaluation
composition
proteome
urinary
small
extracellular
vesicles
(sEVs)
as
so‐called
liquid
biopsy
promising
new
research
direction.
Knowing
sEV
could
allow
localization
cellular
changes
specific
sections
nephron
or
interstitial
tissue
before
fixed
changes,
detectable
only
at
an
advanced
stage
disease,
occur.
Research
currently
underway
on
role
sEVs
diagnosis
monitoring
many
entities.
Reports
literature
subject
include:
diabetic
nephropathy,
focal
glomerulosclerosis
glomerulopathies,
fibrosis
various
etiologies.
Studies
pediatric
patients
still
few,
involving
piloting
if
groups
without
validation
studies.
Here,
we
review
addressing
use
for
most
common
disorders
children.
We
evaluate
clinical
utility
define
limitations
markers
present
potential
biopsy.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(19), С. 11312 - 11312
Опубликована: Сен. 25, 2022
Extracellular
vesicles
(EVs)
are
nanosized
lipid
bilayer
that
released
by
almost
all
cell
types.
They
range
in
diameter
from
30
nm
to
several
micrometres
and
have
the
ability
carry
biologically
active
molecules
such
as
proteins,
lipids,
RNA,
DNA.
EVs
natural
vectors
play
an
important
role
many
physiological
pathological
processes.
The
amount
composition
of
human
biological
fluids
serve
biomarkers
used
for
diagnosing
diseases
monitoring
effectiveness
treatment.
promising
use
therapeutic
agents
drug
delivery.
However,
successful
clinical
practice
requires
understanding
their
biodistribution
organism.
Numerous
studies
conducted
so
far
on
show
that,
after
intravenous
administration,
mostly
localized
organs
rich
blood
vessels
associated
with
reticuloendothelial
system,
liver,
lungs,
spleen,
kidneys.
In
order
improve
resolution,
new
dyes
labels
being
developed
detection
methods
optimized.
this
work,
we
review
available
modern
approaches
assess
EVs,
well
discuss
advantages
limitations.
Pharmaceutics,
Год журнала:
2023,
Номер
15(2), С. 365 - 365
Опубликована: Янв. 21, 2023
The
article
is
divided
into
several
sections,
focusing
on
extracellular
vesicles’
(EVs)
nature,
features,
commonly
employed
methodologies
and
strategies
for
their
isolation/preparation,
characterization/visualization.
This
work
aims
to
give
an
overview
of
advances
in
EVs’
extensive
nanomedical-drug
delivery
applications.
Furthermore,
considerations
EVs
translation
clinical
application
are
summarized
here,
before
the
review
a
special
kind
vesicles,
ones
derived
from
red
blood
cells
(RBCEVs).
Generally,
employing
as
drug
carriers
means
managing
entities
with
advantageous
properties
over
synthetic
vehicles
or
nanoparticles.
Besides
fact
that
certain
also
reveal
intrinsic
therapeutic
characteristics,
regenerative
medicine,
nanosize,
lipidomic
proteomic
profiles
enable
them
pass
biologic
barriers
display
cell/tissue
tropisms;
indeed,
engineering
can
further
optimize
organ
targeting.
In
second
part
review,
we
focus
our
attention
RBCEVs.
First,
describe
biogenesis
composition
those
naturally
produced
by
(RBCs)
under
physiological
pathological
conditions.
Afterwards,
discuss
current
procedures
isolate
and/or
produce
RBCEVs
lab
load
specific
cargo
exploitation.
Finally,
disclose
most
recent
applications
at
vitro
preclinical
research
level
potential
industrial
conclusion,
be,
near
future,
very
promising
versatile
platform
pharmaceutical
exploitations.
Antioxidants,
Год журнала:
2025,
Номер
14(2), С. 177 - 177
Опубликована: Фев. 3, 2025
A
variety
of
molecular
and
cellular
changes
distinguish
the
multifaceted
biological
process
aging.
