Phytochemical Analysis,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 20, 2024
ABSTRACT
Introduction
Drug
discovery
research
targeting
SARS‐CoV‐2
and
other
emerging
pathogens
remains
critically
important.
Active
compounds
derived
from
plants
frequently
serve
as
lead
for
further
drug
discovery;
however,
numerous
unrelated
chemical
constituents
in
crude
extracts
may
obscure
the
effective
ingredients
LC‐MS
analysis.
Objective
The
aim
of
this
study
is
to
construct
a
biolayer
interferometry
(BLI)‐based
system
recognizing
active
that
inhibit
main
protease
(Mpro)
identify
components
binding
Mpro
herbal
medicines.
Methodology
We
developed
novel
FRET
fluorogenic
probe
by
linking
amino
acid
sequences
fluorescent
proteins
Lssmorange
mKate2
(Ls‐mK).
interaction
between
traditional
Chinese
medicine
was
analyzed
using
BLI.
Ultrahigh
performance
liquid
chromatography
coupled
with
quadrupole
time‐of‐flight
mass
spectrometry
(UPLC‐QTOF‐MS)
employed
analyze
composition
Results
Fluorescence
detection
spectroscopy
confirmed
successful
construction
an
inhibitor
screening
system.
Lanqin
Oral
Liquid
(LQL)
Gardeniae
fructus
exhibited
strong
inhibitory
effects
on
Mpro.
Ten
were
identified
G.
extracts;
among
them,
deacetyl
asperulosidic
methyl
ester
(DAAME)
Gardoside
found
strongly
bind
Mpro,
dissociation
constants
(KD)
3.41
μM
801
nM,
respectively.
half‐maximal
concentrations
(IC50)
DAAME
27.46
13.7
μM,
Conclusion
This
established
functional
Among
10
displayed
activity,
indicating
their
potential
inhibiting
viral
replication.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 27, 2025
Anxiety
disorders
and
depression
often
co-occur
lack
broadly
available
treatments.
Gardenia
extract
significantly
associated
with
treatment
of
anxiety-like
depression.
Based
on
the
dose
effect
hypothesis
previous
studies,
it
is
speculated
that
crocin
I,
main
component
gardenia,
related
to
The
present
study
aimed
verify
reversal
I
chronic
corticosterone-induced
depression,
further
explore
its
metabolic
process
in
vivo.
Ultimately,
a
new
method
for
rapid
sensitive
detection
trace
substances
was
established.
In
this
study,
rat
model
induced
by
corticosterone.
effects
were
explored
combining
behavioral,
pathological
sections
ELASA
data.
It
first
time
can
reverse
morphological
changes
hippocampus
corticosterone
rats.
terms
behavior,
Ⅰ
improve
depressive
behavior
exhibited
rats
water
maze
sugar
preference
experiments.
also
repair
neuronal
cell
damage
Dentate
gyrus,
CA1,
CA3
areas
hippocampus.
regulates
expression
levels
monoamine
neurotransmitters
brain,
thereby
exerting
an
anti-anxiety-like
effect.
Pharmacokinetic
analysis
performed
determine
Further
integrating
Surface-Enhanced
Raman
Scattering
(SERS)
technology,
highly
had
been
discovered
hippocampal
morphology
caused
determined
exert
therapeutic
regulating
brain.
vivo
pharmacokinetic
experiments
revealed
could
not
pass
through
intestinal
barrier
into
blood,
but
metabolite
crocetin
blood.
Finally,
synthesizing
silver
nanoparticles,
amounts
blood
samples
established
time.The
calculated
enhancement
factor
4.49
×
1011.
stable
reproducible
over
week.
This
series
studies
great
potential
treating
comorbid
anxiety
shortens
distance
from
theoretical
research
clinical
application.
Cosmetics,
Год журнала:
2025,
Номер
12(2), С. 72 - 72
Опубликована: Апрель 9, 2025
Gardeniae
Fructus
(GF),
the
desiccative
mature
fruitage
of
Gardenia
jasminoides
J.
Ellis
(G.
jasminoides),
is
a
traditional
herbal
medicine
in
China
with
potential
value
against
skin
photodamage.
However,
phytochemical
basis
and
mechanisms
underlying
GF’s
anti-photodamage
effects
remain
unclear.
In
this
study,
chemical
components
GF
extract
(GFE)
were
analyzed
using
ultra-high-performance
liquid
chromatography
coupled
tandem
mass
spectrometry
(UPLC-MS/MS),
iridoids
identified
as
main
components.
The
antioxidant,
anti-inflammatory,
barrier-repair
GFE
UVB-induced
photodamage
assessed
through
vitro
experiments.
Additionally,
predicted
proteomics
network
pharmacology.
results
showed
that
significantly
increased
levels
total
superoxide
dismutase
(T-SOD),
catalase
(CAT),
glutathione
peroxidase
(GSH-Px)
induced
by
UVB,
while
decreasing
reactive
oxygen
species
(ROS)
malondialdehyde
(MDA)
contents.
also
inhibited
secretion
interleukin-6
(IL-6)
interleukin-1β
(IL-1β).
upregulated
expression
filaggrin
(FLG),
loricrin
(LOR),
involucrin
(IVL)
3D
epidermal
models.
