Frontiers in Chemical Biology,
Год журнала:
2024,
Номер
3
Опубликована: Дек. 20, 2024
Inflammation-related
disorders,
such
as
autoimmune
diseases
and
cancer,
impose
a
significant
global
health
burden.
Zinc
finger
proteins
(ZFs)
are
ubiquitous
metalloproteins
which
regulate
inflammation
many
biological
signaling
pathways
related
to
growth,
development,
immune
function.
Numerous
ZFs
involved
in
the
nuclear
factor
kappa-light-chain-enhancer
of
activated
B
cells
(NFκB)
pathway,
associating
them
with
inflammation-related
that
feature
chronically
elevated
pro-inflammatory
cytokines.
This
review
highlights
predominance
NFκB-related
summarizes
breadth
functions
these
perform.
The
cysteine-specific
post-translational
modification
(PTM)
persulfidation
is
also
discussed
context
cysteine-rich
ZFs,
including
what
known
from
few
available
reports
on
functional
implications
ZF
persulfidation.
Persulfidation,
mediated
by
endogenously
produced
hydrogen
sulfide
(H
2
S),
has
recently
established
role
inflammation.
work
will
summarize
connections
between
potential
inform
development
therapies.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(33)
Опубликована: Авг. 8, 2023
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
the
causative
agent
of
COVID-19,
uses
an
RNA-dependent
RNA
polymerase
along
with
several
accessory
factors
to
replicate
its
genome
and
transcribe
genes.
Nonstructural
protein
(nsp)
13
is
a
helicase
required
for
viral
replication.
Here,
we
found
that
nsp13
ligates
iron,
in
addition
zinc,
when
purified
anoxically.
Using
inductively
coupled
plasma
mass
spectrometry,
UV-visible
absorption,
EPR,
Mössbauer
spectroscopies,
characterized
as
iron–sulfur
(Fe–S)
Fe
4
S
cluster
treble-clef
metal-binding
site
zinc-binding
domain.
The
Fe–S
modulates
both
binding
template
unwinding
activity.
Exposure
stable
nitroxide
TEMPOL
oxidizes
degrades
drastically
diminishes
Thus,
optimal
function
depends
on
labile
potentially
targetable
COVID-19
treatment.
Abstract
Iron-sulfur
(Fe-S)
clusters
are
an
essential
and
ubiquitous
class
of
protein-bound
prosthetic
centers
that
involved
in
a
broad
range
biological
processes
(e.g.
respiration,
photosynthesis,
DNA
replication
repair
gene
regulation)
performing
wide
functions
including
electron
transfer,
enzyme
catalysis,
sensing.
In
general
manner,
Fe-S
can
gain
or
lose
electrons
through
redox
reactions,
highly
sensitive
to
oxidation,
notably
by
small
molecules
such
as
oxygen
nitric
oxide.
The
[2Fe-2S]
[4Fe-4S]
clusters,
the
most
common
cofactors,
typically
coordinated
four
amino
acid
side
chains
from
protein,
usually
cysteine
thiolates,
but
other
residues
histidine,
aspartic
acid)
also
be
found.
While
diversity
cluster
coordination
ensures
functional
variety
lack
conserved
motifs
makes
new
protein
identification
challenging
especially
when
is
shared
between
two
proteins
observed
several
dimeric
transcriptional
regulators
mitoribosome.
Thanks
recent
development
cellulo,
vitro,
silico
approaches,
still
regularly
identified,
highlighting
this
proteins.
review,
we
will
present
three
main
explain
difficulties
encountered
identify
methods
have
been
employed
overcome
these
issues.
This
year
marks
the
20th
anniversary
of
field
metallomics.
As
a
landmark
in
time,
it
is
an
occasion
to
reflect
on
past,
present,
and
future
this
integrated
biometal
sciences.
A
fundamental
bias
one
reason
for
having
metallomics
as
scientific
discipline.
The
focus
biochemistry
six
non-metal
chemical
elements,
collectively
known
with
acronym
SPONCH
(sulphur,
phosphorus,
oxygen,
nitrogen,
carbon,
hydrogen),
glosses
over
fact
that
lower
quantities
many
other
elements
have
qualities
made
them
instrumental
evolution
life
pivotal
numerous
processes.
metallome,
alongside
genome,
proteome,
lipidome,
glycome,
should
be
regarded
fifth
pillar
elemental-vis-à-vis
molecular-building
blocks
biochemistry.
Metallomics
'global
approaches
metals
biosciences'
considers
biological
significance
most
periodic
table,
not
only
ones
essential
life,
but
also
non-essential
are
present
living
matter-some
at
higher
concentrations
than
ones.
bioactive
either
positive
or
negative
effects.
Integrating
more
into
sciences
requires
transformation
learning
teaching
elemental
biology
addition
molecular
biology.
It
include
dynamic
interactions
between
biosphere
geosphere
how
human
footprint
changing
ecology
globally
exposing
us
additional
become
new
bioelements.
Inorganics,
Год журнала:
2024,
Номер
12(1), С. 34 - 34
Опубликована: Янв. 18, 2024
Viruses
rely
on
host
cells
to
replicate
their
genomes
and
assemble
new
viral
particles.
Thus,
they
have
evolved
intricate
mechanisms
exploit
factors.
Host
cells,
in
turn,
developed
strategies
inhibit
viruses,
resulting
a
nuanced
interplay
of
co-evolution
between
virus
host.
This
dynamic
often
involves
competition
for
resources
crucial
both
cell
survival
replication.
Iron
iron-containing
cofactors,
including
iron–sulfur
clusters,
are
known
be
heavily
fought
resource
during
bacterial
infections,
where
control
over
iron
can
tug
the
war
favor
pathogen
or
It
is
logical
assume
that
viruses
also
engage
this
competition.
Surprisingly,
our
knowledge
about
how
utilize
(Fe)
(FeS)
clusters
remains
limited.
The
handful
reviews
topic
primarily
emphasize
significance
supporting
immune
response
against
infections.
aim
review,
however,
organize
current
understanding
proteins
FeS
give
perspectives
what
questions
ask
next
propose
important
avenues
future
investigations.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(38)
Опубликована: Сен. 9, 2024
Modern
life
requires
many
different
metal
ions,
which
enable
diverse
biochemical
functions.
It
is
commonly
assumed
that
ions’
environmental
availabilities
controlled
the
evolution
of
early
life.
We
argue
can
only
explore
chemistry
encounters,
and
fortuitous
chemical
interactions
between
ions
biological
compounds
be
selected
for
if
they
first
occur
sufficiently
frequently.
calculated
maximal
transition
ion
concentrations
in
ancient
ocean,
determining
amounts
biologically
important
were
orders
magnitude
lower
than
ferrous
iron.
Under
such
conditions,
primitive
bioligands
would
predominantly
interact
with
Fe(II).
While
other
metals
certain
environments
may
have
provided
evolutionary
opportunities,
capacities
Fe(II),
Fe–S
clusters,
or
plentiful
magnesium
calcium
could
satisfied
all
functions
needed
by
Primitive
organisms
used
Fe(II)
exclusively
their
requirements.
