International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(9), С. 4267 - 4267
Опубликована: Апрель 30, 2025
Designing
a
highly
sensitive
and
efficient
functionalized
electrode
for
precise
drug
analysis
remains
significant
challenge.
In
this
work,
an
electrochemical
sensor
based
on
glassy
carbon
(GCE)
modified
with
phenyl
diazonium
salts
(ph)
electrochemically
reduced
graphene
oxide
(ERGO),
labeled
GCE/ph/ERGO,
was
developed
the
detection
of
paracetamol
(PAR)
in
pharmaceutical
matrices
using
square
wave
voltammetry
(SWV).
The
characterized
by
scanning
electron
microscopy
(SEM),
impedance
spectroscopy
(EIS),
cyclic
(CV).
Compared
to
bare
GCE,
GCE/ph/ERGO
demonstrated
significantly
improved
conductivity
anodic
current
peak
PAR
over
two
orders
magnitude
higher,
indicating
substantial
enhancement
performance.
Under
optimized
conditions,
exhibited
low
limit
18.2
nM
quantification
60.6
nM.
Precision
studies
yielded
relative
standard
deviations
(RSDs)
below
8%.
excellent
selectivity
presence
common
excipients
high
accuracy
generic
formulations,
results
comparable
those
obtained
HPLC
technique.
These
findings
confirm
sensor's
reliability,
stability,
robustness,
suitability
routine
samples.
Nanoscale,
Год журнала:
2024,
Номер
16(26), С. 12586 - 12598
Опубликована: Янв. 1, 2024
In
situ
monitoring
of
H2O2
in
cellular
microenvironments
plays
a
critical
role
the
early
diagnosis
and
pretreatment
cancer,
but
is
limited
by
lack
efficient
low-cost
strategies
for
large-scale
preparation
real-time
biosensors.
Herein,
universal
strategy
MXene-based
composite
inks
combined
with
scalable
screen-printing
process
validated
manufacturing
electrochemical
biosensors
detection
secreted
from
live
cells.
Compositing
biocompatible
carboxymethyl
cellulose
(CMCS)
excellent
conductive
MXene,
water-based
ink
electrode
(MXene/CMCS)
tunable
viscosity
efficiently
printed
desirable
printing
accuracy.
Subsequently,
MXene/CMCS@HRP
biosensor
exhibits
stable
performance
through
HRP
nanoflower
modification,
showing
rapid
electron
transport
high
electrocatalytic
capacity,
demonstrating
low
limit
(0.29
μM)
wide
linear
range
(0.5
μM-3
mM),
superior
sensitivity
(56.45
μA
mM-1
cm-2),
long-term
stability
anti-interference
ability.
Moreover,
this
effectively
employed
HeLa
cells,
revealing
good
biocompatibility
outstanding
biosensing
capability.
This
proposed
not
only
extends
possibility
biomedical
devices,
also
provides
promising
approach
treatment
cancer.
Abstract
Electrochemical
biosensors
fabricated
based
on
nucleic
acids
have
shown
great
potential
for
cancer
recognition
because
of
their
low
cost,
fast
feedback,
high
sensitivity,
and
easy
operation.
This
review
will
demonstrate
the
impression
recent
advances
applications
electrochemical
that
are
acid-based
detection.
We
compare
formulated
with
those
formed
antibodies
highlight
some
examples
developed
detection,
such
as
use
DNA
or
RNA
aptamers
to
detect
prostate-specific
antigens,
microRNA-21,
carcinoembryonic
antigens.
discuss
rewards
drawbacks
these
challenges
they
face,
stability,
reproducibility,
interference,
standardization.
also
suggest
possible
directions
opportunities
future
research
development,
developing
novel
acid
elements,
exploring
new
transducer
materials
configurations,
designing
signal
amplification
strategies,
integrating
microfluidic
devices
portable
instruments,
evaluating
in
clinical
settings
actual
samples
from
patients
healthy
donors.
Overall,
we
believe
offer
an
auspicious
alternative
conventional
methods
detection
contribute
early
diagnosis
effective
treatment.
Graphical
ACS Omega,
Год журнала:
2023,
Номер
8(3), С. 3423 - 3428
Опубликована: Янв. 9, 2023
A
factor
closely
associated
with
renal
disease
status
in
clinical
diagnosis
is
abnormal
human
serum
albumin
(HSA)
concentration
levels
body
fluids
urine,
serum,
etc.
