Science Signaling,
Год журнала:
2015,
Номер
8(408)
Опубликована: Дек. 22, 2015
The
Ca(2+)
release-activated
channel
mediates
influx
in
a
plethora
of
cell
types,
thereby
controlling
diverse
cellular
functions.
complex
is
composed
stromal
interaction
molecule
1
(STIM1),
an
endoplasmic
reticulum
Ca(2+)-sensing
protein,
and
Orai1,
plasma
membrane
channel.
Channels
STIM1
Orai1
mediate
even
at
low
extracellular
concentrations.
We
investigated
whether
the
activity
adapted
to
different
environmental
used
homology
modeling
molecular
dynamics
simulations
predict
presence
Ca(2+)-accumulating
region
(CAR)
pore
entrance
Orai1.
Furthermore,
proteins
with
mutations
CAR,
along
live-cell
experiments,
or
electrophysiological
recordings
transient,
electrostatic
loop3
interacting
loop1
(the
site
CAR)
determined
that
CAR
enhanced
permeation
most
efficiently
external
Consistent
these
results,
cells
expressing
mutants
exhibited
impaired
gene
expression
stimulated
by
Ca(2+)-activated
transcription
factor
nuclear
activated
T
(NFAT).
propose
architecture
close
proximity
selectivity
filter,
which
enables
Ca(2+)-selective
ion
permeation,
enhances
local
concentration
maintain
Ca(2+)-dependent
regulation
environments
relatively
Ca(2+)concentrations.
F1000Research,
Год журнала:
2018,
Номер
7, С. 260 - 260
Опубликована: Март 2, 2018
Nuclear
factor
of
activated
T
cells
(NFAT)
was
first
described
almost
three
decades
ago
as
a
Ca2+/calcineurin-regulated
transcription
in
cells.
Since
then,
large
body
research
uncovered
the
regulation
and
physiological
function
different
NFAT
homologues
immune
system
many
other
tissues.
In
this
review,
we
will
discuss
novel
roles
cells,
focusing
mainly
on
its
humoral
responses,
immunological
tolerance,
metabolism.
AJP Cell Physiology,
Год журнала:
2016,
Номер
310(8), С. C643 - C662
Опубликована: Янв. 30, 2016
Ca(2+)entry
into
the
cell
via
store-operated
Ca(2+)release-activated
Ca(2+)(CRAC)
channels
triggers
diverse
signaling
cascades
that
affect
cellular
processes
like
growth,
gene
regulation,
secretion,
and
death.
These
Ca(2+)channels
open
after
depletion
of
intracellular
Ca(2+)stores,
their
main
features
are
fully
reconstituted
by
two
molecular
key
players:
stromal
interaction
molecule
(STIM)
Orai.
STIM
represents
an
endoplasmic
reticulum-located
Ca(2+)sensor,
while
Orai
forms
a
highly
Ca(2+)-selective
ion
channel
in
plasma
membrane.
Functional
as
well
mutagenesis
studies
together
with
structural
insights
about
proteins
provide
picture
interplay
these
players
CRAC
cascade.
This
review
focuses
on
experimental
advances
understanding
STIM1-Orai
choreography,
thereby
establishing
portrait
mechanistic
steps
The
focus
is
activation
proteins,
subsequent
coupling
STIM1
to
Orai1,
consequent
rearrangements
gate
state
allow
Ca(2+)permeation
cell.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2019,
Номер
11(8), С. a035204 - a035204
Опубликована: Май 14, 2019
Processes
that
are
important
in
cancer
progression,
such
as
sustained
cell
growth,
invasion
to
other
organs,
and
resistance
death
inducers,
have
a
clear
overlap
with
pathways
regulated
by
Ca2+
signaling.
It
is
therefore
not
surprising
proteins
signaling,
sometimes
referred
the
"Ca2+
signaling
toolkit,"
can
contribute
proliferation
invasiveness,
ability
of
agents
induce
death.
also
critical
aspects
including
events
tumor
microenvironment
processes
involved
acquisition
anticancer
therapies.
This
review
will
consider
role
progression
highlight
areas
which
better
understanding
interplay
between
Ca2+-signaling
toolkit
tumorigenesis
still
required.
