Ferrostatin‑1 alleviates liver injury via decreasing ferroptosis following ricin toxin poisoning in rat

Toxicology, Год журнала: 2024, Номер 503, С. 153767 - 153767

Опубликована: Март 1, 2024

Ricin is a highly toxic plant toxin that can cause multi-organ failure, especially liver dysfunction, and potential bioterrorism agent. Despite the serious public health challenge posed by ricin, effective therapeutic for ricin-induced poisoning currently unavailable. Therefore, it important to explore mechanism of ricin develop appropriate treatment protocols accordingly. Previous studies have shown lipid peroxidation iron accumulation are associated with poisoning. Ferroptosis an iron-dependent form cell death caused excessive peroxide. The role ferroptosis in unclear require further study. We investigated effect on injury elucidated mechanism. results showed occurred ricin-intoxicated rats, Ferrostatin‑1 could ameliorate hepatic thus injury. induced decreasing reduced glutathione protein level peroxidase 4 Solute Carrier Family 7 Member 11, increasing iron, malondialdehyde reactive oxygen species, mitochondrial damage, whereas pretreatment increased decreased malondialdehyde, ameliorated thereby alleviated These suggested exacerbated after inhibition may be novel strategy

Язык: Английский

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Intestinal microbiota promoted NiONPs-induced liver fibrosis via effecting serum metabolism

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 270, С. 115943 - 115943

Опубликована: Янв. 1, 2024

Nickel oxide nanoparticles (NiONPs) are toxic heavy metal compounds that induce liver fibrosis and metabolic disorders. Current research shows the intestinal microbiota regulates metabolism through gut-liver axis. However, it is unclear whether NiONPs affect relationship between Therefore, in this study, we established model by administering 0.015, 0.06 0.24 mg/mL tracheal instillation twice a week for 9 weeks rats, then collected serum fecal sample whole metabolomics metagenomic sequencing. As result of sequencing, screened out seven metabolites (beta-D-glucuronide, methylmalonic acid, linoleic phosphotidylcholine, lysophosphatidylinositol, docosapentaenoic acid progesterone) related to functional alterations (p < 0.05), obtained decrease probiotics abundances 0.05) as well variation enzyme activity indicating inhibited proliferation probiotics. correlation analysis, found positive differential probiotics, such lysophosphatidylinositol was positively correlated with Desulfuribacillus, Jeotgallibacillus Rummeliibacillus 0.05). We also had correlations proteins enzymes microbiota, glucarate dehydratase, dihydroorotate dehydrogenase acetyl-CoA carboxylase Finally, six pathways both were involved, pentose glucuronate interconversions, metabolism. In vitro experiments showed increased transcriptional expression Col1A1 LX-2 cells, while reducing mRNA serine/threonine activators, acetyl coenzyme carboxylase, synthase, short chain fatty sodium butyrate can alleviate these trends. The results proved systems associated metabolites, suggesting disturbance reduction promoted occurrence development NiONPs-induced affecting pathways.

Язык: Английский

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SAP130 mediates crosstalk between hepatocyte ferroptosis and M1 macrophage polarization in PFOS-induced hepatotoxicity

The Science of The Total Environment, Год журнала: 2024, Номер 951, С. 175612 - 175612

Опубликована: Авг. 19, 2024

Язык: Английский

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Thiacloprid exposure disrupts the gut-liver axis and induces liver dysfunction in the Reeves' turtles (Mauremys reevesii)

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 289, С. 117710 - 117710

Опубликована: Янв. 1, 2025

As one of the neonicotinoid insecticides, thiacloprid (THI) is extensively used in agriculture and frequently detected various aquatic environments, posing a potential threat to organisms. However, effects THI exposure on turtles remain unknown. In this study, we focused investigating whether has toxic effect gut-liver axis turtles. The Reeves' (Mauremys reevesii) were exposed 0.0178 μM, 6 60 μM for 5 consecutive weeks. results revealed that altered composition intestinal flora, with decrease relative abundance Romboutsia, an increase Clostridium_sensu_stricto_1, Cetobacterium, Enterococcus. This disruption barrier led lipopolysaccharide (LPS), THI, other harmful substances entering liver. Metabolomic transcriptomic analyses indicated metabolic dysregulation differences gene expression concentrated amino acid metabolism lipid metabolism, ultimately resulting severe liver damage steatosis. Furthermore, elevated levels function indicators, including aspartate aminotransferase (AST), alanine (ALT), alkaline phosphatase (ALP), total bile (TBA), triglyceride (TG), positively correlated increased concentrations. Our findings demonstrate impairs causes dysfunction turtles, providing new insights into evaluating

Язык: Английский

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Cardioprotective effects of Dendrobium officinale polysaccharides on thiacloprid-induced cardiac injury via modulating mitochondrial dynamics

