PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities DOI Creative Commons

R.W. Fink,

Shosei Imai,

Nala Gockel

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Abstract Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio red-shifted posed significant challenges. More critically, several red-fluorescent exhibit photoswitching when exposed to blue light, thereby limiting applicability in all-optical experimental approaches. Here, we present the development PinkyCaMP, first mScarlet-based Ca sensor that outperforms sensors brightness, photostability, ratio, compatibility with optogenetics neurotransmitter imaging. PinkyCaMP is well-tolerated by neurons, showing no toxicity or aggregation, both vitro vivo . All approaches, including single-photon excitation methods such as fiber photometry, widefield imaging, miniscope well two-photon awake mice, fully compatible PinkyCaMP.

Язык: Английский

The thalamic reticular nucleus orchestrates social memory DOI Creative Commons

Feidi Wang,

Huan Sun,

Mingyue Chen

и другие.

Neuron, Год журнала: 2024, Номер 112(14), С. 2368 - 2385.e11

Опубликована: Май 2, 2024

Social memory has been developed in humans and other animals to recognize familiar conspecifics is essential for their survival reproduction. Here, we demonstrated that parvalbumin-positive neurons the sensory thalamic reticular nucleus (sTRN

Язык: Английский

Процитировано

10
Serotonin and neurotensin inputs in the vCA1 dictate opposing social valence DOI Creative Commons

Julia M Zorab,

Huanhuan Li, Richa Awasthi

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Апрель 30, 2025

The ability to evaluate valence of a social agent based on experience is essential for an animal's survival in its group1. Although hippocampal circuits have been implicated distinguishing novel and familiar conspecifics2-7, it remains unclear how constructed the basis history what mechanisms underlie heightened versatility dynamic relationships. Here we demonstrate that ventral (v)CA1 integrates serotonin (5-HT) inputs from dorsal raphe neurotensin paraventricular nucleus thalamus (PVT) determine positive or negative conspecific representations. Specifically, during appetitive interaction 5-HT released into vCA1 disinhibits pyramidal neurons through 5-HT1B receptors, whereas aversive potentiates directly NTR1s. Optogenetic silencing PVT impairs valence, respectively, excitation flexibly switches assignment. These results show rewarding experiences are linked identity converging signals instruct opposing represent synaptic switch flexible computation.

Язык: Английский

Процитировано

1
Visuotactile integration facilitates mirror-induced self-directed behavior through activation of hippocampal neuronal ensembles in mice DOI Creative Commons
Jun Yokose, William D. Marks, Takashi Kitamura

и другие.

Neuron, Год журнала: 2023, Номер 112(2), С. 306 - 318.e8

Опубликована: Дек. 5, 2023

Язык: Английский

Процитировано

13
Associative learning and recollection of olfactory memory during the respiratory cycle in mammals: how is the self cognized in consciousness? DOI Creative Commons
Kensaku Mori, Hitoshi Sakano

Frontiers in Neuroscience, Год журнала: 2025, Номер 18

Опубликована: Янв. 17, 2025

When we are awake and relaxed, various memory-scenes come up in our mind by spontaneous activation of memory engrams. We find ourselves the memory-scene longing for it present self. The scene is also recollected sensory inputs from surrounding world learned behavioral decisions. It well experienced that odorants act as strong cues remembering associated memory. Associative learning odor signals object cognition enables us to predict cognitive imagery an environmental object. Here, discuss neural network connecting olfactory cortices higher areas dynamically switches processing mode feedforward top-down. These processes correlated with respiratory cycle form recollect odor-object associative infer during inhalation phase, drive burst firings a specific subset pyramidal cells cortex. In contrast, subsequent late-exhalation top-down scene-signals activate again same those activated signals. Reactivation exhalation phase may induce plastic changes inter-areal synaptic connections associative-learning this perspective article, will self mammalian system.

Язык: Английский

Процитировано

0
Intranasal Transplantation of Microbiota Derived from Parkinson’s Disease Mice Induced Astrocyte Activation and Neurodegenerative Pathology from Nose to Brain DOI Creative Commons

Yi-Meng Xia,

Mei-Xuan Zhang,

Xiaoyu Ma

и другие.