The
surface
stress
biosensor
was
developed
as
a
new
type
of
to
detect
protein
molecule
and
has
wide
range
applications.
However,
further
sensitivity
improvement
required
achieve
higher
detection
performance.
Herein,
MXene/PDMS/Fe3O4/PDMS
the
multilayer
heterogeneous
membrane
(MHBios)
based
on
coupling
magnetic
field,
electric
field
successfully
high
HSA
through
sensitization.
modified
antibody
specifically
binds
at
AuNP
layer,
allowing
convert
caused
by
PDMS
film
deformation
into
an
electrical
signal.
When
exposed
uniform
conductive
path
layer
reshaped
force
amplified
film,
enhancing
conversion
biological
signals
signals.
results
exhibited
that
limit
(LOD)
MHBios
78
ng/mL
when
0-50
μg/mL,
which
markedly
lower
than
minimum
diagnostic
microalbuminuria.
Furthermore,
detected
actual
samples,
confirming
potential
for
early
screening.
International Journal of Electrochemical Science,
Год журнала:
2023,
Номер
18(5), С. 100123 - 100123
Опубликована: Март 24, 2023
In
this
work,
a
method
for
the
visual
and
electrochemical
detection
of
breast
cancer
marker
CA15–3
was
developed
based
on
etching
reaction
gold/silver
core-shell
nanoparticles
(Au@Ag
NPs)
by
mercury
ions
(Hg2+).
Two
kinds
nanocomplex
silicon
dioxide
nanospheres-antibodies-mercury
(SiO2
NSs-Ab-Hg2+)
Au@Ag
NPs-antibodies
NPs-Ab)
were
first
prepared
in
detection.
presence
antigen,
sandwich
structure
immuno-complex
formed
through
specific
recognition
antigen
antibody.
Then,
after
addition
pH
4.6
CPBS,
redox
occurred
between
Hg2+
silver
shell.
With
increase
amount
shell
etched
thinner
thinner,
color
changed
obviously.
Visual
could
be
realized
range
30–300
U/mL.
addition,
we
adopted
DPV
signals
generated
to
realize
CA15–3.
The
concentration
0.1–200
U/mL
with
limit
0.05
A
cost-effective,
ultrasensitive
electrochemical
aptasensor
using
V
2
CT
x
MXene
is
developed
for
real-time,
point-of-care
HER-2
breast
cancer
detection,
offering
high
sensitivity,
selectivity,
and
suitability
resource-limited
settings.
Heliyon,
Год журнала:
2024,
Номер
11(1), С. e41338 - e41338
Опубликована: Дек. 18, 2024
AI-optimized
electrochemical
aptasensors
are
transforming
diagnostic
testing
by
offering
high
sensitivity,
selectivity,
and
rapid
response
times.
Leveraging
data-driven
AI
techniques,
these
sensors
provide
a
non-invasive,
cost-effective
alternative
to
traditional
methods,
with
applications
in
detecting
molecular
biomarkers
for
neurodegenerative
diseases,
cancer,
coronavirus.
The
performance
metrics
outlined
the
comparative
table
illustrate
significant
advancements
enabled
integration.
Sensitivity
increases
from
60
75
%
ordinary
85-95
%,
while
specificity
improves
70-80
90-98
%.
This
enhanced
allows
ultra-low
detection
limits,
such
as
10
fM
carcinoembryonic
antigen
(CEA)
20
mucin-1
(MUC1)
using
Electrochemical
Impedance
Spectroscopy
(EIS),
1
pM
prostate-specific
(PSA)
Differential
Pulse
Voltammetry
(DPV).
Similarly,
Square
Wave
(SWV)
potentiometric
have
detected
alpha-fetoprotein
(AFP)
at
5
epithelial
cell
adhesion
molecule
(EpCAM)
100
fM,
respectively.
integration
also
enhances
reproducibility,
reduces
false
positives
negatives
(from
15-20
5-10
%),
significantly
decreases
times
10-15
s
2-3
s).
These
improve
data
processing
speeds
min
per
sample
2-5
min)
calibration
accuracy
(<2
margin
of
error
compared
expanding
application
scope
multi-target
biomarker
detection.
review
highlights
how
position
powerful
tools
personalized
treatment,
point-of-care
testing,
continuous
health
monitoring.
Despite
higher
cost
($500-$1,500/unit),
their
portability
promise
revolutionize
healthcare,
environmental
monitoring,
food
safety,
ultimately
improving
public
outcomes.
