Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 31, 2024
Abstract
The
translation
of
cell‐derived
extracellular
vesicles
(EVs)
into
biogenic
gene
delivery
systems
is
limited
by
relatively
inefficient
loading
strategies.
In
this
work,
the
various
nucleic
acids
small
EVs
via
their
spontaneous
hybridization
with
preloaded
non‐lamellar
liquid
crystalline
lipid
nanoparticles
(LCNPs),
forming
hybrid
(HEVs)
described.
It
demonstrated
that
LCNPs
undergo
pH‐dependent
structural
transitions
from
inverse
hexagonal
(
H
II
)
phases
at
pH
5
to
more
disordered
phases,
possibly
micellar
L
2
or
sponge
3
7.4,
which
are
particularly
suitable
for
inducing
a
controlled
process
EVs.
State‐of‐the‐art
single‐particle
analysis
techniques
reveal
interact
EV
subpopulations
physiological
conditions
and
≈40%
HEVs
loaded
genetic
cargo.
Importantly,
study
demonstrates
membrane
proteins
remain
accessible
on
HEV
surfaces,
intrinsic
enzymatic
activity
unaffected
after
process.
Finally,
show
in
vitro
improved
transfection
efficiencies
compared
unhybridized
LCNPs.
summary,
versatile
platform
holds
potential
acid
molecules
native
may
help
developing
EV‐based
therapeutics.
Extracellular
vesicles
(EVs)
are
cell
derived
nanovesicles
which
implicated
in
both
physiological
and
pathological
intercellular
communication,
including
the
initiation,
progression,
metastasis
of
cancer.
The
exchange
biomolecules
between
stromal
cells
cancer
via
EVs
can
provide
a
window
to
monitor
development
real
time
for
better
diagnostic
interventional
strategies.
In
addition,
process
secretion
internalization
by
tumor
microenvironment
(TME)
be
exploited
delivering
therapeutics.
have
potential
targeted,
biocompatible,
efficient
delivery
platform
treatment
other
diseases.
Natural
as
well
engineered
nanomedicine
immense
disease
intervention.
Here,
we
an
overview
current
knowledge
EVs'
function
therapeutic
applications
setting,
EV
engineering
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Янв. 30, 2025
Abstract
Extracellular
vesicles
(EVs)
are
taken
up
by
most
cells,
however
specific
or
preferential
cell
targeting
remains
a
hurdle.
This
study
aims
to
develop
an
EV
that
targets
cells
involved
in
inflammation,
specifically
those
expressing
intercellular
adhesion
molecule-1
(ICAM-1).
To
target
these
we
overexpress
the
ICAM-1
binding
receptor
“lymphocyte
function-associated
antigen-1”
(LFA-1)
HEK293F
sequential
transfection
of
plasmids
two
LFA-1
subunits,
ITGAL
and
ITGB2
(CD11a
CD18).
The
was
strongly
overexpressed
on
EVs
released
transfected
cells.
We
further
loaded
with
therapeutic
peptide,
myeloid
differentiation
primary
response
88
(Myd88;
Myd88
),
through
developed
open-and-close
procedure.
is
downstream
common
intracellular
messenger
for
TLR-receptors.
expression
increases
ICAM-1-expressing
effect
dose-dependently
inhibited
neutralizing
antibody.
Further,
activated
human
endothelial
treated
had
increased
uptake
EVs,
resulting
dose-dependent
inhibition
induced
release
IL-8,
presumably
Myd88.
conclude
LFA-1-expressing
may
be
candidate
suitable
delivering
peptides
inflammatory
diseases
associated
TLR-activation.
Graphical
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 1, 2024
Exosomes
play
a
crucial
role
in
various
biological
processes,
such
as
human
development,
immune
responses,
and
disease
occurrence.
The
membrane
proteins
on
exosomes
are
pivotal
factors
for
their
functionality.
Currently,
numerous
have
been
identified
exosome
membranes,
participating
intercellular
communication,
mediating
target
cell
recognition,
regulating
processes.
Furthermore,
from
derived
cancer
cells
can
serve
relevant
biomarkers
early
diagnosis.
This
article
provides
comprehensive
review
of
the
composition
diverse
functions
organism's
Through
in-depth
exploration
proteins,
it
is
expected
to
offer
essential
foundations
future
development
novel
biomedical
diagnostics
therapies.
