Characterisation of Castration-Resistant Cell-Derived Exosomes and Their Effect on the Metastatic Phenotype

Cancers, Год журнала: 2025, Номер 17(1), С. 141 - 141

Опубликована: Янв. 4, 2025

Background/Objectives: Prostate cancer (PCa) is characterised by its progression to a metastatic and castration-resistant phase. tumour cells release small extracellular vesicles or exosomes which are taken up target can potentially facilitate growth metastasis. The present work studies the effect of from cell lines that representative different stages disease on tumoral phenotype PC3 cells. Methods: Exosomes were isolated ultracentrifugation human prostate epithelial (RWPE-1) androgen-dependent PCa (LNCaP) (CRPC) with moderate (DU145) high (PC3) capacity. biophysical biochemical properties as well their effects viability migration. Results: study shows heterogeneity in size, presenting some them two types populations; both populations, larger size those derived smaller non-tumourigenic detected. Differences found physical healthy cells, between most aggressive disease. highest gamma-glutamyl transferase (GGT) activity was observed differences pro-metalloproteinases (MMP) detected capacity respect treatment own increased Conclusion: represent promising field research diagnosis, prognosis, cancer.

Язык: Английский

---

Effect of Molecular Weight of Fluorescent Dyes on DNA Separation by Capillary Electrophoresis

Journal of Fluorescence, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Язык: Английский

---

Identity confirmation of extracellular vesicles by capillary electrophoresis using non-specific dyes

Microchemical Journal, Год журнала: 2024, Номер 207, С. 112288 - 112288

Опубликована: Ноя. 28, 2024

Язык: Английский

---

On-line sample pre-concentration in capillary electrophoresis: Fundamentals and computational simulation

Elsevier eBooks, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

---