Novel tumor-associated macrophage populations and subpopulations by single cell RNA sequencing

Frontiers in Immunology, Год журнала: 2024, Номер 14

Опубликована: Янв. 29, 2024

Tumor-associated macrophages (TAMs) are present in almost all solid tumor tissues. 16They play critical roles immune regulation, angiogenesis, stem cell activation, invasion and metastasis, resistance to therapy. However, it is unclear how TAMs perform these functions. With the application of single-cell RNA sequencing (scRNA-seq), has become possible identify TAM subpopulations associated with distinct In this review, we discuss four novel tumors based on core gene signatures by scRNA-seq, including FCN1 + , SPP1 C1Q CCL18 TAMs. Functional enrichment expression scRNA-seq data from different tissues found that may induce inflammation; potentially involved cancer whereas participate regulation suppression; And cells terminal immunosuppressive not only have a stronger function but also enhance metastasis. can be further divided into populations Meanwhile, will emerging evidence highlighting separating macrophage there exist potential disconnects between types identified their actual function.

Язык: Английский

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Glycyrrhizic Acid-Lipid Framework Nanovehicle Loading Triptolide for Combined Immunochemotherapy

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(35), С. 41337 - 41350

Опубликована: Авг. 24, 2023

Despite the acknowledged advantages of combined immunochemotherapy for tumor treatment, high efficiency co-delivery these agents into targeted tissue is still challenging. Herein, based on a "three-birds-with-one-stone" strategy, facile glycyrrhizic acid (GL)-lipid hybrid nanoplatform loading triptolide (TP/GLLNP) designed to better address dilemma. Differing from traditional liposomes with dual-drug NPs, GL cholesterol-like structure primarily employed construct lipid membrane skeleton GL-based nanoparticle (GLLNP), and then (TP) readily loaded in bilayer GLLNP. The fabricated GLLNP possessed similar drug efficacy, particle size, storage stability; none hemolysis; even higher fluidity; lower absorbed opsonin proteins compared conventional liposomes. Compared TP-loaded (TP/Lipo), TP/GLLNP exhibits significantly enhanced cellular uptake, cytotoxicity, apoptosis HepG2 cells. In addition, could ameliorate immunosuppression by promoting tumor-associated macrophage polarization M2 M1 phenotype. Furthermore, retention accumulation area be found. As expected, displayed synergistic anti-hepatocellular carcinoma efficacy vivo. conclusion, this study provides an inspirational strategy combine anti-HCC benefits TP using novel nanosystem.

Язык: Английский

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Lysine lactylation (Kla) might be a novel therapeutic target for breast cancer

BMC Medical Genomics, Год журнала: 2023, Номер 16(1)

Опубликована: Ноя. 10, 2023

Abstract Background Histone lysine lactylation ( Kla ) is a newly identified histone modification, which plays crucial role in cancer progression. Hence, we determined the prognostic value of breast (BC). Methods We obtained RNA expression profiles BC from The Cancer Genome Atlas (TCGA), following screening out Kla-specific genes. Furthermore, by constructing cox model based on Subsequently, lactate accumulation-related genes and through Human Protein HPA ), further performed correlation analysis their expression. Meanwhile, explored effects tumor microenvironment (TME), drug therapy immunotherapy. Moreover, predicted pathways influenced via gene set enrichment (GSEA). Results A total 1073 samples 112 normal controls were TCGA, 23 removed owing to inadequate clinical information. 257 differentially expressed DEKlaG s) BC. involved with CCR7 , IGFBP6 NDUFAF6 OVOL1 SDC1 was established, risk score could be visualized as an independent biomarker for remarkably associated immune microenvironment, related activation various BC-related KEGG pathways. Conclusion In conclusion, contributes resistance undesirable responses, prognosis, suggesting that expected new therapeutic target

Язык: Английский

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Immunogenic cell death inducers for cancer therapy: An emerging focus on natural products

Phytomedicine, Год журнала: 2024, Номер 132, С. 155828 - 155828

Опубликована: Июнь 13, 2024

Язык: Английский

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Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell Proliferation

OncoTargets and Therapy, Год журнала: 2024, Номер Volume 17, С. 113 - 128

Опубликована: Фев. 1, 2024

Purpose: Chronic gastroesophageal reflux disease (GERD) causes the abnormal of acid and bile salts, which would induce Barrett's esophagus (BE) esophageal adenocarcinoma (EAC). EGFR, as one main components exosome, plays an important role in cancer progression. Here, we investigated acidic salts (ABS)-induced exosomal EGFR EAC cell proliferation. Methods: Electronic microscopic examination Western blot were used to identify exosomes. blot, siRNA transfection, enzyme-linked immunosorbent assay, qRT-PCR, viability detection, mouse xenograft tumor models, immunohistochemical staining performed study function ABS-induced Results: We found that ABS improved level normal human epithelial cells, BE BE-associated cells. The results confirmed serum-derived exosomes from healthy persons patients suffering GERD, with or without GERD. Moreover, line-derived was promote macrophage M2 polarization through PI3K-AKT pathway. co-incubation medium macrophages proliferation growth, depended on level. CCL18 identified most effective component by binding its receptor PITPNM3 vitro vivo. Conclusion: Our findings demonstrate regulates This provides insight into development. Keywords: progression, pro-oncogenic, PI3K/AKT pathway, CCL18,

