Toward Optogenetic Hearing Restoration
Annual Review of Neuroscience,
Год журнала:
2024,
Номер
47(1), С. 103 - 121
Опубликована: Апрель 10, 2024
The
cochlear
implant
(CI)
is
considered
the
most
successful
neuroprosthesis
as
it
enables
speech
comprehension
in
majority
of
million
otherwise
deaf
patients.
In
hearing
by
electrical
stimulation
auditory
nerve,
broad
spread
current
from
each
electrode
acts
a
bottleneck
that
limits
transfer
sound
frequency
information.
Hence,
there
remains
major
unmet
medical
need
for
improving
quality
with
CIs.
Recently,
optogenetic
cochlea
has
been
suggested
an
alternative
approach
restoration.
Cochlear
optogenetics
promises
to
more
information,
hence
hearing,
light
can
conveniently
be
confined
space
activate
nerve
within
smaller
tonotopic
ranges.
this
review,
we
discuss
latest
experimental
and
technological
developments
restoration
outline
remaining
challenges
en
route
clinical
translation.
Язык: Английский
Mathematical modeling of cochlear mechanics and disorder
Alexandria Engineering Journal,
Год журнала:
2025,
Номер
123, С. 448 - 459
Опубликована: Март 28, 2025
Язык: Английский
Genetically‐targeted control of the spinal cord and peripheral nervous system
Journal of intelligent medicine.,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
Abstract
Genetically‐targeted
neuromodulation,
such
as
opto‐
or
chemogenetics,
is
important
for
cell‐type
specific
neuromodulation
different
applications.
Modulating
peripheral
nerves
a
daunting
task
due
to
the
wide
variety
of
constraints
in
periphery,
size,
location,
and
relative
distance
other
nerves.
This
has
resulted
many
researchers
developing
innovative
solutions
modulate
nerves,
including
types
light‐emitting
devices
optogenetics,
ligands
designer
drugs
(DREADDs)
use
nanotechnology.
Although
spinal
cord
part
central
nervous
system,
it
often
site
stimulation
modulating
function
periphery
anatomy
same
obstacles
modulation
review
summarizes
current
efforts
genetically‐targeted
grouped
by
applications,
going
through
major
areas
which
advancements
have
been
made.
We
focus
on
vivo
research
rodents
but
also
briefly
discuss
work
nonhuman
primates
(NHPs).
Some
clinical
translation,
long‐term
adeno‐associated
virus
(AAV)
safety,
are
discussed
some
noninvasive,
nonspecific
technologies
mentioned.
Язык: Английский
Projection-specific and reversible functional blockage in the association cortex of macaque monkeys
Seiichirou Yokoo,
Takayasu Higo,
Florian Gérard-Mercier
и другие.
Neuroscience Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
The
functional
manipulation
techniques
based
on
optogenetics
have
been
widely
and
effectively
utilized
in
the
rodent
brain.
However,
applications
of
these
to
macaque
cerebral
cortex,
particularly
those
prefrontal
limited
due
extensive
size
complex
organization
each
area.
In
this
study,
we
developed
projection-specific
reversible
blockade
methods
applicable
areas
chemogenetic
techniques.
chemogenetics,
once
a
pair
viral
vectors
has
injected
into
regions
projection
source
destination,
blockage
can
be
initiated
through
oral,
intravenous,
or
intramuscular
administration
an
appropriate
pharmaceutical
agent.
Two
were
using
two
different
effector
proteins,
inhibitory
DREADD,
hM4Di,
tetanus
toxin,
given
substantial
discrepancy
on-off
time
course
between
two.
Cre-DIO
system
was
combined
with
Tet-On
toxin.
effectiveness
evaluated
by
developing
electrophysiological
assay
photic
stimulation
field
potential
recordings.
Язык: Английский
Multispecies initial numerical validation of an efficient algorithm prototype for auditory brainstem response hearing threshold estimation
The Journal of the Acoustical Society of America,
Год журнала:
2024,
Номер
156(3), С. 1674 - 1687
Опубликована: Сен. 1, 2024
The
auditory
brainstem
response
(ABR)
can
be
used
to
evaluate
hearing
sensitivity
of
animals.
However,
typical
measurement
protocols
are
time-consuming.
Here,
an
adaptive
algorithm
is
proposed
for
efficient
ABR
threshold
estimation.
relies
on
the
update
predicted
from
a
Gaussian
process
model
as
data
collected
using
iteratively
optimized
stimuli.
To
validate
algorithm,
estimation
simulated
by
adaptively
subsampling
pre-collected
datasets.
experiment
performed
5
datasets
mouse,
budgerigar,
gerbil,
and
guinea
pig
ABRs
(27
ears).
contain
68–106
stimuli
conditions,
configured
terminate
after
20
conditions.
estimate
compared
against
human
rater
estimates
who
visually
inspected
full
waveform
stacks.
matches
within
10
dB,
averaged
over
frequency,
15
27
ears
while
reducing
number
conditions
factor
3–5
standard
practice.
intraclass
correlation
coefficient
0.81
with
95%
upper
lower
bounds
at
0.74
0.86,
indicating
moderate
good
reliability
between
estimates.
results
demonstrate
feasibility
Bayesian
procedure
rapid
Язык: Английский
A bibliometric profile of optogenetics: quantitative and qualitative analyses
Frontiers in Neuroscience,
Год журнала:
2023,
Номер
17
Опубликована: Июнь 22, 2023
Optogenetics
is
a
rapidly
developing
field
combining
optics
and
genetics,
with
promising
applications
in
neuroscience
beyond.
However,
there
currently
lack
of
bibliometric
analyses
examining
publications
this
area.Publications
on
optogenetics
were
gathered
from
the
Web
Science
Core
Collection
Database.
