Materials Today Bio,
Год журнала:
2023,
Номер
23, С. 100875 - 100875
Опубликована: Ноя. 19, 2023
Complete
and
rapid
healing
of
infected
skin
wounds
remains
a
challenge
in
current
clinical
treatment.
In
this
study,
we
prepared
self-healing
injectable
CK
hydrogel
by
crosslinking
two
natural
polysaccharides,
carboxymethyl
chitosan
oxidized
konjac
glucomannan,
based
on
the
Schiff
base
bond.
To
enhance
biological
function
hydrogel,
multi-functionalized
hydrogen
loading
it
with
berberine
(BBR)
stem
cell-derived
exosomes
(Exo),
forming
composite
CK@BBR&Exo,
which
could
be
injected
directly
into
wound
through
needle
adhered
to
wound.
Furthermore,
properties
CK@BBR&Exo
increased
its
usefulness
service
life.
Additionally,
drug-loaded
was
versatile,
inhibiting
bacterial
growth,
regulating
inflammatory
response,
promoting
neovascularization
wounds,
thus
achieving
wounds.
These
results
suggest
that
CK@BBR&Exo-injectable
is
an
ideal
dressing
for
treating
Stem Cell Research & Therapy,
Год журнала:
2023,
Номер
14(1)
Опубликована: Апрель 26, 2023
Abstract
Wound
healing
is
a
dynamic
and
highly
sequential
process
involving
series
of
overlapping
spatial
temporal
phases,
including
hemostasis,
inflammation,
proliferation,
tissue
remodeling.
Mesenchymal
stem
cells
(MSCs)
are
multipotent
with
self-renewal,
multidirectional
differentiation
potential,
paracrine
regulation.
Exosomes
subcellular
vesicular
components
30–150
nm
in
size
novel
carriers
intercellular
communication
regulating
the
biological
behaviors
skin
cells.
Compared
to
MSCs,
MSC-derived
exosomes
(MSC-exos)
possess
lower
immunogenicity,
easy
storage,
effective
activity.
MSC-exos,
mainly
derived
from
adipose-derived
(ADSCs),
bone
marrow-derived
MSCs
(BMSCs),
human
umbilical
cord
(hUC-MSCs),
other
cell
types,
play
role
shaping
activity
fibroblasts,
keratinocytes,
immune
cells,
endothelial
diabetic
wounds,
inflammatory
wound
repair,
even
wound-related
keloid
formation.
Therefore,
this
study
focuses
on
specific
roles
mechanisms
different
MSC-exos
healing,
as
well
current
limitations
various
perspectives.
Deciphering
properties
crucial
providing
promising
cell-free
therapeutic
tool
for
cutaneous
regeneration.
Graphical
Wound
healing
is
an
urgent
clinical
challenge,
particularly
in
the
case
of
chronic
wounds.
Traditional
approaches
to
wound
have
limited
therapeutic
efficacy
due
lengthy
times,
risk
immune
rejection,
and
susceptibility
infection.
Recently,
adipose-derived
mesenchymal
stem
cell-derived
exosomes
(ADSC-exos)
emerged
as
a
promising
modality
for
tissue
regeneration
repair.
In
this
study,
development
novel
extracellular
matrix
hydrogel@exosomes
(ECM@exo)
reported,
which
entails
incorporation
ADSC-exos
into
hydrogel
(ECM
hydrogel).
This
solution
forms
at
physiological
temperature
(≈37
°C)
upon
local
injection
site.
ECM@exo
enables
sustained
release
from
ECM
hydrogel,
maintains
high
concentrations
The
displays
good
biocompatibility
biodegradability.
vivo
vitro
results
demonstrate
that
treatment
effectively
reduces
inflammation
promotes
angiogenesis,
collagen
deposition,
cell
proliferation,
migration,
thereby
accelerating
process.
Overall,
innovative
approach
offers
new
avenue
via
biological
with
controlled
exosome
release.
International Journal of Nanomedicine,
Год журнала:
2023,
Номер
Volume 18, С. 4751 - 4778
Опубликована: Авг. 1, 2023
Abstract:
Exosomes,
as
natural
nanocarriers,
characterized
with
low
immunogenicity,
non-cytotoxicity
and
targeted
delivery
capability,
which
have
advantages
over
synthetic
nanocarriers.