Proteomic
analysis
pharmacology
indicated
iridoid
ameliorated
damage
probably
regulating
cell
cycle-related
proteins
signaling
pathways,
though
requires
further
experimental
confirmation.
Overall,
provide
essential
evidence
to
support
development
skincare
active
ingredient.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 27, 2024
Gardeniae
Fructus
(
Zhizi
)
serves
as
both
a
medicinal
and
edible
substance
finds
widespread
use
in
various
industries.
There
are
often
two
kinds
of
materials
the
market:
Shuizhizi
.
Typically,
with
small,
round
fruit
is
used
for
purposes,
while
,
characterized
by
large,
elongated
fruit,
employed
dyeing.
Market
surveys
have
revealed
diverse
range
types,
modern
research
indicates
that
contains
rich
chemical
components
pharmacological
activities.
In
this
study,
we
collected
25
batches
samples,
categorizing
them
based
on
appearance
into
obovate
fruits,
seven
length
grades
(A–G).
Using
ultra-high
performance
liquid
chromatography
coupled
triple
quadrupole
mass
spectrometry
(UHPLC-QQQ-MS/MS)
method,
simultaneously
quantified
13
main
fruits
Gardenia
species.
addition,
compared
weight
percentage
pericarp,
flesh,
seeds
parts
samples
different
traits,
parts.
Results
indicated
that,
aside
from
few
instances
overlapping
size
ranges,
generally
exhibits
larger
longer
dimensions
than
The
proportion
pericarp
higher
pericarp.
Quantitative
results
highlighted
significant
differences
component
content
between
containing
levels
iridoids.
PCA
OPLS-DA
analysis
distinctly
divided
among
which
three
iridoids,
organic
acids,
one
flavonoid
made
contributions
to
their
classification.
Cluster
heatmap
also
demonstrated
complete
separation
clear
distinctions
origins.
distribution
remained
consistent,
iridoids
pigments
concentrated
acids
enriched
summary,
study
contributes
valuable
insights
classifying
offers
guidance
rational
Phytochemical Analysis,
Год журнала:
2024,
Номер
unknown
Опубликована: Май 28, 2024
Abstract
Introduction
Fructus
Gardeniae
(ZZ),
a
traditional
Chinese
herb,
has
been
used
in
treating
patients
with
jaundice,
inflammation,
etc.
When
mixed
ginger
juice
and
stir‐baked,
juice‐processed
(JZZ)
is
produced,
the
chemical
compositions
ZZ
would
be
changed
by
adding
juice.
Objective
To
illuminate
differential
components
between
JZZ.
Methods
HPLC,
UHPLC‐Q‐TOF‐MS,
Heracles
NEO
ultra‐fast
gas
phase
electronic
nose
were
applied
to
identify
Results
HPLC
fingerprints
of
JZZ
established,
24
common
peaks
found.
The
content
determination
results
showed
that
contents
shanzhiside,
geniposidic
acid,
genipin‐1‐β‐D‐gentiobioside
geniposide
increased,
while
crocin
I
II
decreased
By
twenty‐six
possible
inferred,
among
which
11
different.
In
further
investigation,
eight
identified
as
distinctive
non‐volatile
compounds
nose,
four
substances
inferred
volatile
Conclusion
Shanzhiside,
caffeic
genipin‐1‐β‐D‐gentiobioside,
geniposide,
rutin,
I,
II,
4‐Sinapoyl‐5‐caffeoylquinic
acid
Aniline,
3‐methyl‐3‐sulfanylbutanol‐1‐ol,
E‐3‐octen‐2‐one,
decyl
propaonate
This
experiment
explored
simple
approach
objective
stable
results,
provide
new
ideas
for
studying
decoction
pieces
similar
morphological
appearance,
especially
those
different
odors.
Biomedical Chromatography,
Год журнала:
2024,
Номер
38(8)
Опубликована: Май 28, 2024
Abstract
The
present
study
examined
the
pharmacokinetics
of
IMM‐H012
in
rat
plasma,
utilizing
ultra‐performance
liquid
chromatography‐tandem
mass
spectrometry
(UPLC‐MS/MS).
Internal
standard
cilostazol
was
employed,
and
plasma
samples
were
processed
using
acetonitrile
precipitation.
A
mobile
phase
(acetonitrile–0.1%
formic
acid
water)
with
gradient
elution
used
to
achieve
chromatographic
separation
a
UPLC
BEH
C18
column.
In
multiple
reaction
monitoring
mode,
electrospray
ionization
MS/MS
utilized
positive
mode.
Based
on
findings,
lower
limit
quantification
2
ng/mL,
linearity
found
be
acceptable
within
range
2–2000
ng/mL
(
R
>
0.995).
intra‐day
inter‐day
precision
relative
deviation
less
than
14%
plasma.
matrix
effect
102%–107%,
accuracy
ranged
from
92%
113%.
Pharmacokinetics
rats
after
oral
administration
successfully
studied
UPLC‐MS/MS.
