Molecular Neurobiology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 15, 2025
Язык: Английский
Neuroscience, Год журнала: 2025, Номер 572, С. 1 - 10
Опубликована: Март 5, 2025
Язык: Английский
Процитировано
3
Drug Development Research, Год журнала: 2025, Номер 86(1)
Опубликована: Фев. 1, 2025
Oleuropein (OLP), a compound predominantly found in olive leaves, has been known for its numerous biological activities, including antioxidant, anti-inflammatory, and antimicrobial properties. Despite established therapeutic potential, role treating diarrhea not extensively explored. This study aimed to evaluate the antidiarrheal effects of OLP an vivo model investigate molecular interactions using silico docking studies, pharmacokinetic predictions, toxicity analysis. In study, castor oil was used induce 3-day-old chicks, effect tested at doses 10 20 mg/kg. The standard drug, loperamide (LOP) 3 mg/kg, comparison. results showed that both significantly (p < 0.05) reduced diarrheal secretions increased latency, with mg/kg dose demonstrating most effective results. combination (20 mg/kg) LOP (3 further enhanced effect. revealed exhibited strong binding affinities (BAs) key receptor, μ-opioid receptor associated diarrhea, while higher BA (‒8.9 kcal/mol) compared (‒8.7 kcal/mol). Pharmacokinetic analysis favorable properties studies no acute toxicity, LD50 2000 conclusion, findings suggest possesses significant potential through interaction, positioning it as promising natural agent managing diarrhea. Further are warranted fully elucidate mechanisms action clinical applicability.
Язык: Английский
Процитировано
2
Pharmaceutical Science Advances, Год журнала: 2024, Номер 2, С. 100043 - 100043
Опубликована: Июнь 14, 2024
The diterpenoid phytol is evidently acting against anemia in experimental animals. However, the molecular mechanisms behind this issue are yet to be discovered. This study aimed evaluate phytol's effect on methotrexate-induced folate-deficient animals through vivo and silico studies. For this, a total of thirty adult male Swiss albino mice were randomly divided into six different groups, namely normal control (vehicle), negative (folate deficiency inducer, methotrexate 3 mg/kg), standard (folic acid 1.5 two test groups comprising 25 50 mg/kg, combined group composed highest doses phytol. Except for vehicle, all treated with first days (once/day) induce folate deficiency. Then followed by respective treatment once day days. Hemoglobin (Hb) level was measured from peripheral blood (by tail cutting) 1st (before treatment), 4th (after 7th treatment). On other hand, computational studies performed PyMol, PyRex, Discovery Studio, complementary tools. Findings suggest that significantly (p < 0.05) augmented Hb levels altered reduction dose-dependently. combination also animals; however, its slightly lower than individual (standard test). In study, showed good binding capacity (binding energy: −7.0 kcal/mol) dihydrofolate reductase (DHFR). conclusion, may act altering methotrexate's impacts animals, possibly interacting DHFR. Further validated research necessary develop as an anti-anemia drug near future.
Язык: Английский
Процитировано
14
Natural Product Research, Год журнала: 2024, Номер unknown, С. 1 - 17
Опубликована: Апрель 16, 2024
Gigantol, a bibenzyl compound extracted from various medicinal plants, has shown number of biological activities, making it an attractive candidate for potential medical applications. This systematic review aims to shed light on gigantol's promising role in inflammation treatment and its underlying mechanisms. Gigantol exhibits anti-inflammatory properties pre-clinical pharmacological test systems. It effectively reduced the levels pro-inflammatory markers arachidonic acid metabolites through pathways, such as NF-κB, AKT, PI3K, JNK/cPLA2/12-LOX. The in-silico investigations demonstrated that MMP-13 enzyme served most target gigantol with highest binding affinity (docking score = −8.8 kcal/mol). Encouragingly, absorption, distribution, metabolism, excretion, toxicity (ADMET) analysis confirmed compatibility necessary physiochemical, pharmacokinetic, properties, bolstering drug candidate. well-documented could be agent treating near future.
Язык: Английский
Процитировано
12
Phytotherapy Research, Год журнала: 2024, Номер 38(8), С. 3877 - 3898
Опубликована: Май 20, 2024
Abstract Resveratrol is a widely recognized polyphenolic phytochemical found in various plants and their fruits, such as peanuts, grapes, berry fruits. It renowned for its several health advantages. The well known anticancer properties, substantial amount of clinical evidence has also established promise chemotherapeutic agent. This study focuses on assessing the properties resveratrol gaining insight into underlying molecular mechanisms. evaluates biopharmaceutical, toxicological characteristics, utilization to determine suitability further development reliable Therefore, information about preclinical studies was collected from different electronic databases up‐to‐date (2018–2023). Findings this revealed that potent therapeutic benefits against cancers involving mechanisms, induction oxidative stress, cytotoxicity, inhibition cell migration invasion, autophagy, arresting S phase cycle, apoptotic, anti‐angiogenic, antiproliferative effects by regulating pathways including PI3K/AKT, p38/MAPK/ERK, NGFR‐AMPK‐mTOR, so on. However, compound poor oral bioavailability due reduced absorption; limitation overcome applying nanotechnology (nanoformulation resveratrol). Clinical application showed types cancer with no serious adverse effects. We suggest additional extensive check efficacy, safety, long‐term hazards. could involve larger number samples establish drug treatment cancer.
Язык: Английский
Процитировано
10
Journal of Molecular Neuroscience, Год журнала: 2024, Номер 74(3)
Опубликована: Сен. 4, 2024
Язык: Английский
Процитировано
10
Pharmacological Research - Natural Products, Год журнала: 2024, Номер unknown, С. 100124 - 100124
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
10
CNS Neuroscience & Therapeutics, Год журнала: 2025, Номер 31(2)
Опубликована: Фев. 1, 2025
ABSTRACT Background Numerous studies suggest that morellic acid (MOR), highly available in Garcinia plants, has different physiological activities, including anti‐cancer, anti‐oxidant, and anti‐microbial activity. Aim In this investigation, we aimed to demonstrate the anxiolytic activity, along with mechanism behind activity of MOR, using vivo silico studies. Methods For this, used doses MOR (5 10 mg/kg) administered drug intraperitoneally Swiss albino mice (male female). Diazepam (DZP), a positive allosteric modulator GABA A receptor, was as control at dose 2 mg/kg (i.p), vehicle group. test, various test protocols are assess behavioral patterns mice, swing, hole cross, light–dark testing, open field testing. Results This investigation revealed remarkably reduced locomotor dose‐dependent manner produced calming behaviors like DZP. However, findings showed combination DZP synergistically locomotion compared single therapy. On other hand, from computational study, result demonstrated exhibited highest binding scores (−9.2 kcal/mol) towards receptor α3 subunit −7.6 kcal/mol α2 receptor. Whereas, −6.6 −7.3 docking affinity FLU exerted −6.2 −6.3 subunits, respectively. The ligand interacted by forming hydrogen hydrophobic bonds. Conclusion it is recommended more precise comprehensive preclinical investigations be required exact effects conduct clinical trials determine efficacy safety.
Язык: Английский
Процитировано
1
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Март 7, 2025
Язык: Английский
Процитировано
1
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown
Опубликована: Март 11, 2025
Язык: Английский
Процитировано
1