Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

и другие.

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Апрель 22, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) results in good diastolic and capacities, its altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs exist a synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense detecting changes crowding. However, unclear whether macromolecular crowding (MMC) helps phenotype. Purpose This study aimed explore phenotypic, behavioral gene expression after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay; survival activity were calcein/PI cell toxicity CCK-8 assays; phenotypes migration WB wound healing transcriptomic analysis RT-qPCR. Results Notably, 225 μg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic marker VSMCs, shortened length, decreased proliferation, inhibited migration. VSMCs. Specifically, 856 genes upregulated 1207 downregulated. These alterations primarily cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton processes whereas downregulated mainly Conclusions showed can sensing culture environment, which be maintained CR. Graphical

Язык: Английский

Underestimated role of macromolecular crowding in bioengineered in vitro models of health and diseases DOI Creative Commons
Jérémy Lagrange,

G. Velde,

Patrick Lacolley

и другие.

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101772 - 101772

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Xenogeneic versus allogeneic serum and macromolecular crowding in human tenocyte cultures DOI Creative Commons
Andrea Rampin, Andrea Rossoni,

Lefki Chaniotaki

и другие.

European Journal of Cell Biology, Год журнала: 2024, Номер 103(3), С. 151445 - 151445

Опубликована: Июль 14, 2024

Allogeneic serum and tissue-specific extracellular matrix have been shown to maintain permanently differentiated cell phenotype in culture. This is of particular importance for human tenocytes, a population that readily loses its function during ex vivo With these mind, herein we extracted tenocytes using either foetal bovine or serum, cultured them the absence presence carrageenan Ficoll®, most widely used macromolecular crowding agents (to induce deposition), assessed cellular function, via metabolic activity, viability, proliferation immunofluorescence collagen related molecules, non-collagenous molecules transmembrane molecules. At day 7, longest time point assessed, neither nor Ficoll® significantly affected viability increased activity proliferation. crowding, cells deposited lower type VI, biglycan, versican tenomodulin than serum. Interestingly, at comparison no group, induced highest effect, as judged by number (collagen I, IV, V, transforming growth factor β1, metalloproteinase 14, lumican, versican, scleraxis integrin α2β1). These data, although contradict previous observations where outperformed same time, support use development cell-based medicines.

Язык: Английский

Процитировано

2
Crowd movement monitoring in academic buildings: a reinforcement learning approach DOI

T. Geetha,

C. Subba Rao, C. Chellaswamy

и другие.

Multimedia Tools and Applications, Год журнала: 2024, Номер unknown

Опубликована: Апрель 22, 2024

Язык: Английский

Процитировано

1
Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing Macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 18, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) has a good diastolic and capacity, their altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs always exist in synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense touch detect crowding changes. However, unclear whether macromolecular (MMC) can help VSMCs. Purpose To study obtain phenotypic, behavioral gene expression changes after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay;VSMCs survival activity were Calcein/PI cell toxicity assay CCK-8 assays༛VSMCs migration WB wound healing assay༛Gene transcriptomic analysis RT-qPCR. Results 225 µg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic markers VSMCs, length shortened, proliferation decreased, inhibited. Specifically, 856 genes up-regulated 1207 down-regulated. These alterations primarily affected cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton process while down-regulated mainly Conclusion showed sensing culture environment's crowding, which be maintained concentration CR.

Язык: Английский

Процитировано

0
Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

и другие.

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Апрель 22, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) results in good diastolic and capacities, its altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs exist a synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense detecting changes crowding. However, unclear whether macromolecular crowding (MMC) helps phenotype. Purpose This study aimed explore phenotypic, behavioral gene expression after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay; survival activity were calcein/PI cell toxicity CCK-8 assays; phenotypes migration WB wound healing transcriptomic analysis RT-qPCR. Results Notably, 225 μg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic marker VSMCs, shortened length, decreased proliferation, inhibited migration. VSMCs. Specifically, 856 genes upregulated 1207 downregulated. These alterations primarily cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton processes whereas downregulated mainly Conclusions showed can sensing culture environment, which be maintained CR. Graphical

Язык: Английский

Процитировано

0