Antioxidants,
Год журнала:
2024,
Номер
13(12), С. 1474 - 1474
Опубликована: Ноя. 29, 2024
Bone
marrow
mesenchymal
stem
cells
(BM-MSCs)
play
a
crucial
role
in
bone
formation
through
their
ability
to
differentiate
into
osteoblasts.
Aging,
however,
detrimentally
affects
the
differentiation
and
proliferation
capacities
of
BM-MSCs,
consequently
impairing
regeneration.
Thus,
mitigating
aging
effects
on
BM-MSCs
is
vital
for
addressing
bone-related
pathologies.
In
this
study,
we
demonstrate
that
extracellular
nanovesicles
isolated
from
gold
kiwi
(GK-NVs)
protect
human
ultraviolet
(UV)-induced
photoaging,
thereby
alleviating
aging-related
impairments
cellular
functions
are
homeostasis.
Notably,
GK-NVs
were
efficiently
taken
up
by
without
causing
cytotoxicity.
reduced
intracellular
reactive
oxygen
species
(ROS)
levels
upon
UV
irradiation,
restoring
impaired
migration
capabilities.
Furthermore,
corrected
skewed
UV-irradiated
enhancing
osteoblast
differentiation,
as
evidenced
increased
expression
osteoblast-specific
genes
calcium
deposition,
reducing
adipocyte
indicated
decreased
lipid
droplet
formation.
These
findings
position
promising
biomaterial
treatment
diseases
such
osteoporosis.
Processes,
Год журнала:
2024,
Номер
12(12), С. 2938 - 2938
Опубликована: Дек. 23, 2024
Plant-derived
extracellular
vesicles
(PDEVs)
are
lipid
bilayer
nanoparticles,
naturally
produced
by
plant
cells,
with
sizes
ranging
from
50
to
500
nm.
Recent
studies
have
highlighted
their
great
potential
in
the
biotechnological
and
medical
fields,
due
natural
origin,
high
biocompatibility
intrinsic
therapeutic
properties.
PDEVs
contain
a
complex
biological
cargo
of
proteins,
lipids,
nucleic
acids
secondary
metabolites,
including
antioxidants
anti-inflammatory
molecules,
making
them
ideal
for
biomedical
applications
such
as
drug
delivery.
These
play
key
role
intercellular
communication
gene
regulation,
proving
be
particularly
promising
personalized
medicine.
ability
improve
stability
bioavailability,
optimizing
targeted
release
minimizing
side
effects.
Despite
some
challenges,
compositional
variability
need
standardized
protocols,
at
gunsight
innovative
research
aimed
improving
loading
capacity
specificity.
This
review
aims
provide
comprehensive
overview
PDEVs,
exploring
structure,
isolation
methods,
functional
characteristics,
applications,
highlighting
advantages
over
synthetic
nanoparticles
animal-derived
vesicles,
leading
an
sustainable
solution
development
new
approaches.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Дек. 23, 2024
Plant-driven
extracellular
vesicles
(PEVs)
have
attracted
significant
interest
due
to
their
natural
origin,
remarkable
bioactivity,
and
efficacy
in
drug
encapsulation
target
delivery.
In
our
work,
from
Citri
Reticulate
Pericranium
(CEVs)
were
isolated
investigated
physicochemical
characteristics
biological
activities.
We
identified
the
vesicle
structures
as
regular,
with
a
particle
size
of
approximately
200
nm.
also
detected
large
quantities
lipids,
proteins,
carbohydrates,
well
total
phenols
flavonoids.
The
rich
content
CEVs
endows
them
antioxidant
anti-inflammatory
effects,
which
can
notably
increase
levels
GSH
effectively
promote
activity
markers
such
SOD,
CAT,
GR.
Additionally,
they
inhibit
level
inflammatory
like
NO
factors
(
IL-6
,
IL-1β
TNF-α
).
addition,
we
successfully
synthesized
EVs-nob
nanoparticles
83.75%
±
2.83%
rate
2.79%
0.02%
loading,
may
enhance
effects
nobiletin.
Our
research
provides
critical
insights
into
bioactivity
demonstrates
potential
PEVs
nanocarrier
creation,
thereby
promoting
advancement
more
for
biomedical
applications.
Toxics,
Год журнала:
2024,
Номер
12(9), С. 653 - 653
Опубликована: Сен. 5, 2024
Polycyclic
aromatic
hydrocarbons
(PAHs)
are
persistent
organic
pollutants
that
pose
significant
environmental
and
health
risks.
These
compounds
originate
from
both
natural
phenomena,
such
as
volcanic
activity
wildfires,
anthropogenic
sources,
including
vehicular
emissions,
industrial
processes,
fossil
fuel
combustion.
Their
classification
carcinogenic,
mutagenic,
teratogenic
substances
link
them
to
various
cancers
disorders.
PAHs
categorized
into
low-molecular-weight
(LMW)
high-molecular-weight
(HMW)
groups,
with
HMW
exhibiting
greater
resistance
degradation
a
tendency
accumulate
in
sediments
biological
tissues.
Soil
serves
primary
reservoir
for
PAHs,
particularly
areas
of
high
creating
substantial
risks
through
ingestion,
dermal
contact,
inhalation.
Coastal
aquatic
ecosystems
especially
vulnerable
due
concentrated
human
activities,
PAH
persistence
disrupting
microbial
communities,
inhibiting
plant
growth,
altering
ecosystem
functions,
potentially
leading
biodiversity
loss.