Recent
studies
in
this
decade
have
demonstrated
essential
role
extracellular
vesicles
(EVs)
aging
process.
Mitochondrial
malfunction
increased
oxidative
stress
are
major
contributors
for
This
review
investigates
EVs
intercellular
communication,
tissue
regeneration,
inflammation
context
We
also
discuss
exosome
its
utility
to
reduce
stress,
which
is
a
key
part
aging,
as
well
possibility
using
exosomes
anti-aging
drugs.
Changes
cargo
composition
can
influence
phenotype
impact
functionality
cells
tissues.
Additionally,
during
addresses
potential
treatment
strategies
development
biomarkers
age-associated
disorders.
The
highlighted
providing
antioxidant
properties,
help
excessive
reactive
oxygen
species
(ROS)
strengthen
defenses
against
stress.
it
emphasized
age-related
pathologies,
such
neurodegenerative
diseases,
cardiovascular
disorders,
immunosenescence,
offering
insights
into
targeted
interventions
promoting
healthy
article
provides
comprehensive
analysis
current
body
knowledge
regarding
therapeutic
effects
on
with
particular
emphasis
implications
emerging
field
research
relationship
Materials Today Bio,
Год журнала:
2023,
Номер
23, С. 100810 - 100810
Опубликована: Сен. 22, 2023
Diabetic
wounds
(DWs)
pose
a
major
challenge
for
the
public
health
system
owing
to
their
high
incidence,
complex
pathogenesis,
and
long
recovery
time;
thus,
there
is
an
urgent
need
develop
innovative
therapies
accelerate
healing
process
of
diabetic
wounds.
As
natural
nanovesicles,
extracellular
vesicles
(EVs)
are
rich
in
sources
with
low
immunogenicity
abundant
nutritive
molecules
exert
potent
therapeutic
effects
on
wound
healing.
To
avoid
rapid
removal
EVs,
suitable
delivery
required
controlled
release.
Owing
advantages
porosity,
good
biocompatibility,
adjustable
physical
chemical
properties
hydrogels,
EV
biopotentiated
hydrogels
can
aid
achieving
precise
favorable
therapy
against
This
review
highlights
different
design
strategies,
effects,
mechanisms
hydrogels.
We
also
discussed
future
challenges
opportunities
using
Nanoscale,
Год журнала:
2023,
Номер
15(26), С. 10904 - 10938
Опубликована: Янв. 1, 2023
This
review
summarizes
recent
advances
in
extracellular
vesicle-based
carriers
for
glioblastoma
diagnosis
and
therapy,
points
out
the
current
challenges
perspectives.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 3, 2024
Abstract
Glomerulonephritis
(GN)
is
the
most
common
cause
of
end-stage
renal
failure
worldwide;
in
cases,
it
cannot
be
cured
and
can
only
delay
progression
disease.
At
present,
main
treatment
methods
include
symptomatic
therapy,
immunosuppressive
replacement
therapy.
However,
effective
GN
hindered
by
issues
such
as
steroid
resistance,
serious
side
effects,
low
bioavailability,
lack
precise
targeting.
With
widespread
application
nanoparticles
medical
treatment,
novel
have
emerged
for
kidney
diseases.
Targeted
transportation
drugs,
nucleic
acids,
other
substances
to
tissues
even
cells
through
nanodrug
delivery
systems
reduce
systemic
effects
adverse
reactions
drugs
improve
effectiveness.
The
high
specificity
enables
them
bind
ion
channels
block
or
enhance
channel
gating,
thus
improving
inflammation.
This
review
briefly
introduces
characteristics
GN,
describes
status
systematically
summarizes
research
achievements
primary
diabetic
nephropathy
lupus
nephritis,
analyzes
recent
therapeutic
developments,
outlines
promising
directions,
gas
signaling
molecule
ultrasmall
nanoparticles.
current
summarized
provide
a
reference
better
future.
