Corneal stromal repair and regeneration

Progress in Retinal and Eye Research, Год журнала: 2022, Номер 91, С. 101090 - 101090

Опубликована: Май 29, 2022

Язык: Английский

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Bioprinting of human pluripotent stem cell derived corneal endothelial cells with hydrazone crosslinked hyaluronic acid bioink

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Март 14, 2024

Abstract Background Human corneal endothelial cells lack regenerative capacity through cell division in vivo . Consequently, the case of trauma or dystrophy, only available treatment modality is tissue primary transplantation from cadaveric donor which faces a high global shortage. Our ultimate goal to use state-of-the-art 3D-bioprint technology for automated production human partial and full-thickness tissues using stem functional bioinks. In this study, we explore feasibility bioprinting endothelium pluripotent derived hydrazone crosslinked hyaluronic acid bioink. Methods Corneal differentiated were bioprinted optimized based Before process, biocompatibility bioink with was first analyzed on ex denuded rat porcine corneas as well Descemet membrane. Subsequently, proceeded viability verified live/dead stainings. Histological immunofluorescence stainings involving ZO1, Na + /K -ATPase CD166 used confirm phenotype all experiments. Additionally, STEM121 marker identify corneas. Results The bioink, modified successfully supported both printability cells. Following up 10 days transplantations, positive confirmed membrane cornea demonstrating Furthermore, validated showing -like characteristics. Seven post bioprinting, viable showed polygonal morphology expression native-like localization ZO-1, CD166. However, mesenchymal-like observed certain areas cultures, spreading beneath endothelial-like layer. Conclusions results demonstrate successful printing covalently This approach not holds promise transplants but also presents potential applications broader mission cornea.

Язык: Английский

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Advancements in bioengineering for descemet membrane endothelial keratoplasty (DMEK)

npj Regenerative Medicine, Год журнала: 2025, Номер 10(1)

Опубликована: Фев. 14, 2025

Abstract Corneal diseases are the third leading cause of blindness worldwide. Descemet’s Membrane Endothelial Keratoplasty (DMEK) is preferred surgical technique for treating corneal endothelial disorders, relying heavily on high-quality donor tissue. However, scarcity suitable tissue and sensitivity cells remain significant challenges. This review explores current state DMEK, focusing advancements in engineering as a promising solution to improve outcomes address limitations.

Язык: Английский

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Silk biomaterials for corneal tissue engineering: From research approaches to therapeutic potentials; A review

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141039 - 141039

Опубликована: Фев. 15, 2025

Язык: Английский

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Single cell transcriptomics reveals the heterogeneity of the human cornea to identify novel markers of the limbus and stroma

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Ноя. 5, 2021

Abstract The cornea is the clear window that lets light into eye. It composed of five layers: epithelium, Bowman’s layer, stroma, Descemet’s membrane and endothelium. maintenance its structure transparency are determined by functions different cell types populating each layer. Attempts to regenerate corneal tissue understand disease conditions requires knowledge how profiles vary across this heterogeneous tissue. We performed a single transcriptomic profiling 19,472 cells isolated from eight healthy donor corneas. Our analysis delineates heterogeneity layers identifying populations revealing states contribute in preserving homeostasis. identified expression CAV1 , HOMER3 CPVL epithelial limbal stem niche, CKS2 STMN1 UBE2C were exclusively expressed highly proliferative transit amplifying cells, CXCL14 was suprabasal/superficial limbus, NNMT stromal keratocytes. Overall, research provides basis improve current primary expansion protocols, for future states, help guide pluripotent lineages, engineered substrates affect regenerative therapies.

