Transgene‐free genome editing in poplar

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

Summary Precise gene‐editing methods are valuable tools to enhance genetic traits. Gene editing is commonly achieved via stable integration of a cassette in the plant's genome. However, this technique unfavorable for field applications, especially vegetatively propagated plants, such as many commercial tree species, where cannot be segregated away without breaking constitution elite variety. Here, we describe an efficient method generating gene‐edited Populus tremula × P. alba (poplar) trees incorporating foreign DNA into its Using Agrobacterium tumefaciens , expressed base‐editing construct targeting CCoAOMT1 along with ALS genes positive selection on chlorsulfuron‐containing medium. About 50% regenerated shoots were derived from transient transformation and free T‐DNA. Overall, 7% chlorsulfuron‐resistant T‐DNA free, edited gene nonchimeric. Long‐read whole‐genome sequencing confirmed absence any tested lines. Additionally, evaluated CodA negative marker eliminate lines that stably incorporated their Although latter not essential selecting transgene‐free, shoots, it may prove other genotypes or varieties.

Язык: Английский

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Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

Frontiers in Genome Editing, Год журнала: 2023, Номер 5

Опубликована: Июль 20, 2023

Increased understanding of plant genetics and the development powerful easier-to-use gene editing tools over past century have revolutionized humankind's ability to deliver precise genotypes in crops. Plant transformation techniques are well developed for making transgenic varieties certain crops model organisms, yet reagent delivery regeneration remain key bottlenecks applying technology most Typical protocols produce transgenic, genetically modified (GM) rely on transgenes, chemical selection, tissue culture. make edited (GE) also use even though these may be undesirable final crop product. In some crops, transgenes routinely segregated away during meiosis by performing crosses, thus only a minor concern. other particularly those propagated vegetatively, complex hybrids, or with long generation times, such crosses impractical impossible. This review highlights diverse strategies CRISPR/Cas reagents regenerable cells recover plants without unwanted integration transgenes. Some examples include delivering DNA-free as ribonucleoproteins mRNA, relying expression from non-integrated DNA, using novel mechanisms viruses nanoparticles, unconventional selection methods avoid and/or avoiding culture altogether. These advancing rapidly already enabling scientists precision CRISPR tools.

Язык: Английский

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Woody plant cell walls: Fundamentals and utilization

Molecular Plant, Год журнала: 2023, Номер 17(1), С. 112 - 140

Опубликована: Дек. 15, 2023

Cell walls in plants, particularly forest trees, are the major carbon sink of terrestrial ecosystem. Chemical and biosynthetic features plant cell were revealed early on, focusing mostly on herbaceous model species. Recent developments genomics, transcriptomics, epigenomics, transgenesis, associated analytical techniques enabling novel insights into formation woody walls. Here, we review multilevel regulation wall biosynthesis tree We highlight current approaches to engineering as potential feedstock for materials energy survey reported field tests such engineered transgenic trees. outline opportunities challenges future research better understand type biogenesis more efficient wood modification utilization biomaterials or enhanced capture storage.

Язык: Английский

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Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Forests, Год журнала: 2024, Номер 15(3), С. 441 - 441

Опубликована: Фев. 26, 2024

Forests represent a vital natural resource and play crucial role in climate regulation maintaining biodiversity. However, the growth development of forest trees are increasingly challenged by rising environmental pressures, particularly detrimental abiotic stressors. To address these challenges, genetic transformation technologies have emerged as effective solutions. Despite various difficulties for trees, including prolonged life cycles, diversity, interspecies variations, complex regeneration systems, significant research progress has been achieved tree gene editing, transgenic technology, methods delivering exogenous molecules. These potential to enhance quality, increase productivity, improve resistance stress. This review provides an overview main receptors transformation. Additionally, we summarize several novel techniques, such nanoparticle-mediated transformation, advanced editing delivery carriers, non-genetically modified protein function interference through peptide aptamer. Notably, also place emphasis on referable genes from common crops, together with their improving stress responses. Through this research, aspire achieve sustainable utilization conservation resources, thereby providing substantial support future livelihoods economic development.

Язык: Английский

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CRISPR/Cas-mediated genome editing for efficient tomato breeding: past achievements and future directions

South African Journal of Botany, Год журнала: 2024, Номер 172, С. 277 - 288

Опубликована: Июль 26, 2024

Язык: Английский

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Paving the way towards future‐proofing our crops

Food and Energy Security, Год журнала: 2023, Номер 12(3)

Опубликована: Фев. 3, 2023

Abstract To meet the increasing global demand for food, feed, fibre and other plant‐derived products, a steep increase in crop productivity is scientifically technically challenging imperative. The CropBooster‐P project, response to H2020 call ‘Future proofing our plants’, developing roadmap plant research improve crops critical future of European agriculture by yield, nutritional quality, value non‐food applications sustainability. However, if we want efficiently production Europe prioritize methods trait improvement coming years, need take into account socio‐economic, technological developments, including numerous policy socio‐economic challenges constraints. Based on wide range possible trends key uncertainties, developed four extreme learning scenarios that depict complementary developments. Here, elaborate how could inform direct research, aim highlight approaches be most promising or appropriate within each these world scenarios. Moreover, discuss some technology options would further needs multiple scenarios, such as improving breeding genetic engineering. In addition, diverse platforms food may offer unrealized potential, underutilized terrestrial aquatic species alternative sources nutrition biomass production. We demonstrate although several traits facilitate more efficient system others great potential all Altogether, this indicates depending which are heading toward, distinct fields should given priority feed decades.

Язык: Английский

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Climatic and stress memory in trees - and how to study it

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 399 - 418

Опубликована: Янв. 1, 2025

Язык: Английский

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Genetically modified trees (concept, principles, and applications)

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 357 - 371

Опубликована: Янв. 1, 2025

Язык: Английский

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The future of rubber production: a review of genetic regulation of biosynthetic pathways and genome editing technologies in natural rubber-producing plants

Tree Genetics & Genomes, Год журнала: 2025, Номер 21(2)

Опубликована: Фев. 13, 2025

Язык: Английский

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Photosynthetic Adaptation in Poplar Under Abiotic and Biotic Stress: Integrating Molecular, Physiological, and Biotechnological Perspectives

International Journal of Plant Biology, Год журнала: 2025, Номер 16(2), С. 42 - 42

Опубликована: Апрель 1, 2025

In the context of global climate change, carbon storage and sequestration capacity terrestrial ecosystems is increasing concern. Poplars are widely planted because their fast growth environmental adaptability. We reviewed effects abiotic biotic stresses on photosynthesis in poplar, focusing damage caused by adversity conditions to photosynthetic apparatus, which leads decreased dioxide (CO2) assimilation an increase reactive oxygen species (ROS)-induced oxidative damage. The mechanisms response stress poplar reviewed, especially role genes regulation regulating efficiency. These findings particularly important for improving resilience under changing conditions. addition, we discussed a range strategies enhance stress, such as genetic engineering synthetic biology. approaches provide theoretical guidance insights other crops facing similar challenges.

Язык: Английский

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