Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

и другие.

Phytochemical Analysis, Год журнала: 2024, Номер unknown

Опубликована: Сен. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Язык: Английский

Decoding the chemical material basis for detoxification and efficacy enhancement of aconite in Zhenwu Decoction using ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry and the Euclidean distance method DOI
Chongyang Wang,

Yourun Chen,

Kaiwen Zhang

и другие.

Microchemical Journal, Год журнала: 2025, Номер 213, С. 113569 - 113569

Опубликована: Апрель 12, 2025

Язык: Английский

Процитировано

0
Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

и другие.

Phytochemical Analysis, Год журнала: 2024, Номер unknown

Опубликована: Сен. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Язык: Английский

Процитировано

1