Extracellular Vesicle, Год журнала: 2024, Номер 4, С. 100059 - 100059
Опубликована: Дек. 1, 2024
Язык: Английский
Biomarker Research, Год журнала: 2025, Номер 13(1)
Опубликована: Янв. 26, 2025
Abstract Macrophages are pivotal in the body’s defense and response to inflammation. They present significant numbers widely implicated various diseases, including cancer. While molecular histological techniques have advanced our understanding of macrophage biology, their precise function within cancerous microenvironments remains underexplored. Enhancing knowledge macrophages dynamics extracellular vesicles (EVs) cancer development can potentially improve therapeutic management. Notably, also been harnessed deliver drugs. Noninvasive vivo imaging is crucial for investigating intricate cellular processes, comprehending underlying mechanisms tracking cells EVs’ migration, devising macrophage-dependent drug-delivery systems living organisms. Thus, has become an indispensable tool biomedical research. The integration multimodal approaches continued novel contrast agents hold promise overcoming current limitations expanding applications imaging. This study comprehensively reviews several methods labeling modalities, assessing merits drawbacks each approach. review concludes by offering insights into applicability real time monitoring preclinical clinical scenarios.
Язык: Английский
Процитировано
2
ACS Omega, Год журнала: 2024, Номер 9(6), С. 6184 - 6218
Опубликована: Янв. 31, 2024
Extracellular vesicles have emerged as promising tools in regenerative medicine due to their inherent ability facilitate intercellular communication and modulate cellular functions. These nanosized transport bioactive molecules, such proteins, lipids, nucleic acids, which can affect the behavior of recipient cells promote tissue regeneration. However, therapeutic application these is frequently constrained by rapid clearance from body inability maintain a sustained presence at injury site. In order overcome obstacles, hydrogels been used extracellular vesicle delivery vehicles, providing localized controlled release system that improves efficacy. This Review will examine role vesicle-loaded regeneration, discussing potential applications, current challenges, future directions. We investigate origins, composition, characterization techniques vesicles, focusing on recent advances exosome profiling machine learning this field. addition, we properties make them ideal carriers. Recent studies utilizing combination for regeneration be highlighted, comprehensive overview research landscape
Язык: Английский
Процитировано
12
Biomaterials, Год журнала: 2024, Номер 311, С. 122694 - 122694
Опубликована: Июнь 28, 2024
Язык: Английский
Процитировано
8
The International Journal of Lower Extremity Wounds, Год журнала: 2024, Номер unknown
Опубликована: Апрель 15, 2024
Nanohydrogel wound healing refers to the use of nanotechnology-based hydrogel materials promote wounds. Hydrogel dressings are made up a three-dimensional network hydrophilic polymers that can absorb and retain large amounts water or other fluids. Nanohydrogels take this concept further by incorporating nanoscale particles structures into matrix. These nanoparticles be various materials, such as silver, zinc oxide, derived from natural substances like chitosan. The inclusion provide additional properties benefits dressings. designed release bioactive substances, growth factors drugs, in controlled manner. This allows for targeted delivery therapeutics site, promoting reducing inflammation. Nanoparticles reinforce structure hydrogels, improving their mechanical strength stability. often incorporate antimicrobial nanoparticles, silver oxide. have shown effective activity against wide range bacteria, fungi, pathogens. By them dressings, nanohydrogels help prevent reduce risk infection encapsulate factors, peptides, sustained therapeutic agents promotes facilitating cell proliferation, inflammation, supporting tissue regeneration. unique nanohydrogels, including ability maintain moist environment deliver agents, accelerate process. creating an optimal repair, faster more efficient
Язык: Английский
Процитировано
7
Biomaterials Science, Год журнала: 2024, Номер 12(5), С. 1131 - 1150
Опубликована: Янв. 1, 2024
Extracellular vesicles (EVs) are with lipid bilayer structures shed from the plasma membrane of cells. Microvesicles (MVs) a subset EVs containing proteins, lipids, nucleic acids, and other metabolites. MVs can be produced under specific cell stimulation conditions isolated by modern separation technology. Due to their tumor homing large volume, cell-derived microvesicles (TMVs) have attracted interest recently become excellent delivery carriers for therapeutic vaccines, imaging agents or antitumor drugs. However, preparing sufficient high-purity TMVs conducting clinical transformation has challenge in this field. In review, recent research achievements generation, isolation, characterization, modification, application cancer therapy reviewed, challenges facing applications also highlighted.
