Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 29, 2024
Introduction
With
the
depletion
of
wild
Astragali
Radix
(WA)
resources,
imitated-wild
(IWA)
and
cultivated
(CA)
have
become
main
products
Radix.
However,
quality
differences
three
growth
patterns
(WA,
IWA,
CA)
different
years
not
been
fully
characterized,
leading
to
a
lack
necessary
scientific
evidence
for
their
use
as
substitutes
WA.
Methods
We
innovatively
proposed
multidimensional
evaluation
method
that
encompassed
traits,
microstructure,
cell
wall
components,
saccharides,
pharmacodynamic
compounds,
comprehensively
explain
variances
among
Results
discussion
Our
study
showed
IWA
WA
was
comparatively
similar,
including
indicators
such
apparent
color,
sectional
structure
odor,
thickness
phellem,
diameter
number
vessels,
morphology
phloem
xylem,
levels
ratios
cellulose,
hemicellulose,
lignin,
sucrose,
starch,
water-soluble
polysaccharides,
total-saponins.
content
starch
sorbose
in
CA
significantly
higher
than
WA,
total-flavonoids
were
lower
indicating
significant
between
Hence,
we
suggest
should
be
used
substitute
instead
CA.
As
planting
our
results
indicated
aged
1-32
could
divided
into
stages
according
change:
rapid
period
(1-5
years),
stable
(6-20
elderly
(25-32
years).
Among
these,
6-20
old
exhibited
consistent
comparative
results,
showcasing
elevated
key
active
components
flavonoids,
saponins.
Considering
both
cultivation
expenses
recommend
duration
6-8
growers.
In
conclusion,
established
novel
systematically
characterize
Radix,
provided
new
perspective
artificial
assurance
Pharmaceuticals,
Год журнала:
2023,
Номер
16(5), С. 659 - 659
Опубликована: Апрель 28, 2023
Astragalus
species
are
traditionally
used
for
diabetes,
ulcers,
leukemia,
wounds,
stomachaches,
sore
throats,
abdominal
pain,
and
toothaches.
Although
the
preventive
effects
of
against
diseases
known,
there
is
no
record
therapeutic
alopecurus.
In
this
study,
we
aimed
to
evaluate
in
vitro
antiglaucoma,
antidiabetic,
anti-Alzheimer's
disease,
antioxidant
activities
methanolic
(MEAA)
water
(WEAA)
extracts
aerial
part
A.
Additionally,
its
phenolic
compound
profiles
were
analyzed
by
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS).
MEAA
WEAA
evaluated
their
inhibition
ability
on
α-glycosidase,
α-amylase,
acetylcholinesterase
(AChE),
human
carbonic
anhydrase
II
(hCA
II)
enzymes.
The
compounds
LC-MS/MS.
Furthermore,
total
flavonoid
contents
determined.
context,
activity
was
1,1-diphenyl-2-picrylhydrazyl
(DPPH),
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid)
(ABTS),
N,N-dimethyl-p-phenylene
diamine
(DMPD),
ferric
reducing
power
(FRAP),
cupric
ions
(Cu2+)
capacity
(CUPRAC),
(Fe3+)
reducing,
ferrous
(Fe2+)
chelating
methods.
had
IC50
values
9.07
2.24
μg/mL
693.15
346.58
1.99
2.45
AChE,
147.7
171.7
hCA
II.
While
amounts
16.00
18.50
μg
gallic
acid
equivalent
(GAE)/mg
extract,
both
calculated
as
66.23
33.115
quercetin
(QE)/mg,
respectively.
showed,
respectively,
variable
DPPH
radical
scavenging
(IC50:
99.02
115.53
μg/mL),
ABTS
32.21
30.22
µg/mL),
DMPD
231.05
65.22
Fe2+
46.21
33.01
μg/mL).
abilities
were,
Fe3+
(λ700:
0.308
0.284),
FRAP
(λ593:
0.284
CUPRAC
(λ450:
0.163
0.137).