Graphical
npj Regenerative Medicine,
Год журнала:
2024,
Номер
9(1)
Опубликована: Янв. 13, 2024
Abstract
Renal
interstitial
fibrosis
(RIF)
is
a
fundamental
pathological
feature
of
chronic
kidney
disease
(CKD).
However,
toxicity
and
poor
renal
enrichment
inhibitors
limit
their
further
applications.
In
this
study,
platform
for
CKD
therapy
developed
using
superparamagnetic
iron
oxide
nanoparticles
(SPION)
decorated
mesenchymal
stem
cells
derived
extracellular
vesicles
with
carboxyl
terminus
Hsc70-interacting
protein
(CHIP)
high
expression
(SPION-EVs)
to
achieve
higher
renal-targeting
antifibrotic
therapeutic
effect.
SPION-EVs
selectively
accumulate
at
the
injury
sites
under
an
external
magnetic
field.
Moreover,
deliver
CHIP
induce
Smad2/3
degradation
in
tubular
which
alleviates
activation-mediated
fibrosis-like
changes
collagen
deposition.
The
vesicle
engineering
technology
provides
potential
nanoplatform
RIF
through
CHIP-mediated
degradation.
Kidney Research and Clinical Practice,
Год журнала:
2023,
Номер
42(2), С. 174 - 187
Опубликована: Март 31, 2023
The
increasing
prevalence
of
chronic
kidney
disease
(CKD)
is
a
major
global
public
health
concern.
Despite
the
complicated
pathogenesis
CKD,
renal
fibrosis
represents
most
common
pathological
condition,
comprised
progressive
accumulation
extracellular
matrix
in
diseased
kidney.
Over
last
several
decades,
tremendous
progress
understanding
mechanism
has
been
achieved,
and
corresponding
potential
therapeutic
strategies
targeting
fibrosis-related
signaling
pathways
are
emerging.
Importantly,
vesicles
(EVs)
contribute
significantly
to
inflammation
by
mediating
cellular
communication.
Increasing
evidence
suggests
EV-based
therapy
fibrosis,
which
may
represent
future
direction
for
CKD
therapy.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Май 17, 2024
Recent
studies
have
demonstrated
a
strong
association
between
acute
kidney
injury
(AKI)
and
chronic
disease
(CKD),
while
the
unresolved
inflammation
is
believed
to
be
driving
force
for
this
transition
process.
As
transmembrane
pattern
recognition
receptor,
Mincle
(macrophage-inducible
C-type
lectin,
Clec4e)
was
identified
participate
in
early
immune
response
after
AKI.
However,
impact
of
on
AKI
remains
largely
unclear.
We
performed
single-cell
RNA
sequencing
(scRNA-seq)
with
unilateral
ischemia-reperfusion
(UIR)
murine
model
at
days
1,
3,
14
28
injury.
Potential
effects
mechanism
renal
fibrosis
were
further
validated
vivo
utilizing
knockout
mice.
The
dynamic
expression
macrophages
neutrophils
throughout
from
CKD
observed.
For
both
cell
types,
significantly
up-regulated
day
1
following
AKI,
second
rise
observed
14.
Notably,
we
distinct
subclusters
Minclehigh
that
exhibited
time-dependent
influx
dual
peaks
characterized
remarkable
pro-inflammatory
pro-fibrotic
functions.
Moreover,
represented
an
"aged"
mature
neutrophil
subset
derived
"fresh"
cluster
kidney.
Additionally,
synergistic
whereby
Mincle-expressing
sustained
by
tumor
necrosis
factor
(TNF)
production.
Mincle-deficient
mice
reduced
present
findings
unveiled
combined
persistence
during
AKI-to-CKD
transition,
contributing
followed
via
TNF-α
as
central
cytokine.
Targeting
may
offer
novel
therapeutic
strategy
preventing
CKD.
