Multi-species numerical validation of an efficient algorithm for auditory brainstem response hearing threshold estimation DOI Creative Commons
Erik Alan Petersen, Yi Shen

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 20, 2024

Abstract The Auditory Brainstem Response (ABR) can be used to evaluate hearing sensitivity of animals who are unable respond behavioral tasks. However, typical data collection methods time consuming; decreasing the measurement may save resources or allow researchers spend more on other Here, an adaptive algorithm is proposed for efficient estimation ABR thresholds. relies online update predicted threshold from a Gaussian process model as collected using iteratively optimized stimuli. To validate algorithm, simulated by adaptively sub-sampling pre-collected datasets which stimuli were systematically varied in frequency and level. experiment performed 5 Mouse (2 different datasets), Budgerigar, Gerbil, Guinea Pig ABRs laboratories, with total 27 ears. original contain between 68 106 conditions, while run up 20 conditions. estimate compared against human rater estimates view full dataset. matches within 10 dB, averaged over frequency, 19 out procedure able provide that comparable estimated thresholds reducing factor 3 5. standard deviation successive runs smaller than inter-human differences, indicating adequate test/retest reliability.

Language: Английский

Toward Optogenetic Hearing Restoration DOI
Antoine Huet, Thomas Mager, Christian Goßler

et al.

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 103 - 121

Published: April 10, 2024

The cochlear implant (CI) is considered the most successful neuroprosthesis as it enables speech comprehension in majority of million otherwise deaf patients. In hearing by electrical stimulation auditory nerve, broad spread current from each electrode acts a bottleneck that limits transfer sound frequency information. Hence, there remains major unmet medical need for improving quality with CIs. Recently, optogenetic cochlea has been suggested an alternative approach restoration. Cochlear optogenetics promises to more information, hence hearing, light can conveniently be confined space activate nerve within smaller tonotopic ranges. this review, we discuss latest experimental and technological developments restoration outline remaining challenges en route clinical translation.

Language: Английский

Citations

6
Genetically‐targeted control of the spinal cord and peripheral nervous system DOI Creative Commons
Anakaren Romero-Lozano,

Victoria Koptelova,

Zoya Ahmad

et al.

Journal of intelligent medicine., Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Genetically‐targeted neuromodulation, such as opto‐ or chemogenetics, is important for cell‐type specific neuromodulation different applications. Modulating peripheral nerves a daunting task due to the wide variety of constraints in periphery, size, location, and relative distance other nerves. This has resulted many researchers developing innovative solutions modulate nerves, including types light‐emitting devices optogenetics, ligands designer drugs (DREADDs) use nanotechnology. Although spinal cord part central nervous system, it often site stimulation modulating function periphery anatomy same obstacles modulation review summarizes current efforts genetically‐targeted grouped by applications, going through major areas which advancements have been made. We focus on vivo research rodents but also briefly discuss work nonhuman primates (NHPs). Some clinical translation, long‐term adeno‐associated virus (AAV) safety, are discussed some noninvasive, nonspecific technologies mentioned.

Language: Английский

Citations

0
Mathematical modeling of cochlear mechanics and disorder DOI
Nawal H. Siddig, Abdulla Al‐Ali

Alexandria Engineering Journal, Journal Year: 2025, Volume and Issue: 123, P. 448 - 459

Published: March 28, 2025

Language: Английский

Citations

0
Multispecies initial numerical validation of an efficient algorithm prototype for auditory brainstem response hearing threshold estimation DOI
Erik Alan Petersen, Yi Shen

The Journal of the Acoustical Society of America, Journal Year: 2024, Volume and Issue: 156(3), P. 1674 - 1687

Published: Sept. 1, 2024

The auditory brainstem response (ABR) can be used to evaluate hearing sensitivity of animals. However, typical measurement protocols are time-consuming. Here, an adaptive algorithm is proposed for efficient ABR threshold estimation. relies on the update predicted from a Gaussian process model as data collected using iteratively optimized stimuli. To validate algorithm, estimation simulated by adaptively subsampling pre-collected datasets. experiment performed 5 datasets mouse, budgerigar, gerbil, and guinea pig ABRs (27 ears). contain 68–106 stimuli conditions, configured terminate after 20 conditions. estimate compared against human rater estimates who visually inspected full waveform stacks. matches within 10 dB, averaged over frequency, 15 27 ears while reducing number conditions factor 3–5 standard practice. intraclass correlation coefficient 0.81 with 95% upper lower bounds at 0.74 0.86, indicating moderate good reliability between estimates. results demonstrate feasibility Bayesian procedure rapid

Language: Английский

Citations

1
A bibliometric profile of optogenetics: quantitative and qualitative analyses DOI Creative Commons
Zhonghan Zhou, Xuesheng Wang, Xunhua Li

et al.