Язык: Английский

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Anti-metastatic effects of AGS-30 on breast cancer through the inhibition of M2-like macrophage polarization

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 172, С. 116269 - 116269

Опубликована: Фев. 16, 2024

AGS-30, a new andrographolide derivative, showed significant anticancer and anti-angiogenic characteristics. However, its role in controlling macrophage polarization tumor immune response is unknown. Thus, the main goals of this study are to investigate how AGS-30 regulates it suppresses breast cancer metastasis. inhibited IL-4 IL-13-induced RAW 264.7 THP-1 macrophages into M2-like phenotype. did not affect LPS IFN-γ-induced M1-like macrophages. reduced mRNA expressions CD206, Arg-1, Fizz-1, Ym-1, VEGF, IL-10, MMP2, MMP9 concentration-dependent manner. In contrast, treatment at 5 μM polarization. The conditioned medium from increased 4T1 cell migration invasion, whereas these effects. xenograft mice, volume weight were without affecting body after receiving AGS-30. also lung liver metastasis, with STAT6, CD31, Ki67 protein expressions. Moreover, tumors had considerably fewer Arg-1 expression, but proportion iNOS expression treatment. Same results found tail vein metastasis model. conclusion, shows that inhibits growth probably through inhibiting Our findings suggest may be potential immunotherapeutic alternative for metastatic cancer.

Язык: Английский

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Self-Assembled Nanoparticles from Xie-Bai-San Decoction: Isolation, Characterization and Enhancing Oral Bioavailability

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 3405 - 3421

Опубликована: Апрель 1, 2024

Background: Natural nanoparticles have been found to exist in traditional Chinese medicine (TCM) decoctions. However, whether natural can influence the oral bioavailability of active compounds has not elucidated. Using Xie-Bai-San decoction (XBSD) as an example, purpose this study was isolate, characterize and elucidate mechanism (N-XBSD) XBSD, further explore main could be enhanced by N-XBSD. Methods: N-XBSD were isolated from investigated its characterization formation mechanism, evaluation ability enhance compounds. Results: The successfully with average particle size 104.53 nm, PDI 0.27 zeta potential − 5.14 mV. Meanwhile, all eight most presented Kukoamine B self-assemble mulberroside A or liquiritin form nanoparticles, respectively. And FT-IR HRMS results indicated possible binding ammonium group kukoamine phenolic hydroxyl liquiritin, established UPLC-MS/MS method accurate reliable met quantitative requirements. pharmacokinetic behaviors similar rats. Most notably, compared that free drugs, C max , AUC 0-∞ 0-t T 1/2 MRT values index higher N-XBSD, a slower plasma clearance rate Conclusion: major XBSD mainly distributed formed through self-assembly among obviously promote compounds, indicating decoctions play important role therapeutic effects. Keywords: enhancing bioavailability, comparative pharmacokinetics, UPLC-MS/MS,

Язык: Английский

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Physicochemical characterization and anti-angiogenesis activity of polysaccharides from Amauroderma rugosum, a medicinal and edible mushroom

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 274, С. 133478 - 133478

Опубликована: Июнь 26, 2024

Язык: Английский

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Role of macrophage polarization in heart failure and traditional Chinese medicine treatment

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 18, 2024

Heart failure (HF) has a severe impact on public health development due to high morbidity and mortality is associated with imbalances in cardiac immunoregulation. Macrophages, major cell population involved immune response inflammation, are highly heterogeneous polarized into M1 M2 types depending the microenvironment. macrophage releases inflammatory factors chemokines activate remove harmful substances, while anti-inflammatory inhibit overactive promote tissue repair. restrict each other maintain homeostasis. The dynamic balance of closely related Traditional Chinese Medicine (TCM) yin-yang theory, imbalance yin yang will result pathological state organism. Studies have confirmed that TCM produces positive effects HF by regulating polarization. This review describes critical role polarization fibrosis, angiogenesis electrophysiology course HF, as well potential mechanism regulation preventing treating thereby providing new ideas for clinical treatment scientific research design HF.

Язык: Английский

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Taohong Siwu Decoction enhances the chemotherapeutic efficacy of doxorubicin by promoting tumor vascular normalization

Phytomedicine, Год журнала: 2024, Номер 134, С. 155995 - 155995

Опубликована: Авг. 31, 2024

Язык: Английский

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