A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Год журнала: 2021, Номер 10

Опубликована: Окт. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Язык: Английский

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A neural circuit for wind-guided olfactory navigation

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Авг. 8, 2022

Abstract To navigate towards a food source, animals frequently combine odor cues about source identity with wind direction location. Where and how these two are integrated to support navigation is unclear. Here we describe pathway the Drosophila fan-shaped body that encodes attractive promotes upwind navigation. We show neurons throughout this encode odor, but not direction. Using connectomics, identify local called h∆C receive input from previously described pathway. exhibit odor-gated, direction-tuned activity, sparse activation of in reproducible direction, activity required for persistent orientation during odor. Based on connectome data, develop computational model showing can promote goal such as an source. Our results suggest processed by separate pathways within goal-directed

Язык: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Год журнала: 2024, Номер 634(8032), С. 139 - 152

Опубликована: Окт. 2, 2024

Язык: Английский

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Whole-brain annotation and multi-connectome cell typing quantifies circuit stereotypy inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 27, 2023

Abstract The fruit fly Drosophila melanogaster combines surprisingly sophisticated behaviour with a highly tractable nervous system. A large part of the fly’s success as model organism in modern neuroscience stems from concentration collaboratively generated molecular genetic and digital resources. As presented our FlyWire companion paper 1 , this now includes first full brain connectome an adult animal. Here we report systematic hierarchical annotation ∼130,000-neuron including neuronal classes, cell types developmental units (hemilineages). This enables any researcher to navigate huge dataset find systems neurons interest, linked literature through Virtual Fly Brain database 2 . Crucially, resource 4,552 types. 3,094 are rigorous consensus validations previously proposed “hemibrain” 3 In addition, propose 1,458 new types, arising mostly fact that spans whole brain, whereas hemibrain derives subvolume. Comparison showed type counts strong connections were largely stable, but connection weights variable within across animals. Further analysis defined simple heuristics for interpretation: stronger than 10 unitary synapses or providing >1% input target conserved. Some increased variability connectomes: most common mushroom body, required learning memory, is almost twice numerous hemibrain. We evidence functional homeostasis adjustments absolute amount excitatory while maintaining excitation-inhibition ratio. Finally, surprisingly, about one third could not yet be reliably identified connectome. therefore suggest should robust inter-individual variation, namely groups cells quantitatively more similar different other same brain. Joint connectomes demonstrates viability utility definition. Our work defines atlas provides both intellectual framework open source toolchain brain-scale comparative connectomics.

Язык: Английский

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Open-source tools for behavioral video analysis: Setup, methods, and best practices

eLife, Год журнала: 2023, Номер 12

Опубликована: Март 22, 2023

Recently developed methods for video analysis, especially models pose estimation and behavior classification, are transforming behavioral quantification to be more precise, scalable, reproducible in fields such as neuroscience ethology. These tools overcome long-standing limitations of manual scoring frames traditional ‘center mass’ tracking algorithms enable analysis at scale. The expansion open-source acquisition has led new experimental approaches understand behavior. Here, we review currently available discuss how set up these labs recording. We also best practices developing using methods, including community-wide standards critical needs the open sharing datasets code, widespread comparisons better documentation users. encourage broader adoption continued development tools, which have tremendous potential accelerating scientific progress understanding brain

Язык: Английский

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Converting an allocentric goal into an egocentric steering signal

Nature, Год журнала: 2024, Номер 626(8000), С. 808 - 818

Опубликована: Фев. 7, 2024

Neuronal signals that are relevant for spatial navigation have been described in many species

Язык: Английский

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A neural circuit architecture for rapid learning in goal-directed navigation

Neuron, Год журнала: 2024, Номер 112(15), С. 2581 - 2599.e23

Опубликована: Май 24, 2024

Anchoring goals to spatial representations enables flexible navigation but is challenging in novel environments when both must be acquired simultaneously. We propose a framework for how Drosophila uses internal of head direction (HD) build goal upon selective thermal reinforcement. show that flies use stochastically generated fixations and directed saccades express heading preferences an operant visual learning paradigm HD neurons are required modify these based on used symmetric setting expose flies' co-evolve the reliability interacting impacts behavior. Finally, we describe rapid new headings may rest behavioral policy whose parameters form genetically encoded circuit architecture. Such evolutionarily structured architectures, which enable rapidly adaptive behavior driven by representations, relevant across species.

Язык: Английский

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A visual pathway for skylight polarization processing in Drosophila

eLife, Год журнала: 2021, Номер 10

Опубликована: Март 23, 2021

Many insects use patterns of polarized light in the sky to orient and navigate. Here, we functionally characterize neural circuitry fruit fly, Drosophila melanogaster , that conveys signals from eye central complex, a brain region essential for fly’s sense direction. Neurons tuned angle polarization ultraviolet are found throughout anterior visual pathway, connecting optic lobes with complex via tubercle bulb, homologous organization ‘sky compass’ pathways described other insects. We detail how consistent, map-like tunings peripheral system is transformed into reduced representation suited flexible processing brain. This study identifies computational motifs transformation, enabling mechanistic comparisons multisensory integration navigation brains

Язык: Английский

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A projectome of the bumblebee central complex

eLife, Год журнала: 2021, Номер 10

Опубликована: Сен. 15, 2021

Insects have evolved diverse and remarkable strategies for navigating in various ecologies all over the world. Regardless of species, insects share presence a group morphologically conserved neuropils known collectively as central complex (CX). The CX is navigational center, involved sensory integration coordinated motor activity. Despite fact that our understanding behavior comes predominantly from ants bees, most what we know about underlying neural circuitry such work fruit flies. Here, aim to close this gap, by providing first comprehensive map major columnar neurons their projection patterns bee. We find numerous components circuit appear be highly between fly bee, but also highlight several key differences which are likely important functional ramifications.

Язык: Английский

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Flight-induced compass representation in the monarch butterfly heading network

Current Biology, Год журнала: 2021, Номер 32(2), С. 338 - 349.e5

Опубликована: Ноя. 24, 2021

Язык: Английский

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