Methods in molecular biology, Год журнала: 2024, Номер unknown, С. 355 - 368
Опубликована: Дек. 23, 2024
Язык: Английский
Nature reviews. Immunology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 9, 2024
Язык: Английский
Процитировано
34
Chemical Reviews, Год журнала: 2024, Номер 124(20), С. 11242 - 11347
Опубликована: Окт. 9, 2024
Biopsy, including tissue and liquid biopsy, offers comprehensive real-time physiological pathological information for disease detection, diagnosis, monitoring. Fluorescent probes are frequently selected to obtain adequate on processes in a rapid minimally invasive manner based their advantages biopsy. However, conventional fluorescent have been found show aggregation-caused quenching (ACQ) properties, impeding greater progresses this area. Since the discovery of aggregation-induced emission luminogen (AIEgen) promoted advancements molecular bionanomaterials owing unique high quantum yield (QY) signal-to-noise ratio (SNR),
Язык: Английский
Процитировано
22
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 2, 2025
Different forms of phosphorylated tau (p-tau) have shown strong potential as Alzheimer's disease (AD) biomarkers in both cerebrospinal fluid (CSF) and plasma. We hypothesized that p-tau proteoforms simultaneously at two different sites may an increased diagnostic value compared with a single site. Here, we developed immunoassays detecting CSF plasma T181 T231 (p-tau181&231) T217 (p-tau217&231). Subsequently, measured p-tau181&231, p-tau217&231, p-tau181, p-tau217, p-tau231 levels cohorts across the AD continuum frontotemporal dementia (FTD) patients (discovery n = 55, validation 118). CSF, but not plasma, p-tau181&231 were significantly elevated all groups vs. Neurological Disease Control group. Notably, p-tau217&231 consistently showed improved performance single-site phosphorylation assays – p-tau217 or p-tau231. The differences observed between measurements suggest matrix-specific protein processing, underscoring need for further research on dynamics pattern along continuum. Cerebrospinal distinct (p-tau181&231 p-tau217&231) provide high accuracy biomarkers. In similar was obtained only p-tau217&231.
Язык: Английский
Процитировано
3
The Lancet Regional Health - Europe, Год журнала: 2024, Номер 44, С. 101009 - 101009
Опубликована: Авг. 23, 2024
Multiple sclerosis (MS) is an immune-mediated inflammatory and degenerative disorder of the central nervous system (CNS) with heterogeneous clinical manifestations. In last decade, landscape cerebrospinal fluid (CSF) blood biomarkers as potential key tools for MS diagnosis, prognosis treatment monitoring has evolved considerably, alongside magnetic resonance imaging (MRI). CSF analysis not only to provide information on underlying immunopathology disease exclude differential diagnoses, but also predict risk future relapses disability accrual, guide therapeutic decisions thus improve patient outcomes. This Series article overviews biological framework current applicability MS, exploring their role in molecular characterisation disease. We discuss recent advances field neurochemistry that enabled detection brain-derived proteins blood, opening door much more efficient longitudinal monitoring. Furthermore, we identify challenges application a real-world setting, while offering recommendations harnessing full paraclinical management personalise treatment.
Язык: Английский
Процитировано
16
Alcohol research, Год журнала: 2024, Номер 44(1)
Опубликована: Янв. 1, 2024
PURPOSE:By 2040, 21.6% of Americans will be over age 65, and the population those older than 85 is estimated to reach 14.4 million.Although not causative, a risk factor for dementia: every 5 years beyond doubles; approximately one-third are diagnosed with dementia.As current alcohol consumption among adults significantly higher compared previous generations, pressing question whether drinking increases Alzheimer's disease or other forms dementia.SEARCH METHODS: Databases explored included PubMed, Web Science, ScienceDirect.To accomplish this narrative review on effects dementia risk, literature covered clinical diagnoses, epidemiology, neuropsychology, postmortem pathology, neuroimaging biomarkers, translational studies.Searches conducted between January 12 August 1, 2023, following terms combinations: "aging," "alcoholism," "alcohol use disorder (AUD)," "brain," "CNS," "dementia," "Wernicke," "Korsakoff," "Alzheimer," "vascular," "frontotemporal," "Lewy body," "clinical," "diagnosis," "epidemiology," "pathology," "autopsy," "postmortem," "histology," "cognitive," "motor," "neuropsychological," "magnetic resonance," "imaging," "PET," "ligand," "degeneration," "atrophy," "translational," "rodent," "rat," "mouse," "model," "amyloid," "neurofibrillary tangles," "α-synuclein," "presenilin."When relevant, "species" (i.e., "humans" "other animals") was selected as an additional filter.Review articles were avoided when possible.SEARCH RESULTS: The two "alcoholism" "aging" retrieved about 1,350 papers; adding phrases-for example, "postmortem" resonance"-limited number fewer 100 papers.Using traditional term, "dementia" resulted in 876 citations, but using currently accepted term (AUD)" produced only 87 papers.Similarly, whereas "Alzheimer's" yielded 318 results, returned 40 citations.As pertinent pathology papers published 1950s recent animal models created early 2000s, referenced span 1957 2024.In total, more 5,000 considered; 400 herein referenced.DISCUSSION AND CONCLUSIONS: Chronic misuse accelerates brain aging contributes cognitive impairments, including mnemonic domain.The consensus studies from multiple disciplines, however, that can increase dementia, necessarily disease.Key issues consider include reversibility damage abstinence chronic degenerative progressive course disease, characteristic presence protein inclusions brains people which absent AUD.
