Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Авг. 27, 2024
The
concept
of
Yin-Yang,
originating
in
ancient
Chinese
philosophy,
symbolizes
two
opposing
but
complementary
forces
or
principles
found
all
aspects
life.
This
can
be
quite
fitting
the
context
extracellular
vehicles
(EVs)
and
inflammatory
diseases.
Over
past
decades,
numerous
studies
have
revealed
that
EVs
exhibit
dual
sides,
acting
as
both
pro-
anti-inflammatory
agents,
akin
to
Yin-Yang
theory
(i.e.,
sides
a
coin).
has
enabled
serve
potential
indicators
pathogenesis
manipulated
for
therapeutic
purposes
by
influencing
immune
pathways.
review
delves
into
recent
advances
understanding
their
regulation
specific
We
shed
light
on
current
prospects
engineering
treating
conditions.
principle
bestows
upon
them
great
as,
therapeutic,
preventive
agents
International Journal of Nanomedicine,
Год журнала:
2023,
Номер
Volume 18, С. 2873 - 2890
Опубликована: Май 1, 2023
Abstract:
With
the
improvement
of
average
life
expectancy
and
increasing
incidence
obesity,
burden
liver
disease
is
increasing.
Liver
a
serious
threat
to
human
health.
Currently,
transplantation
only
effective
treatment
for
end-stage
disease.
However,
still
faces
unavoidable
difficulties.
Mesenchymal
stem
cells
(MSCs)
can
be
used
as
an
alternative
therapy
disease,
especially
cirrhosis,
failure,
complications.
MSCs
may
have
potential
tumorigenic
effects.
Exosomes
derived
from
(MSC-Exos),
important
intercellular
communication
mode
MSCs,
contain
various
proteins,
nucleic
acids,
DNA.
MSC-Exos
delivery
system
treat
diseases
through
immune
regulation,
apoptosis
inhibition,
regeneration
promotion,
drug
delivery,
other
ways.
Good
histocompatibility
material
exchangeability
make
new
diseases.
This
review
summarizes
latest
research
on
vehicles
in
different
diseases,
including
injury,
fibrosis,
hepatocellular
carcinoma
(HCC),
ischemia
reperfusion
injury.
In
addition,
we
discuss
advantages,
disadvantages,
clinical
application
prospects
MSC-Exos-based
vectors
Keywords:
exosomes,
mesenchymal
cells,
nanocarriers
Med,
Год журнала:
2024,
Номер
5(2), С. 148 - 168.e8
Опубликована: Фев. 1, 2024
BackgroundAdipose
tissue-derived
stem
cell-derived
apoptotic
bodies
(ADSC-ABs)
have
shown
great
potential
for
immunomodulation
and
regeneration,
particularly
in
diabetic
wound
therapy.
However,
their
local
application
has
been
limited
by
unclear
regulatory
mechanisms,
rapid
clearance,
short
tissue
retention
times.MethodsWe
analyzed
the
key
role
molecules
pathways
of
ADSC-ABs
regulating
inflammatory
macrophages
mRNA
sequencing
microRNA
(miRNA)
then
verified
them
gene
knockdown.
To
prevent
we
employed
microfluidics
technology
to
prepare
methacrylate-anhydride
gelatin
(GelMA)
microspheres
(GMS)
controlled
release
ABs.
Finally,
evaluated
effectiveness
ADSC-AB-laden
GMSs
(ABs@GMSs)
a
rat
model.FindingsOur
results
demonstrated
that
effectively
balanced
macrophage
polarization
through
janus
kinase
(JAK)-signal
transducer
activator
transcription
(STAT)
pathway,
mediated
miR-20a-5p.
Furthermore,
showed
AB@GMSs
had
good
biocompatibility,
significantly
delayed
clearance
ABs,
ameliorated
inflammation
promoted
vascularization,
thus
facilitating
its
healing.ConclusionsOur
study
reveals
mechanism
balancing
highlightsthe
importance
delaying
GMSs.
These
findings
important
implications
development
novel
therapies
healing.FundingThis
research
was
supported
National
Key
Research
Development
Program
China
(2020YFA0908200),
Natural
Science
Foundation
(82272263,
82002053,
32000937,
82202467),
Shanghai
"Rising
Stars
Medical
Talents"
Youth
(22MC1940300),
Municipal
Health
Commission
(20204Y0354),
Technology
Funds
(22YF1421400).
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 2241 - 2264
Опубликована: Март 1, 2024
Abstract:
Recently,
gene
therapy
has
become
a
subject
of
considerable
research
and
been
widely
evaluated
in
various
disease
models.
Though
it
is
considered
as
stand-alone
agent
for
COVID-19
vaccination,
still
suffering
from
the
following
drawbacks
during
its
translation
bench
to
bedside:
high
sensitivity
exogenous
nucleic
acids
enzymatic
degradation;
severe
side
effects
induced
either
by
or
components
formulation;
difficulty
cross
barriers
before
reaching
therapeutic
target.
Therefore,
successful
application
therapy,
safe
reliable
transport
vector
urgently
needed.
Extracellular
vesicles
(EVs)
are
ideal
candidate
delivery
drugs
owing
their
low
immunogenicity,
good
biocompatibility
toxicity.
To
better
understand
properties
EVs
advantages
drug
vehicles,
this
review
covers
origin
methods
generation,
well
common
isolation
purification
research,
with
pros
cons
discussed.
Meanwhile,
engineering
also
highlighted.
In
addition,
paper
presents
progress
EVs-mediated
microRNAs,
small
interfering
RNAs,
messenger
plasmids,
antisense
oligonucleotides.
We
believe
will
provide
theoretical
basis
development
drugs.
