The tectum/superior colliculus as the vertebrate solution for spatial sensory integration and action

Current Biology, Год журнала: 2021, Номер 31(11), С. R741 - R762

Опубликована: Июнь 1, 2021

The superior colliculus, or tectum in the case of non-mammalian vertebrates, is a part brain that registers events surrounding space, often through vision and hearing, but also electrosensation, infrared detection, other sensory modalities diverse vertebrate lineages. This information used to form maps space positions different salient stimuli relation individual. are arranged layers with visual input uppermost layer, senses deeper positions, spatially aligned motor map deepest layer. Here, we will review organization intrinsic function tectum/superior colliculus processed within tectal circuits. We discuss tectal/superior outputs conveyed directly downstream circuits via thalamus cortical areas control various aspects behavior. evolutionarily conserved among all tailored specialties each lineage, its roles have shifted emergence cerebral cortex mammals. illustrate both divergent properties evolution by comparing processing lampreys belonging oldest group extant larval zebrafish, rodents, vertebrates including primates.

Язык: Английский

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A cell atlas of the chick retina based on single-cell transcriptomics

eLife, Год журнала: 2021, Номер 10

Опубликована: Янв. 4, 2021

Retinal structure and function have been studied in many vertebrate orders, but molecular characterization has largely confined to mammals. We used single-cell RNA sequencing (scRNA-seq) generate a cell atlas of the chick retina. identified 136 types plus 14 positional or developmental intermediates distributed among six classes conserved across vertebrates – photoreceptor, horizontal, bipolar, amacrine, retinal ganglion, glial cells. To assess morphology molecularly defined types, we adapted method for CRISPR-based integration reporters into selectively expressed genes. For Müller glia, found that transcriptionally distinct cells were regionally localized along anterior-posterior, dorsal-ventral, central-peripheral axes. also immature horizontal cell, oligodendrocyte persist late embryonic stages. Finally, analyzed relationships chick, mouse, primate types. Our results provide foundation anatomical, physiological, evolutionary, studies avian visual system.

Язык: Английский

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Evolution of neuronal cell classes and types in the vertebrate retina

Nature, Год журнала: 2023, Номер 624(7991), С. 415 - 424

Опубликована: Дек. 13, 2023

Abstract The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs 1 . Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, reptile, teleost fish lamprey. We found high molecular conservation six retinal classes (photoreceptors, horizontal cells, bipolar amacrine ganglion cells (RGCs) Müller glia), with variation related evolutionary distance. Major subclasses were also conserved, whereas among within or was more pronounced. However, an integrative analysis revealed that numerous are shared species, based on gene expression programmes likely trace back early ancestral vertebrate. degree increased outer (photoreceptors) inner (RGCs), suggesting evolution acts preferentially shape output. Finally, identified rodent orthologues midget RGCs, which comprise than 80% RGCs human retina, subserve high-acuity vision, previously believed be restricted primates 2 By contrast, mouse large receptive fields around 2% RGCs. Projections both primate orthologous overrepresented thalamus, supplies primary cortex. suggest innovations, descendants evolutionarily ancient decreased size number as evolved, thereby facilitating acuity cortical processing information.

Язык: Английский

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Evolution of central neural circuits: state of the art and perspectives

Nature reviews. Neuroscience, Год журнала: 2022, Номер 23(12), С. 725 - 743

Опубликована: Окт. 26, 2022

Язык: Английский

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A single-cell resolution gene expression atlas of the larval zebrafish brain

Science Advances, Год журнала: 2023, Номер 9(8)

Опубликована: Фев. 22, 2023

The advent of multimodal brain atlases promises to accelerate progress in neuroscience by allowing silico queries neuron morphology, connectivity, and gene expression. We used multiplexed fluorescent situ RNA hybridization chain reaction (HCR) technology generate expression maps across the larval zebrafish for a growing set marker genes. data were registered Max Planck Zebrafish Brain (mapzebrain) atlas, thus covisualization expression, single-neuron tracings, expertly curated anatomical segmentations. Using post hoc HCR labeling immediate early cfos, we mapped responses prey stimuli food ingestion freely swimming larvae. This unbiased approach revealed, addition previously described visual motor areas, cluster neurons secondary gustatory nucleus, which express calb2a, as well specific neuropeptide Y receptor, project hypothalamus. discovery exemplifies power this new atlas resource neurobiology.