A
35
phenolics
scanned,
10
determined
LC-MS/MS
analysis.
revealed
that
mainly
contained
isorhamnetin,
fumaric
acid,
rosmarinic
derivatives.
This
first
report
indicating
have
abilities,
activities.
These
results
demonstrate
potential
through
properties
enzyme
inhibitor
medicine.
work
provides
foundation
further
research
into
establishment
novel
therapeutics
glaucoma,
Alzheimer's
disease.
Ultrasonics Sonochemistry,
Год журнала:
2023,
Номер
98, С. 106522 - 106522
Опубликована: Июль 10, 2023
The
water
extraction
and
ethanol
precipitation
method
is
an
based
on
the
solubility
characteristics
of
polysaccharides
that
offers
wide
applicability
in
separation
plant
polysaccharides.
However,
this
leads
to
large
amounts
proteins,
nucleic
acids,
pigments,
other
impurities
products,
which
makes
downstream
purification
complicated
time-consuming.
In
study,
a
green,
high-density
natural
deep
eutectic
solvents
was
used
for
high-purity
from
Astragalus
membranaceus
(Fisch)
Bge.
var.
Mongholicus
(Bge.)
Hsiao
roots
under
ultrasound-assisted
conditions.
16
different
were
designed
screen
best
solvent
extracting
(APSs).
Based
yield
recovery
APSs,
composed
choline
chloride
oxalic
acid
with
molar
ratio
1:2
selected.
related
factors
affecting
investigated.
To
improve
operating
methodology,
single-factor
trials,
Plackett-Burman
design,
Box-Behnken
design
used.
optimal
process
conditions
obtained
as
follows:
content
55%,
liquid-solid
24
mL/g,
ultrasonic
irradiation
time
54
min,
temperature
50
°C,
power
480
W,
h,
concentration
75%.
Under
conditions,
APSs
61.4
±
0.6
mg/g.
Considering
special
matrix
A.
roots,
physical-technology-based
waves
promote
penetration,
mass
transfer
function
also
solves
bottleneck
high-viscosity
stage.
comparison
conventional
method,
proposed
isolation
can
significantly
increase
recovery,
beneficial
simplifying
by
using
properties
separate
extracts
reduce
APSs.
Archiv der Pharmazie,
Год журнала:
2024,
Номер
357(9)
Опубликована: Июнь 7, 2024
The
n-hexane,
ethyl
acetate,
ethanol,
ethanol/water
(70%
ethanol),
and
water
extracts
of
Astragalus
aduncus
aerial
parts
were
investigated
for
their
antioxidant
potential,
enzyme
inhibition
activity
(anti-acetylcholinesterase
[AChE],
anti-butyrylcholinesterase
[BChE],
antityrosinase,
antiamylase,
antiglucosidase)
antiproliferative
effect
(against
colon
adenocarcinoma
cell
line
[HT-29],
gastric
cancer
[HGC-27],
prostate
carcinoma
[DU-145],
breast
[MDA-MB-231],
cervix
[HeLa]).
In
addition,
the
phytochemical
profile
was
evaluated
using
validated
spectrophotometric
high-pressure
liquid
chromatography-electrospray
ionization/tandem
mass
spectroscopy
methods.
Generally,
70%
ethanol
extract
demonstrated
strongest
properties,
it
richest
source
total
phenolic
constituents.
Our
findings
indicated
that
acetate
most
potent
BChE
inhibitor
(11.44
mg
galantamine
equivalents
[GALAE]/g)
followed
by
(8.51
GALAE/g),
while
promising
AChE
(3.42
GALAE/g)
(3.17
GALAE/g).
Excellent
tyrosinase
inhibitory
(66.25
kojic
acid
equivalent/g)
observed
in
part
A.
aduncus.
