Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Language: Английский

Decoding the chemical material basis for detoxification and efficacy enhancement of aconite in Zhenwu Decoction using ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry and the Euclidean distance method DOI
Chongyang Wang,

Yourun Chen,

Kaiwen Zhang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: 213, P. 113569 - 113569

Published: April 12, 2025

Language: Английский

Citations

0
Investigation of bioactive components and metabolic pathways of Zhen‐wu‐tang in rat plasma and renal tissue by UPLC‐Q‐TOF/MS DOI

Shengliang Yuan,

Junqi Chen, Yiwen Cao

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 24, 2024

Abstract Introduction Zhen‐wu‐tang (ZWT) is a traditional Chinese medicine (TCM) formula for the treatment of several kidney diseases. However, due to complexity TCM formula, there lack accurate knowledge chemical constituents ZWT and its bioactive components, as well in vivo metabolic pathway studies. Objectives The composition components along with pathways were investigated by combination profiling serum pharmacochemistry. Methods High‐resolution ultra‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry was used identify metabolites vivo. Results As result, total 110 identified from solution, mainly amino acids, alkaloids, gingerols, monoterpene glycosides terpenoids, so on. In addition, 24 prototype 36 detected rat plasma. Meanwhile, 8 tissue but no metabolites. Interestingly, 4 28 both plasma renal tissue, which atractylenolide III, trimethoxyaconitane, methyl gallate, paeoniflorin. involved Phases I and/or II reactions such hydrolysis, oxidation, reduction hydration, methylation/demethylation, sulphation, glucuronidation, acetylation, glutathione conjugation. Conclusion Overall, present study has comprehensively elucidated potential metabolites, provides basis basic pharmacodynamic substances reference formulae.

Language: Английский

Citations

1