Язык: Английский
Процитировано
9
Alzheimer s Research & Therapy, Год журнала: 2024, Номер 16(1)
Опубликована: Авг. 19, 2024
Plasma p-tau217 has emerged as the most promising blood-based marker (BBM) for detection of Alzheimer Disease (AD) pathology, yet few studies have evaluated plasma performance in memory clinic settings. We examined AD using a high-sensitivity immunoassay individuals undergoing diagnostic lumbar puncture (LP).
Язык: Английский
Процитировано
6
Antioxidants, Год журнала: 2024, Номер 13(6), С. 681 - 681
Опубликована: Май 31, 2024
Oxidation–reduction post-translational modifications (redox-PTMs) are chemical alterations to amino acids of proteins. Redox-PTMs participate in the regulation protein conformation, localization and function, acting as signalling effectors that impact many essential biochemical processes cells. Crucially, dysregulation redox-PTMs proteins has been implicated pathophysiology numerous human diseases, including neurodegenerative diseases such Alzheimer’s disease Parkinson’s disease. This review aims highlight current gaps knowledge field biology explore new methodological advances proteomics computational modelling will pave way for a better understanding role therapeutic potential diseases. Here, we summarize main types while providing examples their occurrence an overview state-of-the-art methods used detection. We novel approaches tools obtain insights into precise regulating structure function. also discuss complex crosstalk between various PTMs occur living Finally, argue could be future diagnosis prognosis biomarkers
Язык: Английский
Процитировано
5
The Journal of Prevention of Alzheimer s Disease, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
4
Molecular & Cellular Proteomics, Год журнала: 2024, Номер 23(2), С. 100688 - 100688
Опубликована: Янв. 27, 2024
Methods to Discover and Validate Biofluid-Based Biomarkers in Neurodegenerative DementiasMolecular & Cellular ProteomicsVol. 22Issue 10PreviewIn Brief We review technologies used for body fluid protein biomarker discovery translation clinical practice. address their main characteristics, use research clinics, strengths limitations, a future perspective. Full-Text PDF Open AccessThe First Pituitary Proteome Landscape From Matched Anterior Posterior Lobes Better Understanding of the GlandMolecular 1PreviewIn Our study first pituitary proteome draft offers following novelty; time, we present an extensive proteomic database containing proteins from both anterior posterior lobes healthy pituitary, identifying various pituitary-enriched lobe specificities, followed by target verification hormones; are describing three uPE1 have demonstrated that S100 proteins, which known markers adenomas, showed presence significantly lobe. AccessSARS-CoV-2 Variants Show Different Host Cell Profiles With Delayed Immune Response Activation Omicron-Infected CellsMolecular 5PreviewIn SARS-CoV-2 has mutated over past years numerous variants concern (VOCs). Employing quantitative whole-cell proteomics, elucidate host cell immune responses upon infection with ancestral B.1, VOCs Delta, Omicron BA.1 strains. Molecular assays further illustrate reduced viral levels delayed pathway response when compared B.1 Delta. Overall, this insights into profiles distinct kinetics AccessProteomic Characterization Acute Myeloid Leukemia Precision MedicineMolecular 4PreviewIn Proteomic genomic studies identified new drug targets acute myeloid leukemia (AML), leading therapeutic options certain patient subpopulations. In addition, many other drugs advanced stages development proteomics keeps uncovering interest. Given large number therapies likely be available AML near future, identification signatures each will key select best treatment given patient. AccessSensitive, High-Throughput HLA-I HLA-II Immunopeptidomics Using Parallel Accumulation-Serial Fragmentation Mass SpectrometryMolecular 6PreviewIn In-depth mass spectrometry–based profiling human leukocyte antigen (HLA) peptides currently requires amounts sample, off-line fractionation is frequently improve coverage. However, primary tissues often input limited therefore not amenable such workflows. Here, developed optimized single-shot acquisition strategy on timsTOF single-cell sensitive high-throughput