Тhese
results
showed
cytotoxic
effects
exhibited
against
HGC-27
cells
(IC
PLoS ONE,
Год журнала:
2025,
Номер
20(2), С. e0319067 - e0319067
Опубликована: Фев. 25, 2025
Background
Doxorubicin
(DOX)
is
a
powerful
chemotherapeutic
agent
commonly
employed
in
cancer
treatment.
However,
its
clinical
utility
constrained
by
dose-dependent
cardiotoxicity,
which
can
result
heart
failure
and
sudden
cardiac
death.
The
molecular
mechanisms
of
DOX-induced
cardiotoxicity
(DIC)
include
oxidative
stress,
mitochondrial
dysfunction,
the
activation
cell
death
pathways,
including
ferroptosis.
There
an
urgent
need
for
effective
therapeutic
strategies
to
mitigate
DIC.
Methods
This
study
investigates
cardioprotective
effects
Astragalus
Polyphenols
(ASP),
bioactive
compound
extracted
from
membranaceus.
In
context
DIC,
we
utilized
AC16
H9C2
cardiomyocytes
establish
DIC
model
assessed
ASP
on
viability,
function,
PI3K/AKT/NRF2
signaling
pathway.
expression
atrial
natriuretic
peptide
(ANP)
brain
(BNP),
markers
injury,
was
also
evaluated.
Results
treatment
significantly
reversed
reductions
viability
membrane
potential
(MMP)
while
decreasing
levels
reactive
oxygen
species
(ROS).
Additionally,
downregulated
ANP
BNP,
indicating
protective
effect
cardiomyocytes.
Furthermore,
activated
pathway,
suppressed
DOX.
Inhibition
this
pathway
using
LY294002
ML385
abolishes
ASP,
suggesting
that
mediates
through
axis.
Conclusion
exhibits
against
regulating
reduce
stress
preserve
function.
These
findings
suggest
may
serve
as
alleviate
Our
results
provide
novel
strategy
protect
patients
undergoing
DOX
chemotherapy.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(2), С. 1235 - 1235
Опубликована: Янв. 19, 2024
Keloid
is
a
disease
in
which
fibroblasts
abnormally
proliferate
and
synthesize
excessive
amounts
of
extracellular
matrix,
including
collagen
fibronectin,
during
the
healing
process
skin
wounds,
causing
larger
scars
that
exceed
boundaries
original
wound.
Currently,
surgical
excision,
cryotherapy,
radiation,
laser
treatment,
photodynamic
therapy,
pressure
silicone
gel
sheeting,
pharmacotherapy
are
used
alone
or
combinations
to
treat
this
disease,
but
outcomes
usually
unsatisfactory.
The
purpose
review
examine
whether
natural
products
can
help
keloid
disease.
I
introduce
well-established
therapeutic
targets
for
various
other
emerging
have
been
proposed
based
on
phenotypic
difference
between
keloid-derived
(KFs)
normal
epidermal
(NFs).
We
then
present
recent
studies
biological
effects
plant-derived
extracts
compounds
KFs
NFs.
Associated
ex
vivo,
clinical
also
presented.
Finally,
we
discuss
mechanisms
action
compounds,
pros
cons,
future
tasks
product-based
therapy
as
compared
with
existing
therapies.
Extracts
Astragalus
membranaceus,
Salvia
miltiorrhiza,
Aneilema
keisak,
Galla
Chinensis,
Lycium
chinense,
Physalis
angulate,
Allium
sepa,
Camellia
sinensis
appear
modulate
cell
proliferation,
migration,
and/or
matrix
(ECM)
production
KFs,
supporting
their
potential.
Various
phenolic
terpenoids,
alkaloids,
could
different
signaling
pathways
associated
pathogenesis
keloids.
For
now,
many
limited
vitro
experiments;
additional
research
development
needed
proceed
trials.
Many
accelerate
discovery
substances
prevention
treatment
hope
will
bridge
past,
present,
subject
provide
insight
into
new
pharmaceuticals,
aiming
effective
treatment.