Keywords:
extracellular
vesicles,
system,
Macromolecular Rapid Communications,
Год журнала:
2024,
Номер
45(11)
Опубликована: Фев. 24, 2024
Abstract
Hydrogels,
key
in
biomedical
research
for
their
hydrophilicity
and
versatility,
have
evolved
with
hydrogel
microspheres
(HMs)
of
micron‐scale
dimensions,
enhancing
role
minimally
invasive
therapeutic
delivery,
tissue
repair,
regeneration.
The
recent
emergence
nanomaterials
has
ushered
a
revolutionary
transformation
the
field,
which
demonstrates
tremendous
potential
targeted
therapies,
biological
imaging,
disease
diagnostics.
Consequently,
integration
advanced
nanotechnology
promises
to
trigger
new
revolution
realm
hydrogels.
HMs
loaded
combine
advantages
both
hydrogels
nanomaterials,
enables
multifaceted
functionalities
such
as
efficient
drug
sustained
release,
therapy,
lubrication,
biochemical
detection,
medical
biosensing
monitoring,
micro‐robotics.
Here,
this
review
comprehensively
expounds
upon
commonly
used
classifications.
Then,
it
provides
comprehensive
insights
into
raw
materials
preparation
methods
HMs.
Besides,
common
strategies
employed
achieve
nano‐micron
combinations
are
summarized,
latest
applications
these
combined
field
elucidated.
Finally,
valuable
future
design
development
provided.
Biological Procedures Online,
Год журнала:
2023,
Номер
25(1)
Опубликована: Ноя. 9, 2023
Abstract
Extracellular
vesicles
(EVs)
are
nanoscale
derived
from
cells
that
mediate
intercellular
communication
by
transporting
bioactive
molecules.
They
play
significant
roles
in
various
physiological
and
pathological
conditions.
EVs
hold
great
potential
as
novel
biomarkers
of
diseases,
therapeutic
agents,
drug
delivery
vehicles.
Furthermore,
vehicles
have
demonstrated
advantages
preclinical
settings.
In
this
review,
we
discussed
the
biogenesis
characteristics
their
functions
cancer.
We
summarize
applications
a
natural
cancer
therapy.
highlight
existing
challenges,
illuminate
vital
questions,
propose
recommendations
to
effectively
address
them
effectively.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(8), С. 7287 - 7287
Опубликована: Апрель 14, 2023
Extracellular
vesicles
(EVs)
are
membrane
released
into
the
extracellular
milieu
by
cells
of
various
origins.
They
contain
different
biological
cargoes,
protecting
them
from
degradation
environmental
factors.
There
is
an
opinion
that
EVs
have
a
number
advantages
over
synthetic
carriers,
creating
new
opportunities
for
drug
delivery.
In
this
review,
we
discuss
ability
to
function
as
carriers
therapeutic
nucleic
acids
(tNAs),
challenges
associated
with
use
such
in
vivo,
and
strategies
tNA
loading
EVs.
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июль 22, 2024
Abstract
Stroke
is
a
leading
cause
of
mortality
and
long-term
disability
globally,
with
acute
ischemic
stroke
(AIS)
being
the
most
common
subtype.
Despite
significant
advances
in
reperfusion
therapies,
their
limited
time
window
associated
risks
underscore
necessity
for
novel
treatment
strategies.
Stem
cell-derived
extracellular
vesicles
(EVs)
have
emerged
as
promising
therapeutic
approach
due
to
ability
modulate
post-stroke
microenvironment
facilitate
neuroprotection
neurorestoration.
This
review
synthesizes
current
research
on
potential
stem
EVs
AIS,
focusing
origin,
biogenesis,
mechanisms
action,
strategies
enhancing
targeting
capacity
efficacy.
Additionally,
we
explore
innovative
combination
therapies
discuss
both
challenges
prospects
EV-based
treatments.
Our
findings
reveal
that
exhibit
diverse
effects
such
promoting
neuronal
survival,
diminishing
neuroinflammation,
protecting
blood-brain
barrier,
angiogenesis
neurogenesis.
Various
strategies,
including
modifications
cargo
modifications,
been
developed
improve
efficacy
EVs.
Combining
other
treatments,
therapy,
cell
transplantation,
nanomedicine,
gut
microbiome
modulation,
holds
great
promise
improving
outcomes.
However,
heterogeneity
need
standardized
protocols
EV
production
quality
control
remain
be
addressed.
represent
avenue
offering
address
limitations
Further
needed
optimize
translate
benefits
clinical
practice,
an
emphasis
ensuring
safety,
overcoming
regulatory
hurdles,
specificity
delivery
target
tissues.
Graphical
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 7, 2024
Inflammation
is
a
key
pathological
feature
of
many
diseases,
disrupting
normal
tissue
structure
and
resulting
in
irreversible
damage.
Despite
the
need
for
effective
inflammation
control,
current
treatments,
including
stem
cell
therapies,
remain
insufficient.
Recently,
extracellular
vesicles
secreted
by
adipose-derived
cells
(ADSC-EVs)
have
garnered
attention
their
significant
anti-inflammatory
properties.
As
carriers
bioactive
substances,
these
demonstrated
potent
capabilities
modulating
promoting
repair
conditions
such
as
rheumatoid
arthritis,
osteoarthritis,
diabetes,
cardiovascular
stroke,
wound
healing.
Consequently,
ADSC-EVs
are
emerging
promising
alternatives
to
conventional
ADSC-based
offering
advantages
reduced
risk
immune
rejection,
enhanced
stability,
ease
storage
handling.
However,
specific
mechanisms
which
regulate
under
not
fully
understood.
This
review
discusses
role
impact
on
disease
prognosis,
potential
promote
repair.
Additionally,
it
provides
insights
into
future
clinical
research
focused
ADSC-EV
therapies
inflammatory
overcome
some
limitations
associated
with
cell-based
therapies.