immunopeptidome analysis. demonstrate >2-fold increase identfied propose multiple collision energy slopes samples different peptide-binding motifs. AccessProspective Imaging Spectrometry Clinical DiagnosticsMolecular 9PreviewIn spectrometry emerging technology applications it provides spatial molecular data within tissue biopsy diagnoses prognoses. This article considers aspects imaging-based include analyte selection, quality control/assurance metrics, reproducibility, classification, scoring. AccessCross-Linking Uncovers Interactions Between High-Density Lipoproteins Spike GlycoproteinMolecular 8PreviewIn Here utilized cross-linking (XL-MS) investigate circulating interaction networks context SARS-CoV-2. The spike glycoprotein was determined interact high-density lipoprotein including ApoD. between ApoD confirmed cells through affinity purification-MS. XL-MS recombinant ApoD, coupled structural modeling, receptor-binding domain. overexpression cultured lines did influence replication or infection. AccessUncovering Protein Networks Cardiovascular ProteomicsMolecular article, critically assess existing methods reconstructing discuss method preferred cardiovascular research. necessity reconstruct separately type disease entity provide illustrative examples importance taking consideration relevant post-translational modifications. Finally, how findings could interpreted using RNA-sequencing data. AccessFunctional Impact Protein–RNA Variation Cancer AnalysesMolecular 7PreviewIn Proteogenomic cancer highlight broad protein–RNA discrepancies. Tumor Analysis Consortium (CPTAC) mRNA eight indications, show impact low RNA–protein correlations biological processes, signaling pathways, targets. lead suggesting protein-based subtyping may unravel features seen RNA measurements. These analyses critical role phenotypic tumor characterization. AccessMass Spectrometry–Based Proteomics Epithelial Ovarian Cancers: A PerspectiveMolecular systematically reviewed MS-based epithelial ovarian cancer, 2000 trials since 1990. None been adopted clinic, none firstly discovered phase 3 4. Stringent standards design required, more in-depth dysregulated essential. AccessClinical Solid Organ TissuesMolecular 11PreviewIn For years, immunohistochemistry tool pathology localization quantification solid tissues, but poorly standardized suffers practical limitations. field ripe novel complementary help diagnosis. perspective focuses issues hampering highlights liquid chromatography-tandem particular well-positioned complement testing. also where guidance needed ensure rigor environments. AccessIntegrated Understand Role Neuritin (NRN1) as Mediator Cognitive Resilience Alzheimer's DiseaseMolecular Mechanisms individuals (AD) avoid dementia well known. employed multiplex tandem tag (TMT-MS)–based identify linked cognitive resilience AD. Computational strategies NRN1 synaptic associated resilience. models AD, can facilitate dendritic spine against amyloid-β (Aβ) block Aβ-induced neuronal hyperexcitability. assessed alters TMT-MS, revealed overlapping synapse-related biology humans. Exploration Pancreatic CancerMolecular extremely lethal options. Identifying effective early-detection biomarkers urgently needed. review, describe recent advances functional pancreatic cancer. Regarding modifications–mediated intracellular signaling, ligands plasma membrane receptors–mediated intercellular signaling. terms bulk diagnostic studying heterogeneity. AccessMulti-Omics Profiling HealthMolecular Current medical care treating people after they become patients rather than preventing illness, high costs chronic late-stage diseases. Additionally, "one-size-fits all" approach healthcare does take account individual differences genetics, environment, lifestyle factors, decreasing benefiting interventions. Rapid multi-omics enabled deep phenotyping, empowers precision health approaches poised transform healthcare. AccessTen Years Extracellular Matrix Proteomics: Accomplishments, Challenges, Future PerspectivesMolecular extracellular matrix (ECM) complex assembly hundreds forming architectural organizer multicellular organisms. Over decade, bottom-up choice profile composition ECM. reviews state art ECM illustrates emerged powerful pipeline biomarkers. introduces resources