In
plants,
contamination
manifests
form
abiotic
stress,
inducing
oxidative
cellular
damage,
growth
inhibition.
Plants
respond
by
activating
antioxidant
defenses
stress-related
pathways.
A
notable
aspect
defense
mechanisms
involves
plant-derived
extracellular
vesicles
(PDEVs),
which
membrane-bound
nanoparticles
released
cells.
PDEVs
play
crucial
role
enhancing
facilitating
intercellular
communication
coordinating
responses.
The
interaction
between
PDEVs,
while
not
fully
elucidated,
suggests
complex
interplay
mechanisms.
may
contribute
detoxification
pollutant
sequestration
or
delivering
enzymes
capable
degradation.
Studying
provides
valuable
insights
stress
resilience
offers
potential
new
strategies
mitigating
PAH-induced
plants
ecosystems.
Horticulturae,
Год журнала:
2024,
Номер
10(5), С. 477 - 477
Опубликована: Май 7, 2024
Horticultural
plant
material
offers
several
advantages
for
isolating
exosomes
and
other
natural
plant-derived
exosome-like
nanoparticles
(PDENs)
due
to
the
accessibility
affordability
of
widespread
applications.
This
study
aims
explore
impacts
tomato
genotype
(‘Admiro’,
‘Roma’,
‘Brooklyn’,
‘Marmande’
‘Betalux’)
main
cultivation
parameters
in
controlled
environment
agriculture
on
yield
properties
their
PDENs
pharmaceutical
cosmeceutical
The
PDEN
yield,
size
distribution,
antioxidative
young
seedlings
were
evaluated.
‘Betalux’
was
distinguished
by
a
remarkably
higher
nanoparticle
concentration
uniform
distribution
selected
further
experiments.
impact
temperature
(18,
22,
26
°C),
nitrogen
nutrition
(0,
250,
500
mg
L−1),
lighting
photosynthetic
photon
flux
density
(PPFD;
150,
450
µmol
m−2
s−1)
investigated.
Optimal
conditions
consisting
22
°C,
250
L−1
nutrition,
s−1
PPFD
used
as
reference.
temperature,
intensity
resulted
highest
most
particle
preparations
keratinocyte
metabolic
activity.
Deviation
from
optimal
reduced
biomass
protein
yield.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(11), С. 6177 - 6177
Опубликована: Июнь 4, 2024
Morus
sp.
(mulberry)
has
a
long
tradition
of
use
as
medicinal
treatment,
including
for
cardiovascular
disease
and
type
2
diabetes,
being
shown
to
have
antioxidant
properties
promote
wound
healing.
Extracellular
vesicles
(EVs)
are
sub-micron,
membrane-enclosed
particles
that
were
first
identified
in
mammalian
bodily
fluids.
EV-like
been
described
plants
(PDVs)
similar
characteristics
EVs.
We
hypothesised
some
the
health
benefits
previously
attributed
fruit
could
be
due
release
PDVs.
isolated
PDVs
from
nigra
alba
via
ultracentrifugation
incubated
THP-1
monocytes,
differentiated
macrophages,
or
HMEC-1
endothelial
cells
with
pro-oxidant
compounds
DMNQ
(THP-1)
glucose
oxidase
(HMEC-1)
lipopolysaccharide
(LPS)
presence
different
fractions
mulberry
Mulberry
EVs
augmented
ROS
production
caused
downregulation
HMEC-1.
increased
LPS-induced
IL-1β
secretion
but
reduced
CCL2
TGF-β
macrophages.
In
scratch
assays,
inhibited
migration
proliferation
both
low
high
serum
conditions,
suggesting
they
opposing
effects
these
two
important
aspects
One
limitations
plant-derived
therapeutics
overcoming
bioavailability
compounds.
propose
provide
link
between
physiological
dose
therapeutic
benefit
by
protecting
plant
active
GIT
well
potentially
delivering
genetic
material
proteins
contribute
observed
benefits.
Plant-derived
extracellular
vesicles
(EVs)
are
promising
therapeutic
agents
owing
to
their
natural
abundance,
accessibility,
and
unique
biological
properties.
This
review
provides
a
comprehensive
exploration
of
the
potential
plant-derived
EVs
emphasizes
anti-inflammatory,
antimicrobial,
tumor-inhibitory
effects.
Here,
we
discussed
advancements
in
isolation
purification
techniques,
such
as
ultracentrifugation
size-exclusion
chromatography,
which
critical
for
maintaining
functional
integrity
these
nanovesicles.
Next,
investigated
diverse
administration
routes
carefully
weighed
respective
advantages
challenges
related
bioavailability
patient
compliance.
Moreover,
elucidated
multifaceted
mechanisms
action
EVs,
including
roles
anti-inflammation,
antioxidation,
antitumor
activity,
modulation
gut
microbiota.
We
also
impact
on
specific
diseases
cancer
inflammatory
bowel
disease,
highlighting
importance
addressing
current
production
scalability,
regulatory
compliance,
immunogenicity.
Finally,
proposed
future
research
directions
optimizing
EV
extraction
developing
targeted
delivery
systems.
Through
efforts,
envision
seamless
integration
into
mainstream
medicine,
offering
safe
potent
alternatives
across
various
medical
disciplines.