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: June 22, 2023

Optogenetics is a rapidly developing field combining optics and genetics, with promising applications in neuroscience beyond. However, there currently lack of bibliometric analyses examining publications this area.Publications on optogenetics were gathered from the Web Science Core Collection Database. A quantitative analysis was conducted to gain insights into annual scientific output, distribution authors, journals, subject categories, countries, institutions. Additionally, qualitative analysis, such as co-occurrence network thematic theme evolution, performed identify main areas trends articles.A total 6,824 included for analysis. The number articles has grown since 2010, an growth rate 52.82%. Deisseroth K, Boyden ES, Hegemann P most prolific contributors field. United States contributed (3,051 articles), followed by China (623 articles). majority optogenetics-related are published high-quality including NATURE, SCIENCE, CELL. These mainly belong four subjects: neurosciences, biochemistry molecular biology, neuroimaging, materials science. Co-occurrence keyword identified three clusters: optogenetic components techniques, neural circuitry, disease.The results suggest that research flourishing, focusing techniques their circuitry exploration disease intervention. expected remain hot topic various fields future.

Language: Английский

Citations

2
Devising a framework of optogenetic coding in the auditory pathway: Insights from auditory midbrain recordings DOI Creative Commons
M Halassa Michael, Bettina Wolf,

Astrid Klinge‐Strahl

et al.

Brain stimulation, Journal Year: 2023, Volume and Issue: 16(5), P. 1486 - 1500

Published: Sept. 1, 2023

Cochlear implants (CIs) restore activity in the deafened auditory system via electrical stimulation of nerve. As spread electric current biological tissues is rather broad, spectral information provided by CIs limited. Optogenetic nerve has been suggested for artificial sound coding with improved selectivity, as light can be conveniently confined space. Yet, foundations optogenetic strategies remain to established. Here, we parametrized stimulus-response-relationships pathway gerbils stimulation. Upon activation waveguide-based spiral ganglion, recorded neuronal midbrain, which neural representations spectral, temporal, and intensity found. Screening a wide range optical stimuli taking properties CI emitters into account, aimed optimize stimulus paradigms potent energy-efficient pathway. We report that efficient builds on integration millisecond built from microsecond pulses, optimally accommodate power-efficient laser diode operation. Moreover, performed an activity-level-dependent comparison acoustic order estimate dynamic maximal amenable single channel encoding, indicate it complies well speech comprehension typical conversation (65 dB). Our results provide first framework development future hearing restoration.

Language: Английский

Citations

2
Improved optogenetic modification of the spiral ganglion neurons for future optical cochlear implants DOI Creative Commons
Anupriya Thirumalai, Jörg Henseler,

Marzieh Enayati

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract Optogenetic stimulation has become a promising approach for restoring lost body function. For example, partial restoration of vision been achieved in blind patient and proof-of-concept demonstrated optogenetic hearing rodents. In order to prepare clinical translation restoration, efficient safe modification spiral ganglion neurons (SGNs) the mature cochlea remains be developed. Here, we established microcatheter-based administration adeno-associated virus (AAV) scala tympani Mongolian gerbils compared it previously developed AAV-injection into ganglion. We probed potential AAV-PHP.S capsid express channelrhodopsins (ChRs) under control human synapsin promotor SGNs deafened gerbils. Using microcatheter approach, but not with AAV-modiolus injection, reliable ChR expression SGN enabling auditory pathway 80% treated animals. Yet, efficiency transduction was modest only ∼30% ChR-expressing SGNs. Moreover, encountered off-target hair cells both approaches, central nervous system using administration. Comparing brainstem responses without cell confirmed that were primary site pathway.

Language: Английский

Citations

0
Multi-species numerical validation of an efficient algorithm for auditory brainstem response hearing threshold estimation DOI Creative Commons
Erik Alan Petersen, Yi Shen

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 20, 2024

Abstract The Auditory Brainstem Response (ABR) can be used to evaluate hearing sensitivity of animals who are unable respond behavioral tasks. However, typical data collection methods time consuming; decreasing the measurement may save resources or allow researchers spend more on other Here, an adaptive algorithm is proposed for efficient estimation ABR thresholds. relies online update predicted threshold from a Gaussian process model as collected using iteratively optimized stimuli. To validate algorithm, simulated by adaptively sub-sampling pre-collected datasets which stimuli were systematically varied in frequency and level. experiment performed 5 Mouse (2 different datasets), Budgerigar, Gerbil, Guinea Pig ABRs laboratories, with total 27 ears. original contain between 68 106 conditions, while run up 20 conditions. estimate compared against human rater estimates view full dataset. matches within 10 dB, averaged over frequency, 19 out procedure able provide that comparable estimated thresholds reducing factor 3 5. standard deviation successive runs smaller than inter-human differences, indicating adequate test/retest reliability.

Language: Английский

Citations

0