proteomics. AccessUltrasensitive Detection Technologies Vesicle MeasurementsMolecular To harness full potential vesicles (EVs) applications, necessary find appropriate enable selective isolation rare subpopulations EVs. Many conventional detection sensitivity detect abundance EVs, limiting EV perspective, current ultrasensitive technologies, areas growth future. Glyco(proteo)mics increasingly mature toolbox glycomic- glycoproteomic applied biopharmaceuticals evaluation glycobiomarkers fields. glycoproteomics, parallel made earlier proposed tiers spectrometry. Multimarker readouts foreseen longitudinal sampling monitoring glycomic diagnosis monitoring. accurate multimarker panel discussed. AccessProteome Mapping Human Islet Microenvironment Reveals Endocrine–Exocrine Signaling Sphere InfluenceMolecular our Microdroplet Processing One pot Trace Samples (microPOTS) TMT platform islet microenvironment pancreas. were able quantify 6000 seven regions same enhancements computational extract hypotheses first-of-its-kind dataset. addition recapitulating functions islet, specific processing activities heterogeneity expression. AccessPlasma Kinetics ExerciseMolecular After measuring 4163 before, during, 1-h treadmill exercise 75 middle-aged adults, found 765 changed at peak exercise, majority these returned baseline after. Of 56 sustained change rest, there enrichment secreted association cardiometabolic traits independent cohort, promising candidates investigation. AccessDissecting Detergent-Insoluble Disease TMTc-Corrected Quantitative TMT-LC/LC–MS/MS TMTc-based correct ratio compression, analyzed detergent-insoluble 30 AD control brain samples. 84 enriched fraction, accumulated mostly low-complexity regions. pathological hallmarks resistant detergent extraction, providing list pathways exclusively proteome. AccessMS-Based Body Fluids: End BeginningMolecular its nature suited routine measurement held back technological conceptual constraints. improvements throughput, streamlining, robustness, progressively overcoming constraints recently success stories. Cutting-edge scan modes, multiplexing, sample preparation promise enhance technology, envision finally bring clinic. AccessAn Inflection Point Biomarkers: What What's NextMolecular highest value proposition medicine today. Despite progress sciences evolving regulatory frameworks biomarkers, about improving health. Achieving necessitates moving away reductionist thinking diseases adaptive systems defining representations system states. redefining communication, ultimately prove game changing who benefit. Atlas Knee Joint Proteins Their Osteoarthritis Defined Literature MiningMolecular Information gathered analysis knee joint essential management osteoarthritis, most prevalent rheumatic pathology. Pursuing one goals Project, systematic based literature mining define atlas organ, prioritization cited representative tissues. Pathway depicted processes contributing OA pathophysiology. AccessProteomics: Progress Promise Chronic Kidney Population genetics leverage large-scale accuracy tools kidney disease. Access "Clinical proteomics" understood encompass spectrum activity pre-clinical diagnostics: clinically materials; addressing question need; (MS)-based proteomics-derived tests reference laboratory informing decision-making. widely explore basis disease, targetable intervention, predict outcome response, probe resistance mechanisms. special issue presents wide range research, reviews, perspectives pertaining expanding scope reflected foci represented, neurodegenerative (1Teunissen C.E. Kimble L. Bayoumy S. Bolsewig K. Burtscher F. Coppens et al.MIRIADE consortium. discover validate biofluid-based dementias.Mol. Proteomics. 