Язык: Английский

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Lentiviral Vectors for Ocular Gene Therapy

Pharmaceutics, Год журнала: 2022, Номер 14(8), С. 1605 - 1605

Опубликована: Июль 31, 2022

This review offers the basics of lentiviral vector technologies, their advantages and pitfalls, an overview use in field ophthalmology. First, description global challenges encountered to develop safe efficient recombinant vectors for clinical application is provided. The risks measures taken minimize secondary effects as well new strategies using these are also discussed. then focuses on specifically designed ocular therapy goes over preclinical studies describing safety efficacy. A therapeutic approach vector-mediated gene currently being developed many diseases, e.g., aged-related macular degeneration, retinopathy prematurity, inherited retinal dystrophies (Leber congenital amaurosis type 2, Stargardt disease, Usher syndrome), glaucoma, corneal fibrosis or engraftment rejection. In summary, this shows how offer interesting alternative all compartments.

Язык: Английский

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Cell therapy in the cornea: The emerging role of microenvironment

Progress in Retinal and Eye Research, Год журнала: 2024, Номер 102, С. 101275 - 101275

Опубликована: Май 24, 2024

Язык: Английский

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An Osteoimmunomodulatory Biopatch Potentiates Stem Cell Therapies for Bone Regeneration by Simultaneously Regulating IL‐17/Ferroptosis Signaling Pathways

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Июль 18, 2024

Currently, there are still great challenges in promoting bone defect healing, a common health problem affecting millions of people. Herein an osteoimmunity-regulating biopatch capable stem cell-based therapies for regeneration is developed. A totally biodegradable conjugate first synthesized, which can self-assemble into bioactive nano micelles (PPT NMs). This nanotherapy effectively improves the osteogenesis periodontal ligament cells (PDLSCs) under pathological conditions, by simultaneously regulating IL-17 signaling and ferroptosis pathways. Incorporation PPT NMs electrospun nanofibers affords patch, notably formation two rat models. Janus bio patch then engineered integrating with cell sheet PDLSCs. The obtained shows additionally potentiated capacity, synergistically osteoimmune microenvironment facilitating differentiation. Further surface functionalization tannic acid considerably increases its adhesion to defect, prolongs local retention, sustains bioactivities, thereby offering much better repair effects rats mandibular or cranial defects. Moreover, patches display good safety. Besides defects, this strategy be applied promote spinal cord injury, wound skin burns.

Язык: Английский

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Human SMILE-Derived Stromal Lenticule Scaffold for Regenerative Therapy: Review and Perspectives

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(14), С. 7967 - 7967

Опубликована: Июль 19, 2022

A transparent cornea is paramount for vision. Corneal opacity one of the leading causes blindness. Although conventional corneal transplantation has been successful in recovering patients’ vision, outcomes are challenged by a global lack donor tissue availability. Bioengineered tissues gaining momentum as new source wound healing and scar management. Extracellular matrix (ECM)-scaffold-based engineering offers perspective on regenerative medicine. Ultrathin stromal laminar obtained from lenticule-based refractive correction procedures, such SMall Incision Lenticule Extraction (SMILE), an accessible novel collagen-rich ECM scaffolds with high mechanical strength, biocompatibility, transparency. After customization (including decellularization), these lenticules can serve acellular scaffold niche to repopulate cells, including keratocytes stem functional phenotypes. The intrastromal cell/tissue composites regenerate native-like restore This review highlights current status ECM-scaffold-based along development drug growth factor delivery systems, elucidates potential uses lenticule therapeutics.

Язык: Английский

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Animal Models in Eye Research: Focus on Corneal Pathologies

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(23), С. 16661 - 16661

Опубликована: Ноя. 23, 2023

The eye is a complex sensory organ that enables visual perception of the world. dysfunction any these tissues can impair vision. Conduction studies on laboratory animals are essential to ensure safety therapeutic products directly applied or injected into treat ocular diseases before eventually proceeding clinical trials. Among tissues, cornea has unique homeostatic and regenerative mechanisms for maintaining transparency refraction external light, which However, being outermost tissue exposed environment, particularly susceptible injury diseases. This review highlights evidence selecting appropriate better understand corneal diseases, rank as fifth leading cause blindness worldwide. development reliable human-relevant animal models is, therefore, valuable research tool understanding translating fundamental mechanistic findings, well assessing potential in humans. First, this emphasizes characteristics used research. Subsequently, it discusses current associated with human pathologies, their utility disease mechanisms, role translational patients.

Язык: Английский

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