Brain Sciences, Год журнала: 2025, Номер 15(5), С. 433 - 433

Опубликована: Апрель 23, 2025

Parkinson's disease (PD) is characterized by early-onset olfactory dysfunction preceding motor symptoms, yet its mechanisms remain elusive. Based on the studies microbiota-gut-brain axis, microbiota-nose-brain axis might be involved in pathogenesis of PD. However relative are rare. By consecutive 14-days intranasally transplanting bacteria, we established mice models exhibiting nasal microbiota dysbiosis (NMD), including animal group received intranasal drops fecal bacterial suspension from normal (NB group) and PD (PB group), with animals that only anesthesia used as control group. Then analyzed composition via 16S rRNA sequencing, evaluated functions through behavioral experiments, buried food test, open field pole descent traction test. The neuropathology olfactory-related PD-related brain regions, bulb, pyriform cortex, hippocampus, substantia nigra striatum, was also detected western blotting, immunofluorescence immunohistochemical experiments using antibodies NeuN, TH GFAP. sequencing revealed PB were primarily an increase bacteria associated inflammation Behavioral assessments NMD demonstrated impairments test indicative dysfunction. detecting NeuN GFAP expression, identified neuronal loss astrocytes activation regions adjacent structures, striatum both groups, which may contribute to observed functional disorders. Notably, exposed PD-derived exhibited more pronounced changes severe neuropathology. We present evidence supporting NMD-induced astrocyte neurodegenerative pathology along pathway serve a link between nose brain.

Язык: Английский

Процитировано

0
An inhibitory circuit-based enhancer of DYRK1A function reverses Dyrk1a-associated impairment in social recognition DOI Creative Commons

Yu‐Tzu Shih,

Jason Bondoc Alipio, Amar Sahay

и другие.

Neuron, Год журнала: 2023, Номер 111(19), С. 3084 - 3101.e5

Опубликована: Окт. 1, 2023

Heterozygous mutations in the dual-specificity tyrosine phosphorylation-regulated kinase 1a (Dyrk1a) gene define a syndromic form of autism spectrum disorder. The synaptic and circuit mechanisms mediating DYRK1A functions social cognition are unclear. Here, we identify experience-sensitive mechanism hippocampal mossy fiber-parvalbumin interneuron (PV IN) synapses by which recruits feedforward inhibition CA3 CA2 to promote recognition. We employ genetic epistasis logic cytoskeletal protein, ABLIM3, as substrate DYRK1A. demonstrate that Ablim3 downregulation dentate granule cells adult heterozygous Dyrk1a mice is sufficient restore PV IN-mediated Acute chemogenetic activation INs CA3/CA2 also rescued Together, these findings illustrate how targeting substrates "enhancers function" harbors potential reverse haploinsufficiency-associated impairments.

Язык: Английский

Процитировано

8
The hippocampal CA2 region discriminates social threat from social safety DOI
Pegah Kassraian, Shivani Bigler,

Diana M. Gilly Suarez

и другие.

Nature Neuroscience, Год журнала: 2024, Номер 27(11), С. 2193 - 2206

Опубликована: Окт. 15, 2024

Язык: Английский

Процитировано

2
Comparative analysis of gonadal hormone receptor expression in the postnatal house mouse, meadow vole, and prairie vole brain DOI
Katherine Denney, Melody V. Wu, Simón D. Sun

и другие.

Hormones and Behavior, Год журнала: 2023, Номер 158, С. 105463 - 105463

Опубликована: Ноя. 22, 2023

Язык: Английский

Процитировано

5
Modulation of aggression by social novelty recognition memory in the hippocampal CA2 region DOI Creative Commons
A. Villegas, Steven A. Siegelbaum

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 4, 2024

Abstract The dorsal CA2 subregion (dCA2) of the hippocampus exerts a critical role in social novelty recognition (SNR) memory and promotion aggression. Whether aggression SNR functions dCA2 are related or represent independent processes is unknown. Here we investigated hypotheses that an animal more likely to attack novel compared familiar promotes through its ability discriminate between conspecifics. To test these ideas, conducted multi-day resident intruder (R-I) towards We found mice were familiarized silencing caused profound inhibition than intruder. explore whether how pyramidal neurons encode aggression, recorded their activity using microendoscopic calcium imaging throughout days R-I test. fraction selectively activated inhibited during exploration, dominance, behaviors signals enhanced interaction with conspecific. Based on population activity, set binary linear classifiers accurately decoded was engaged each forms behavior. Of particular interest, accuracy decoding greater intruders, significant cross-day same day but not for familiar-novel pair. Together, findings demonstrate integrates information about behavioral state promote

Язык: Английский

Процитировано

1
Adolescent cannabinoid exposure rescues phencyclidine-induced social deficits through modulation of CA2 transmission DOI
Marta Barrera,

Carla Ramon-Duaso,

Jose Antonio González-Parra

и другие.

Progress in Neurobiology, Год журнала: 2024, Номер 240, С. 102652 - 102652

Опубликована: Июнь 30, 2024

Язык: Английский

Процитировано

1