Язык: Английский
Процитировано
4
Proteomes, Год журнала: 2025, Номер 13(1), С. 12 - 12
Опубликована: Март 4, 2025
Extracellular vesicles (EVs) are lipid-bound secreted by cells, including exosomes, microvesicles, and apoptotic bodies. Proteomic analyses of EVs, particularly in relation to cancer, reveal specific biomarkers crucial for diagnosis therapy. However, isolation techniques such as ultracentrifugation, size-exclusion chromatography, ultrafiltration face challenges regarding purity, contamination, yield. Contamination from other proteins complicates downstream processing, leading difficulties identifying interpreting results. Future research will focus on refining EV characterization diagnostic therapeutic applications, improving proteomics tools greater accuracy, exploring the use EVs drug delivery regenerative medicine. In this review, we provide a bird’s eye view various challenges, starting with methods, yield, limitations proteome analysis protein targets.
Язык: Английский
Процитировано
0
Journal of Extracellular Vesicles, Год журнала: 2025, Номер 14(3)
Опубликована: Март 1, 2025
ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162902 - 162902
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Pharmaceutics, Год журнала: 2024, Номер 16(3), С. 326 - 326
Опубликована: Фев. 26, 2024
Microneedles (MNs), renowned for their painless and minimally invasive qualities, exhibit significant potential facilitating effective drug delivery, vaccination, targeted sample extraction. Extracellular vesicles (EVs), serving as cargo MNs, are naturally occurring nanovesicles secreted by cells characterized novel biomarkers, low immunogenicity, cell-source-specific traits. MNs prove instrumental in extracting EVs from the fluid, thereby a promising diagnostic prognostic tool. To harness therapeutic of tissue repair, with sustained delivery leverage micron-sized channels to enhance site concentration, demonstrating efficacy treating various diseases, such Achillea tendinopathy, hair loss, spinal cord injury, diabetic ulcers. EV-loaded emerge platform repair applications skin, cardiac, tendon, hair, tissues. This review commences an overview subsequently delving into role MNs. The paper then synthesizes latest advancements use regenerative extending research progress disease diagnosis evaluations. It aims offer valuable insights forecast future trajectories hope inspiring innovative ideas among researchers this field.
Язык: Английский
Процитировано
3
Biologics, Год журнала: 2024, Номер 4(1), С. 88 - 104
Опубликована: Март 6, 2024
Extracellular vesicles (EVs) encompass a diverse array of cell-derived vesicles, originating either from the endosomal compartment (exosomes) or generated through shedding cell membrane. These lipid bilayer nanovesicles carry cargo consisting nucleic acids, various macromolecules, and growth factors, capable being assimilated by nearby distant cells biofluids, thereby triggering wide range cellular responses. Given their distinctive biological characteristics crucial roles in intercellular communication, EVs have garnered significant attention, especially concerning potential clinical applications. Inheriting parent cells, present promising resources for disease biomarkers. Research elucidating specific impacts on target has sparked enthusiasm therapeutic potential. Compelling evidence indicates that RNA housed within can modulate gene expression influence functions recipient cells. However, despite progress, numerous aspects EV biology remain obscure, encompassing selective cargo-loading mechanisms yield distinct compositions source variability size content, undisclosed pathways governing uptake fate A thorough understanding core mechanisms—such as generation, trafficking, payload delivery—is essential effective utilization. This review explores current loading transportation EVs, light advancements made toward
Язык: Английский
Процитировано
2