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142497 - 142497

Опубликована: Март 1, 2025

Язык: Английский

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The impact of subchronic ozone exposure on serum metabolome and the mechanisms of abnormal bile acid and arachidonic acid metabolisms in the liver

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 252, С. 114573 - 114573

Опубликована: Янв. 25, 2023

Ambient ozone (O3) pollution can induce respiratory and cardiovascular toxicity. However, its impact on the metabolome underlying mechanisms remain unclear. This study first investigated serum metabolite changes in rats exposed to 0.5 ppm O3 for 3 months using untargeted metabolomic approach. Results showed chronic exposure significantly altered levels of 34 metabolites with potential increased risk digestive, disease. Moreover, bile acid synthesis secretion, arachidonic (AA) metabolism became most prominent affected metabolic pathways after exposure. Further studies found that elevated toxic was not due biosynthesis liver, but reduced reuptake from portal vein hepatocytes owing repressed Ntcp Oatp1a1, decreased efflux as a results inhibited Bsep, Ostalpha Ostbeta. Meanwhile, expressions detoxification enzyme SULT2A1 important regulators FXR, PXR HNF4α also contributed abnormal acids. In addition, promoted conversion AA into thromboxane A2 (TXA2) 20-hydroxyarachidonic (20-HETE) liver by up-regulation Fads2, Cyp4a Tbxas1 which resulting linoleic (LA), B2 (TXB2) 20-HETE serum. Furthermore, apparent hepatic inflammation, fibrosis function were ozone-exposed rats. These indicated could alter interfering their inducing injury aggravate disorders.

Язык: Английский

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Luteolin-β-CD-MOF prevents against acetaminophen-mediated liver damage by controlling ferroptosis through GSH/GPX4/SLC7A11 signal axis

Journal of Functional Foods, Год журнала: 2024, Номер 116, С. 106138 - 106138

Опубликована: Март 18, 2024

Excess acetaminophen can result in hepatotoxicity, while luteolin has the anti-liver injury property. However, low water solubility reduces its pharmacological performance. Luteolin-β-Cyclodextrin-Metal-Organic-Framework (Luteolin-β-CD-MOF) is an alternative with more water-soluble activity of luteolin. In this study, we utilized vivo and vitro methods to explore underlying protective mechanism luteolin-β-CD-MOF acetaminophen-induced liver injury. Untargeted metabolomics were performed, along study bile acids metabolism injured rats. Western blotting assays performed assess expressions ferroptosis-related proteins (glutathione peroxidase 4 (GPX4), ferritin heavy chain-1 (FTH1), heme oxygenase-1 (HO-1), solute carrier family 7 member 11 (SLC7A11)) rats L02 cells. The findings demonstrated that intervention ameliorated restored dysregulated by mitigating ferroptosis through GSH/GPX4/SLC7A11 signal axis. This research offers novel perspectives on acetaminophen-liver prevention strategies.

Язык: Английский

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Triterpenoids from Ilicis Rotundae Cortex ameliorate hyperlipidemia by affecting bile acids-hepatointestinal FXR axis

Phytomedicine, Год журнала: 2025, Номер 139, С. 156537 - 156537

Опубликована: Фев. 20, 2025

Язык: Английский

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Purple Yam Anthocyanin Forestalling Lipopolysaccharide‐Induced Intestine Damage by Selectively Promoting Lactococcus

Food Frontiers, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

ABSTRACT The mechanism of anthocyanin in alleviating intestine injury pigs is worth pursuing due to the higher similarity physiological structure between human beings and than rodents. Herein, we investigated by which improves gut damage induced with lipopolysaccharide further confirmed this using fecal microbiota transplantation. Thirty‐two piglets were randomly divided into four treatments: control group (Control), model (LPS), prophylactic (An‐LPS), transplantation (FMT‐LPS). results showed that oxidative stress increased expression p‐p65, Bax, Keap1 decreased Nrf2, Occludin, Claudin‐1 jejunum. However, its suspension reversed these influences. Meanwhile, selectively improved certain microbiota, including Lactococcus , leading distinct metabolic profiles. These differences favor activating antioxidant‐related signaling pathway, Nrf2/Keap1 inhibiting pro‐inflammatory pathway such as NF‐κB, alleviated inflammation significantly intestinal tight junction, Occludin Claudin‐1. Moreover, compared LPS, serum antioxidant enzyme activity IL‐10 content An‐LPS. In conclusion, ameliorated through antioxidant‐ inflammation‐related pathways; meanwhile, metabolites play a nonsubstitutable role process.

Язык: Английский

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Neonicotinoid and bisamide insecticides in wild freshwater fish from the Wanquan River, Hainan, China: Factors affecting tissue distribution and a risk assessment

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 126053 - 126053

Опубликована: Март 1, 2025

Язык: Английский

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