Recently,
exosomes
shown
great
potential
diagnostic
markers
for
diseases
are
also
considered
a
promising
cell-free
therapy.
Engineered
significantly
enhanced
the
efficacy
precision
of
delivering
therapeutic
agents,
currently
being
extensively
employed
in
investigations
various
ailments,
including
oncology,
inflammatory
disorders,
degenerative
conditions.
Particularly,
engineered
enable
agent
loading,
modification,
evasion
MPS
phagocytosis,
intelligent
control,
bioimaging,
been
developed
multifunctional
nano-delivery
platforms
recent
years.
The
utilization
bioactive
scaffolds
that
loaded
exosome
has
to
substantially
augment
retention,
extend
release,
enhance
efficacy.
This
approach
advanced
from
conventional
hydrogels
nanocomposite
hydrogels,
nanofiber
3D
printing,
resulting
superior
physical
biological
properties
effectively
address
limitations
scaffolds.
Additionally,
plant-derived
exosomes,
can
participate
gut
flora
remodeling
via
oral
administration,
an
ideal
platform
treatment
intestinal
diseases.
Consequently,
there
is
interest
nanocarriers
applications.
comprehensive
review
provides
overview
biogenesis,
composition,
isolation
methods
exosomes.
it
examines
pathological
mechanisms
diseases,
tumors,
Furthermore,
this
highlights
significance
microbial-derived
Strategies
specific
applications
scaffold-loaded
further
summarized,
especially
some
new
techniques
such
large-scale
loading
technique,
macromolecular
development
nano-scaffold-loaded
delivery.
benefits
using
gut-related
discussed.
challenges,
opportunities,
prospects
exosome-based
disease
diagnosis
summarized
both
preclinical
clinical
viewpoints.
Keywords:
diagnosis,
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(4), С. 3888 - 3888
Опубликована: Фев. 15, 2023
Adipose-derived
mesenchymal
stromal
cells
(AD-MSCs)
have
been
extensively
studied
in
recent
years.
Their
attractiveness
is
due
to
the
ease
of
obtaining
clinical
material
(fat
tissue,
lipoaspirate)
and
relatively
large
number
AD-MSCs
present
adipose
tissue.
In
addition,
possess
a
high
regenerative
potential
immunomodulatory
activities.
Therefore,
great
stem
cell-based
therapies
wound
healing
as
well
orthopedic,
cardiovascular,
or
autoimmune
diseases.
There
are
many
ongoing
trials
on
AD-MSC
cases
their
effectiveness
has
proven.
this
article,
we
current
knowledge
about
based
our
experience
other
authors.
We
also
demonstrate
application
selected
pre-clinical
models
studies.
can
be
pillar
next
generation
that
will
chemically
genetically
modified.
Despite
much
research
these
cells,
there
still
important
interesting
areas
explore.
International Journal of Oral Science,
Год журнала:
2024,
Номер
16(1)
Опубликована: Апрель 16, 2024
Abstract
Bacterial
resistance
and
excessive
inflammation
are
common
issues
that
hinder
wound
healing.
Antimicrobial
peptides
(AMPs)
offer
a
promising
versatile
antibacterial
option
compared
to
traditional
antibiotics,
with
additional
anti-inflammatory
properties.
However,
the
applications
of
AMPs
limited
by
their
antimicrobial
effects
stability
against
bacterial
degradation.
TFNAs
regarded
as
drug
delivery
platform
could
enhance
properties
nanodrugs.
Therefore,
in
this
study,
composite
hydrogel
(HAMA/t-GL13K)
was
prepared
via
photocross-linking
method,
which
tFNAs
carry
GL13K.
The
injectable,
biocompatible,
be
instantly
photocured.
It
exhibited
broad-spectrum
inhibiting
expression
inflammatory
factors
scavenging
ROS.
Thereby,
inhibited
infection,
shortened
healing
time
skin
defects
infected
full-thickness
defect
models
reduced
scarring.
constructed
HAMA/tFNA-AMPs
hydrogels
exhibit
potential
for
clinical
use
treating
microbial
infections
promoting