2023; 22100629Abstract Full Text Scopus (3) Google Scholar, 2Gobom J. Brinkmalm A. G. Blennow Zetterberg H. targeted spectrometry.Mol. 2024; 23 (100721)Google 3Hurst C. Pugh D.A. Abreha M.H. Duong D.M. Dammer E.B. Bennett al.Integrated understand neuritin mediator disease.Mol. 22100542Abstract (2) 4Zaman M. Fu Y. Chen P.C. Sun Yang Wu Z. al.Dissecting TMTc-corrected 22100608Abstract Scholar), infectious (5Burnap S.A. Ortega-Prieto A.M. Jimenez-Guardeño J.M. Ali Takov Fish al.Cross-linking uncovers interactions lipoproteins glycoprotein.Mol. 22100600Abstract (0) 6Metzler Tharyan R.G. Klann Grikscheit Bojkova D. Cinatl al.SARS-CoV-2 activation omicron-infected cells.Mol. 22100537Abstract (1) endocrinology (7Banerjee Biswas Barpanda Halder Sibal Kattimani R. al.The landscape matched better understanding gland.Mol. 22100478Abstract PubMed 8Gosline S.J.C. Veličković Pino J.C. Day L.Z. Attah I.K. Swensen A.C. al.Proteome mapping reveals endocrine-exocrine sphere influence.Mol. 22100592Abstract (9Hasman Mayr Theofilatos Uncovering proteomics.Mol. 22100607Abstract inflammatory (10Paz-González Lourido Calamia V. Fernández-Puente P. Quaranta Picchi al.An osteoarthritis defined mining.Mol. 22100606Abstract (11Schlosser Grams M.E. Rhee E.P. 22100550Abstract (12Huang Gao W. Tian Functional exploration cancer.Mol. 22100575Abstract 13Casado Cutillas P.R. characterization medicine.Mol. 22100517Abstract (4) 14Arad Geiger T. protein-RNA variation analyses.Mol. 22100587Abstract 15Qian Xue Guo spectrometry-based cancers: perspective.Mol. 22100578Abstract burgeoning (16Mi M.Y. Barber J.L. Rao Farrell L.A. Sarzynski M.A. Bouchard al.Plasma exercise.Mol. 22100601Abstract 17Babu Snyder Multi-omics health.Mol. 22100561Abstract (13) Scholar). part instrumentation, methodologies, capabilities. Methodological allowed detailed challenging materials like (18Naba Ten proteomics: accomplishments, challenges, perspectives.Mol. 22100528Abstract Scholar) CNS (4Zaman (EV) payloads driven robust (19Shami-Shah Norman Walt D.R. Ultrasensitive vesicle measurements.Mol. 22100557Abstract immunopeptidomics inform immunological guide vaccine expanded owing optimization (20Phulphagar K.M. Ctortecka Jacome A.S.V. Klaeger Verzani E.K. Hernandez G.M. al.Sensitive, accumulation-serial fragmentation 22100563Abstract remains workhorse contemporary scale array-based make them attractive alternative 16Mi Complex cross-sectional, multi-omic facilitated accelerating computer speeds, storage capabilities, promulgation focused (17Babu 18Naba Improved sample-handling instrument contributed expansion spatially-resolved (8Gosline Prior limitations prevented numbers being greatly highly multiplexed (3Hurst far MS systems, scanning acquisition. Targeted reaction application modifications assessing stage effects drugs. largely purposes candidate preclinical measure peptide PTM-modified related imaging explored augment replace histopathologic suite (21Moore Patterson N.H. Norris Caprioli R.M. Prospective diagnostics.Mol. 22100576Abstract 22Phipps W.S. Kilgore M.R. Kennedy J.J. Whiteaker J.R. Hoofnagle A.N. Paulovich A.G. organ tissues.Mol. 22100648Abstract especially important deepening perturbations posttranslational phosphorylation, ubiquitylation, acetylation, sumoylation, glycosylation. now PTMs scale, individually serial yielding global exactly thereby facilitating integration while sparing precious material. Such comprehensive portraits much alone. Bottlenecks lack access annotation follow-up information integrated slowly proteogenomics enhancing becomes ever clearer (23Mani Krug Zhang B. Satpathy Clauser K.R. Ding al.Cancer proteogenomics: prospects.Nat. Rev. Cancer. 2022; 22: 298-313Crossref (56) While initially overhyped, considerable evidence techniques depth, reproducibility required aid diagnosis, prognosis, clinically-actionable prediction 24Bader Albrecht Mann fluids: end beginning.Mol. 22100577Abstract (5) 25Barker A.D. Alba M.M. Mallick Agus D.B. Lee J.S.H. An inflection point biomarkers: what what's next.Mol. 22100569Abstract 26van der Burgt Wuhrer glyco(proteo)mics 22100565Abstract needs additional validation, assay refinement, economics assessment, viable reimbursement remain challenges deployment problems clinic works progress, papers demonstrate, greater ever, likelihood having direct, positive interventions precise therapies. member scientific advisory boards Kymera, PTM BioLabs, Seer. G members board PrognomIQ. work supported grants P01CA206978 S.A.C. NIH, U24CA270823 U01CA271402 C U24-CA271075 National Institute (NCI) program, Swiss Science Foundation (SNF) grant CRSII5_186405 C., Dr Miriam Sheldon Adelson Medical Research thank entire Broad group valuable discussions.
Язык: Английский
Процитировано
3
Journal of Clinical Neuroscience, Год журнала: 2024, Номер 126, С. 119 - 127
Опубликована: Июнь 12, 2024
Язык: Английский
Процитировано
3