Characterisation of Castration-Resistant Cell-Derived Exosomes and Their Effect on the Metastatic Phenotype

Cancers, Journal Year: 2025, Volume and Issue: 17(1), P. 141 - 141

Published: Jan. 4, 2025

Background/Objectives: Prostate cancer (PCa) is characterised by its progression to a metastatic and castration-resistant phase. tumour cells release small extracellular vesicles or exosomes which are taken up target can potentially facilitate growth metastasis. The present work studies the effect of from cell lines that representative different stages disease on tumoral phenotype PC3 cells. Methods: Exosomes were isolated ultracentrifugation human prostate epithelial (RWPE-1) androgen-dependent PCa (LNCaP) (CRPC) with moderate (DU145) high (PC3) capacity. biophysical biochemical properties as well their effects viability migration. Results: study shows heterogeneity in size, presenting some them two types populations; both populations, larger size those derived smaller non-tumourigenic detected. Differences found physical healthy cells, between most aggressive disease. highest gamma-glutamyl transferase (GGT) activity was observed differences pro-metalloproteinases (MMP) detected capacity respect treatment own increased Conclusion: represent promising field research diagnosis, prognosis, cancer.

Language: Английский

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Effect of Molecular Weight of Fluorescent Dyes on DNA Separation by Capillary Electrophoresis

Journal of Fluorescence, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Language: Английский

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Identity confirmation of extracellular vesicles by capillary electrophoresis using non-specific dyes

Microchemical Journal, Journal Year: 2024, Volume and Issue: 207, P. 112288 - 112288

Published: Nov. 28, 2024

Language: Английский

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On-line sample pre-concentration in capillary electrophoresis: Fundamentals and computational simulation

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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