ACS Nano,
Год журнала:
2024,
Номер
18(34), С. 23117 - 23135
Опубликована: Авг. 17, 2024
The
escalating
prevalence
of
obesity
poses
significant
health
challenges
due
to
its
direct
association
with
various
diseases.
Most
existing
medications,
such
as
appetite
suppressants
and
fat
absorption
inhibitors,
suffer
from
limited
effectiveness
undesirable
side
effects.
Here,
inspired
by
the
versatile
metabolic
effects
turmeric,
we
developed
a
naturally
derived
nanoformulation
"Reconstructed
Turmeric-derived
Nanovesicles
(Rec-tNVs)"
for
treatment.
Employing
quantitative
nanoflow
cytometry,
four-orders-of-magnitude
increase
in
curcumin
content
(∼10
Journal of Agriculture and Food Research,
Год журнала:
2024,
Номер
18, С. 101370 - 101370
Опубликована: Авг. 25, 2024
The
polyphenol
resveratrol
(RSV)
undergoes
extensive
phase
II
metabolism,
which
limits
its
bioavailability
and
bioactivity,
including
anticancer
effects.
Growing
preclinical
evidence
reinforces
milk-derived
exosomes
(EXOs)
as
promising
nanocarrier
drugs
bioactives
delivery
systems.
Herein,
to
overcome
the
mentioned
RSV's
drawbacks,
EXOs
were
loaded
with
RSV
(EXO-RSV)
evaluate
brain
in
Sprague-Dawley
rats
vitro
antiproliferative
effects
against
human
neuroblastoma
SH-SY5Y
glioblastoma
U-87MG
cancer
cells
compared
non-encapsulated
RSV.
Pharmacokinetic
analyses
perfused
brains
showed
detection
only
after
EXO-RSV
administration,
not
administration.
Encapsulation
also
resulted
lower
concentrations
of
II-derived
metabolites
circulation.
Additionally,
blood-brain
barrier
(BBB)
transport
assays
using
microvascular
endothelial
(HBMECs)
corroborated
that
encapsulation
enhanced
efficiency
protected
it
from
cellular
metabolism.
In
silico
phase-II
conjugates,
primarily
3-glucuronide,
capacity
than
cross
BBB.
Finally,
(47−750
nM)
an
increased
dose-dependent
efficacy
on
both
cell
lines
(10
μM),
was
ineffective
72
h
treatment.
Our
findings
suggest
protect
metabolism
enhance
activity.
Biomedicines,
Год журнала:
2025,
Номер
13(1), С. 147 - 147
Опубликована: Янв. 9, 2025
Human
immunodeficiency
virus
(HIV)
establishes
latent
infections
in
cellular
reservoirs,
including
microglia.
HC69
cells,
a
microglial
model
of
HIV
latency,
contain
an
promoter
long
terminal
repeat
(LTR)-GFP
reporter
and
were
used
for
testing
the
efficacy
two-step
magnetoelectric
nanoparticle
(MENP)
extracellular
vesicle
(xEV)
latency-targeting
(MELT)
nanotherapeutic.
GFP
expression
at
rest
is
low
(GFPLo),
upon
exposure
to
LTR,
transcription-activating
agents
(i.e.,
TNF-α)
are
induced
be
high
expressing
(GFPHi).
The
first
step
MELT
utilized
ZL0580,
Tat
inhibitor
loaded
into
EVs
(80%)
via
incubation.
ZL0580-EVs
taken
up
by
GFPLo
blocked
LTR
transcriptional
reactivation
50%
90%
less
toxic
than
ZL0580
alone.
second
involved
conjugation
monomethyl
auristatin
E
(MMAE)
MENPs.
HPLC
measurements
showed
80%
MMAE
attachment
Flow
cytometry-based
membrane
potential
indicated
that
membranes
GFPHi
60%
more
polarized
cells.
More
MMAE-MENPs
internalized
HC69.
Using
mixed-cell
blood-brain
barrier
(BBB)
Transwell
model,
we
demonstrated
20%
crossed
BBB,
was
reduced
10%.
Overall,
this
study
can
potentially
as
nanotherapeutic
target
latency