Язык: Английский

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Ancestral photoreceptor diversity as the basis of visual behaviour

Nature Ecology & Evolution, Год журнала: 2024, Номер 8(3), С. 374 - 386

Опубликована: Янв. 22, 2024

Язык: Английский

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Transcriptomic neuron types vary topographically in function and morphology

Nature, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Abstract Neuronal phenotypic traits such as morphology, connectivity and function are dictated, to a large extent, by specific combination of differentially expressed genes. Clusters neurons in transcriptomic space correspond distinct cell types some cases—for example, Caenorhabditis elegans 1 retinal ganglion cells 2–4 —have been shown share morphology function. The zebrafish optic tectum is composed spatial array that transforms visual inputs into motor outputs. Although the visuotopic map continuous, subregions functionally specialized 5,6 . Here, uncover cell-type architecture tectum, we transcriptionally profiled its neurons, revealing more than 60 organized anatomical layers. We measured responses thousands tectal two-photon calcium imaging matched them with their transcriptional profiles. Furthermore, characterized morphologies identified using transgenic lines. Notably, found similar can diverge shape, responses. Incorporating coordinates within volume revealed morphologically defined subclusters individual clusters. Our findings demonstrate extrinsic, position-dependent factors expand repertoire genetically neurons.

Язык: Английский

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Brain-wide visual habituation networks in wild type and fmr1 zebrafish

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Фев. 16, 2022

Abstract Habituation is a form of learning during which animals stop responding to repetitive stimuli, and deficits in habituation are characteristic several psychiatric disorders. Due technical challenges, the brain-wide networks mediating poorly understood. Here we report calcium imaging larval zebrafish repeated visual looming stimuli. We show that different functional categories loom-sensitive neurons located locations throughout brain, both properties their resulting behavior can be modulated by stimulus saliency timing. Using graph theory, identify circuit habituates minimally, moderately habituating midbrain population proposed mediate sensorimotor transformation, downstream elements responsible for higher order representations delivery behavior. Zebrafish larvae carrying mutation fmr1 gene have systematic shift toward sustained premotor activity this network, slower behavioral habituation.

Язык: Английский

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Myelination-independent functions of oligodendrocyte precursor cells in health and disease

Nature Neuroscience, Год журнала: 2023, Номер 26(10), С. 1663 - 1669

Опубликована: Авг. 31, 2023

Язык: Английский

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Retinal ganglion cell repopulation for vision restoration in optic neuropathy: a roadmap from the RReSTORe Consortium

Molecular Neurodegeneration, Год журнала: 2023, Номер 18(1)

Опубликована: Сен. 21, 2023

Retinal ganglion cell (RGC) death in glaucoma and other optic neuropathies results irreversible vision loss due to the mammalian central nervous system's limited regenerative capacity. RGC repopulation is a promising therapeutic approach reverse from if newly introduced neurons can reestablish functional retinal thalamic circuits. In theory, RGCs might be repopulated through transplantation of stem cell-derived or via induction endogenous transdifferentiation. The Repopulation, Stem Cell Transplantation, Optic Nerve Regeneration (RReSTORe) Consortium was established address challenges associated with repair visual pathway neuropathy. 2022, RReSTORe initiated ongoing international collaborative discussions advance field has identified five critical areas focus: (1) development differentiation, (2) Transplantation methods models, (3) survival, maturation, host interactions, (4) Inner wiring, (5) Eye-to-brain connectivity. Here, we discuss most pertinent questions that exist on path clinical translation suggest experimental directions propel this work going forward. Using these subtopic discussion groups (SDGs) as framework, multidisciplinary approaches restore diseased by leveraging groundbreaking insights developmental neuroscience, biology, molecular optical imaging, animal models neuropathy, immunology & immunotolerance, neuropathology neuroprotection, materials science biomedical engineering, neuroscience. While significant hurdles remain, Consortium's efforts provide comprehensive roadmap for advancing hold potential transformative progress restoring patients suffering neuropathies.

Язык: Английский

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