Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: April 22, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) results in good diastolic and capacities, its altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs exist a synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense detecting changes crowding. However, unclear whether macromolecular crowding (MMC) helps phenotype. Purpose This study aimed explore phenotypic, behavioral gene expression after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay; survival activity were calcein/PI cell toxicity CCK-8 assays; phenotypes migration WB wound healing transcriptomic analysis RT-qPCR. Results Notably, 225 μg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic marker VSMCs, shortened length, decreased proliferation, inhibited migration. VSMCs. Specifically, 856 genes upregulated 1207 downregulated. These alterations primarily cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton processes whereas downregulated mainly Conclusions showed can sensing culture environment, which be maintained CR. Graphical

Language: Английский

Underestimated role of macromolecular crowding in bioengineered in vitro models of health and diseases DOI Creative Commons
Jérémy Lagrange,

G. Velde,

Patrick Lacolley

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101772 - 101772

Published: April 1, 2025

Language: Английский

Citations

0
Xenogeneic versus allogeneic serum and macromolecular crowding in human tenocyte cultures DOI Creative Commons
Andrea Rampin, Andrea Rossoni,

Lefki Chaniotaki

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 103(3), P. 151445 - 151445

Published: July 14, 2024

Allogeneic serum and tissue-specific extracellular matrix have been shown to maintain permanently differentiated cell phenotype in culture. This is of particular importance for human tenocytes, a population that readily loses its function during ex vivo With these mind, herein we extracted tenocytes using either foetal bovine or serum, cultured them the absence presence carrageenan Ficoll®, most widely used macromolecular crowding agents (to induce deposition), assessed cellular function, via metabolic activity, viability, proliferation immunofluorescence collagen related molecules, non-collagenous molecules transmembrane molecules. At day 7, longest time point assessed, neither nor Ficoll® significantly affected viability increased activity proliferation. crowding, cells deposited lower type VI, biglycan, versican tenomodulin than serum. Interestingly, at comparison no group, induced highest effect, as judged by number (collagen I, IV, V, transforming growth factor β1, metalloproteinase 14, lumican, versican, scleraxis integrin α2β1). These data, although contradict previous observations where outperformed same time, support use development cell-based medicines.

Language: Английский

Citations

2
Crowd movement monitoring in academic buildings: a reinforcement learning approach DOI

T. Geetha,

C. Subba Rao, C. Chellaswamy

et al.

Multimedia Tools and Applications, Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Language: Английский

Citations

1
Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing Macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 18, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) has a good diastolic and capacity, their altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs always exist in synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense touch detect crowding changes. However, unclear whether macromolecular (MMC) can help VSMCs. Purpose To study obtain phenotypic, behavioral gene expression changes after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay;VSMCs survival activity were Calcein/PI cell toxicity assay CCK-8 assays༛VSMCs migration WB wound healing assay༛Gene transcriptomic analysis RT-qPCR. Results 225 µg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic markers VSMCs, length shortened, proliferation decreased, inhibited. Specifically, 856 genes up-regulated 1207 down-regulated. These alterations primarily affected cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton process while down-regulated mainly Conclusion showed sensing culture environment's crowding, which be maintained concentration CR.

Language: Английский

Citations

0
Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing macromolecular crowding in vitro DOI Creative Commons
Qing Liu,

Hongjing Jiang,

Yin‐Di Wu

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: April 22, 2024

Abstract Background The contractile phenotype of vascular smooth muscle cells (VSMCs) results in good diastolic and capacities, its altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs exist a synthetic vitro, making it challenging to maintain research. It widely recognized that common medium vitro significantly less crowded than vivo environment. Additionally, have heightened sense detecting changes crowding. However, unclear whether macromolecular crowding (MMC) helps phenotype. Purpose This study aimed explore phenotypic, behavioral gene expression after increasing degree by adding carrageenan (CR). Methods was examined dynamic light scattering assay; survival activity were calcein/PI cell toxicity CCK-8 assays; phenotypes migration WB wound healing transcriptomic analysis RT-qPCR. Results Notably, 225 μg/mL CR increased did not affect survival. Simultaneously, promoted contraction phenotypic marker VSMCs, shortened length, decreased proliferation, inhibited migration. VSMCs. Specifically, 856 genes upregulated 1207 downregulated. These alterations primarily cellular ion channel transport, microtubule movement, respiratory metabolism, amino acid extracellular matrix synthesis. involved cytoskeleton processes whereas downregulated mainly Conclusions showed can sensing culture environment, which be maintained CR. Graphical

Language: Английский

Citations

0