Acta Neurobiologiae Experimentalis,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 30, 2024
MicroRNA‑regulated
gene
expression
plays
an
important
role
in
autoimmune
diseases,
such
as
multiple
sclerosis
(MS).
This
study
investigated
the
patterns
of
microRNAs
(miRNAs)
MS
brain
tissues
using
animal
experimental
encephalomyelitis
(EAE)
model
treated
with
Hypericum
perforatum
(HP)
oil.
C57BL/6
J
mice
were
divided
into
two
groups:
and
control.
The
group
was
subdivided
sham
(MS)
MS+HP.
After
EAE
induction
treatment
protocol,
miRNA
profiles
determined
samples
groups.
array
data
identified
eleven
miRNAs
candidate
validation
performed
by
RT‑qPCR.
A
literature
review
validated
found
that
six
(miR‑200a‑3p,
miR‑200b‑3p,
miR‑200c‑3p,
miR‑182‑5p,
miR‑183‑5p,
miR‑1298‑5p)
directly
associated
MS.
These
have
been
suggested
biomarkers
because
they
are
highly
correlated
pathology
disease.
Furthermore,
analysis
five
(miR‑299a‑5p,
miR‑206‑3p,
miR‑325‑5p,
miR‑10b‑5p,
miR‑429‑3p)
likely
to
be
pathogenesis,
which
could
helpful
diagnosis
research
offers
vital
insights
utilized
creating
advancing
treatments
for
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(4), С. 2255 - 2255
Опубликована: Фев. 13, 2024
Multiple
sclerosis
(MS)
is
a
degenerative
condition
characterized
by
axonal
damage
and
demyelination
induced
autoreactive
immune
cells
that
occur
in
the
Central
Nervous
System
(CNS).
The
interaction
between
epigenetic
changes
genetic
factors
can
be
widely
involved
onset,
development,
progression
of
disease.
Although
numerous
efforts
were
made
to
discover
new
therapies
able
prevent
improve
course
MS,
definitive
curative
treatments
have
not
been
found
yet.
However,
recent
years,
it
has
reported
non-coding
RNAs
(ncRNAs),
including
microRNAs
(miRNAs),
long
ncRNAs
(lncRNAs),
circular
(circRNAs),
acting
as
gene
expression
regulators,
could
used
potential
therapeutic
targets
or
biomarkers
diagnose
fight
MS.
In
this
review,
we
discussed
role
miRNAs,
lncRNAs,
circRNAs,
well
their
level
signaling
pathways
are
related
preclinical
human
MS
studies.
Hence,
investigation
important
provide
additional
information
regarding
pathogenesis
promote
discovery
strategies
biomarkers.
Immunity Inflammation and Disease,
Год журнала:
2023,
Номер
11(12)
Опубликована: Дек. 1, 2023
Abstract
Background
Autoimmune
diseases
(AD)
are
severe
pathophysiological
ailments
that
stimulated
by
an
exaggerated
immunogenic
response
towards
self‐antigens,
which
can
cause
systemic
or
site‐specific
organ
damage.
An
array
of
complex
genetic
and
epigenetic
facets
majorly
contributes
to
the
progression
AD,
thus
providing
significant
insight
into
regulatory
mechanism
microRNA
(miRNA).
miRNAs
short,
non‐coding
RNAs
have
been
identified
as
essential
contributors
post‐transcriptional
regulation
host
genome
expression
crucial
regulators
a
myriad
biological
processes
such
immune
homeostasis,
T
helper
cell
differentiation,
central
peripheral
tolerance,
development.
Aims
This
article
tends
deliberate
conceptualize
brief
pathogenesis
pertinent
well
miRNA
networks
affecting
five
different
ADs
namely
rheumatoid
arthritis
(RA),
type
1
diabetes,
multiple
sclerosis
(MS),
lupus
erythematosus
(SLE)
inflammatory
bowel
disorder
(IBD)
thereby
novel
miRNA‐based
theranostic
interventions.
Results
&
Discussion
Pertaining
differential
attributed
in
target
tissues
cellular
bodies
innate
adaptive
immunity,
paradigm
scientific
expeditions
suggests
optimistic
correlation
between
dysfunction
alterations.
Conclusion
Therefore,
it
is
not
astonishing
dysregulations
patterns
now
recognized
wide
spectrum
disorders,
establishing
themselves
potential
biomarkers
therapeutic
targets.
Owing
its
potencies,
targets
widely
utilized
development
biosensors
other
molecules
originating
from
same.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(10), С. 5412 - 5412
Опубликована: Май 15, 2024
Multiple
sclerosis
is
a
chronic
immune-mediated
disorder
of
the
central
nervous
system
with
high
heterogeneity
among
patients.
In
clinical
setting,
one
main
challenges
proper
and
early
diagnosis
for
prediction
disease
activity.
Current
based
on
integration
clinical,
imaging,
laboratory
results,
latter
presence
intrathecal
IgG
oligoclonal
bands
in
cerebrospinal
fluid
whose
detection
via
isoelectric
focusing
followed
by
immunoblotting
represents
gold
standard.
Intrathecal
synthesis
can
also
be
evidenced
measurement
kappa
free
light
chains
fluid,
which
has
reached
similar
diagnostic
accuracy
compared
to
that
identification
patients
multiple
sclerosis;
moreover,
recent
studies
have
highlighted
its
value
activity
prediction.
This
strategy
significant
advantages
as
using
band
detection,
even
though
some
issues
remain
open.
Here,
we
discuss
current
methods
applied
analysis
achieve
most
accurate
follow-up
prognosis
evaluation.
addition,
describe
new
promising
biomarkers,
currently
under
investigation,
could
contribute
both
better
monitoring
therapeutic
treatment
response.
Abstract
Multiple
sclerosis
(MS)
and
Neuromyelitis
Optica
(NMO)
are
immune-related
CNS
inflammatory
diseases
that
often
present
with
overlapping
clinical
symptoms,
leading
to
frequent
misdiagnosis,
particularly
in
aquaporin-4
seronegative
NMO
patients.
Identifying
the
underlying
mechanisms
of
these
is
critical
for
discovering
biomarkers
enable
timely
diagnosis
effective
treatment.
This
study
included
252
participants,
divided
into
four
groups.
Group
I
(Relapsing–Remitting
MS:
RRMS
group),
II
(Secondary
Progressive
SPMS
III
(NMO
IV
(Healthy
controls).
Blood
samples
were
collected
from
all
participants
measure
expression
levels
Neuregulin-1
(NRG1),
lncRNA
Ftx,
miR-382-5p
.
The
results
showed
NRG1
significantly
lower
RRMS,
SPMS,
groups
compared
healthy
controls,
most
pronounced
reduction
observed
NMO,
suggesting
may
serve
as
a
potential
biomarker
differentiating
MS,
especially
cases
where
traditional
diagnostic
criteria
inconclusive.
Ftx
,
sponge
exhibited
an
opposite
trend
was
downregulated
its
promising
biomarker.
Our
findings
highlight
NRG1,
diagnostic,
screening,
prognostic
biomarkers,
well
tools
differential
MS.
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