Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)
Опубликована: Июль 4, 2024
Metastasis is the leading cause of mortality in patients with colorectal cancer (CRC) and angiogenesis a crucial factor tumor invasion metastasis. Long noncoding RNAs (lncRNAs) play regulatory functions various biological processes cells, however, roles lncRNAs CRC-associated remain to be elucidated CRC, as do underlying mechanisms. We used bioinformatics screen differentially expressed from TCGA database. LOC101928222 expression was assessed by qRT-PCR. The impact CRC development both vitro vivo. mechanisms were investigated cellular fractionation, RNA-sequencing, mass spectrometric, RNA pull-down, immunoprecipitation, stability, gene-specific m6A assays. upregulated correlated worse outcome. Moreover, shown promote migration, invasion, CRC. Mechanistically, synergized IGF2BP1 stabilize HMGCS2 mRNA through an m6A-dependent pathway, increased cholesterol synthesis and, ultimately, promotion development. In summary, these findings demonstrate novel, LOC101928222-based mechanism involved regulation metastatic potential LOC101928222-HMGCS2-cholesterol pathway may effective target for diagnosing managing
Язык: Английский