Journal of Experimental & Clinical Cancer Research,
Journal Year:
2024,
Volume and Issue:
43(1)
Published: July 4, 2024
Metastasis
is
the
leading
cause
of
mortality
in
patients
with
colorectal
cancer
(CRC)
and
angiogenesis
a
crucial
factor
tumor
invasion
metastasis.
Long
noncoding
RNAs
(lncRNAs)
play
regulatory
functions
various
biological
processes
cells,
however,
roles
lncRNAs
CRC-associated
remain
to
be
elucidated
CRC,
as
do
underlying
mechanisms.
We
used
bioinformatics
screen
differentially
expressed
from
TCGA
database.
LOC101928222
expression
was
assessed
by
qRT-PCR.
The
impact
CRC
development
both
vitro
vivo.
mechanisms
were
investigated
cellular
fractionation,
RNA-sequencing,
mass
spectrometric,
RNA
pull-down,
immunoprecipitation,
stability,
gene-specific
m6A
assays.
upregulated
correlated
worse
outcome.
Moreover,
shown
promote
migration,
invasion,
CRC.
Mechanistically,
synergized
IGF2BP1
stabilize
HMGCS2
mRNA
through
an
m6A-dependent
pathway,
increased
cholesterol
synthesis
and,
ultimately,
promotion
development.
In
summary,
these
findings
demonstrate
novel,
LOC101928222-based
mechanism
involved
regulation
metastatic
potential
LOC101928222-HMGCS2-cholesterol
pathway
may
effective
target
for
diagnosing
managing