A
quantitative
analysis
was
conducted
to
gain
insights
into
annual
scientific
output,
distribution
authors,
journals,
subject
categories,
countries,
institutions.
Additionally,
qualitative
analysis,
such
as
co-occurrence
network
thematic
theme
evolution,
performed
identify
main
areas
trends
articles.A
total
6,824
included
for
analysis.
The
number
articles
has
grown
since
2010,
an
growth
rate
52.82%.
Deisseroth
K,
Boyden
ES,
Hegemann
P
most
prolific
contributors
field.
United
States
contributed
(3,051
articles),
followed
by
China
(623
articles).
majority
optogenetics-related
are
published
high-quality
including
NATURE,
SCIENCE,
CELL.
These
mainly
belong
four
subjects:
neurosciences,
biochemistry
molecular
biology,
neuroimaging,
materials
science.
Co-occurrence
keyword
identified
three
clusters:
optogenetic
components
techniques,
neural
circuitry,
disease.The
results
suggest
that
research
flourishing,
focusing
techniques
their
circuitry
exploration
disease
intervention.
expected
remain
hot
topic
various
fields
future.
Язык: Английский
Devising a framework of optogenetic coding in the auditory pathway: Insights from auditory midbrain recordings
Brain stimulation,
Год журнала:
2023,
Номер
16(5), С. 1486 - 1500
Опубликована: Сен. 1, 2023
Cochlear
implants
(CIs)
restore
activity
in
the
deafened
auditory
system
via
electrical
stimulation
of
nerve.
As
spread
electric
current
biological
tissues
is
rather
broad,
spectral
information
provided
by
CIs
limited.
Optogenetic
nerve
has
been
suggested
for
artificial
sound
coding
with
improved
selectivity,
as
light
can
be
conveniently
confined
space.
Yet,
foundations
optogenetic
strategies
remain
to
established.
Here,
we
parametrized
stimulus-response-relationships
pathway
gerbils
stimulation.
Upon
activation
waveguide-based
spiral
ganglion,
recorded
neuronal
midbrain,
which
neural
representations
spectral,
temporal,
and
intensity
found.
Screening
a
wide
range
optical
stimuli
taking
properties
CI
emitters
into
account,
aimed
optimize
stimulus
paradigms
potent
energy-efficient
pathway.
We
report
that
efficient
builds
on
integration
millisecond
built
from
microsecond
pulses,
optimally
accommodate
power-efficient
laser
diode
operation.
Moreover,
performed
an
activity-level-dependent
comparison
acoustic
order
estimate
dynamic
maximal
amenable
single
channel
encoding,
indicate
it
complies
well
speech
comprehension
typical
conversation
(65
dB).
Our
results
provide
first
framework
development
future
hearing
restoration.
Язык: Английский
Multi-species numerical validation of an efficient algorithm for auditory brainstem response hearing threshold estimation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 20, 2024
Abstract
The
Auditory
Brainstem
Response
(ABR)
can
be
used
to
evaluate
hearing
sensitivity
of
animals
who
are
unable
respond
behavioral
tasks.
However,
typical
data
collection
methods
time
consuming;
decreasing
the
measurement
may
save
resources
or
allow
researchers
spend
more
on
other
Here,
an
adaptive
algorithm
is
proposed
for
efficient
estimation
ABR
thresholds.
relies
online
update
predicted
threshold
from
a
Gaussian
process
model
as
collected
using
iteratively
optimized
stimuli.
To
validate
algorithm,
simulated
by
adaptively
sub-sampling
pre-collected
datasets
which
stimuli
were
systematically
varied
in
frequency
and
level.
experiment
performed
5
Mouse
(2
different
datasets),
Budgerigar,
Gerbil,
Guinea
Pig
ABRs
laboratories,
with
total
27
ears.
original
contain
between
68
106
conditions,
while
run
up
20
conditions.
estimate
compared
against
human
rater
estimates
view
full
dataset.
matches
within
10
dB,
averaged
over
frequency,
19
out
procedure
able
provide
that
comparable
estimated
thresholds
reducing
factor
3
5.
standard
deviation
successive
runs
smaller
than
inter-human
differences,
indicating
adequate
test/retest
reliability.
Язык: Английский
Improved optogenetic modification of the spiral ganglion neurons for future optical cochlear implants
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 12, 2024
Abstract
Optogenetic
stimulation
has
become
a
promising
approach
for
restoring
lost
body
function.
For
example,
partial
restoration
of
vision
been
achieved
in
blind
patient
and
proof-of-concept
demonstrated
optogenetic
hearing
rodents.
In
order
to
prepare
clinical
translation
restoration,
efficient
safe
modification
spiral
ganglion
neurons
(SGNs)
the
mature
cochlea
remains
be
developed.
Here,
we
established
microcatheter-based
administration
adeno-associated
virus
(AAV)
scala
tympani
Mongolian
gerbils
compared
it
previously
developed
AAV-injection
into
ganglion.
We
probed
potential
AAV-PHP.S
capsid
express
channelrhodopsins
(ChRs)
under
control
human
synapsin
promotor
SGNs
deafened
gerbils.
Using
microcatheter
approach,
but
not
with
AAV-modiolus
injection,
reliable
ChR
expression
SGN
enabling
auditory
pathway
80%
treated
animals.
Yet,
efficiency
transduction
was
modest
only
∼30%
ChR-expressing
SGNs.
Moreover,
encountered
off-target
hair
cells
both
approaches,
central
nervous
system
using
administration.
Comparing
brainstem
responses
without
cell
confirmed
that
were
primary
site
pathway.
Язык: Английский