Egyptian Dental Journal /Egyptian Dental Journal,
Год журнала:
2024,
Номер
70(2), С. 1299 - 1309
Опубликована: Апрель 1, 2024
Background:
Cancer
is
the
leading
cause
of
death
in
developing
nations
after
cardiac
diseases.
Fighting
cancer
a
highly
challenging
task.
Natural
compounds
are
becoming
major
source
for
many
anti-cancer
drugs.
Camel
milk
well
known
its
benefits
to
human
health
and
believed
be
able
treat
several
ailments,
including
cancer.Aim:
The
current
study
was
designed
explore
at
possible
effects
camel
on
tongue
squamous
cell
carcinoma
line
(HNO-97
cells).Methods:
anticancer
effectiveness
assessed
using
viability
assay,
cytological
evaluation,
flow
cytometric
analysis,
nuclear
morphometric
western
blotting
technique,
statistical
analysis.
Results:
findings
demonstrated
that
had
dose-dependent
cytotoxic
impact
HNO-97
cells.
mainly
triggered
apoptosis
Nuclear
report
revealed
that,
mean
area
factor
values
treated
groups
were
less
than
those
control
group.
Regarding
caspase-3
P53
expression
levels
significantly
increased
groups.Conclusion:
considerably
effect
cells.Keywords:
milk,
line,
apoptosis,
technique.Ethical
Regulation:
All
investigations
included
conducted
compliance
with
ethical
guidelines
Research
Ethics
Committee
Faculty
Dentistry,
Minia
University,
Egypt,
approval
number
667
Tuesday,
27/12/2022.
Abstract
Background
The
oral
cavity
is
home
to
various
ecological
niches,
each
with
its
own
unique
microbial
composition.
Understanding
the
communities
and
gene
composition
in
different
niches
within
of
cancer
(OC)
patients
crucial
for
determining
how
these
populations
contribute
disease
progression.
Methods
In
this
study,
saliva
dental
plaque
samples
were
collected
from
OC.
Metagenomic
sequencing
was
employed
analyze
community
classification
functional
sample
groups.
Results
results
study
revealed
significant
differences
both
function
between
samples.
diversity
species
found
be
higher
compared
that
Notably,
Actinobacteria
enriched
OC
patients.
Furthermore,
identified
several
inter-group
differential
marker
species,
including
Prevotella
intermedia
,
Haemophilus
parahaemolyticus
Actinomyces
radius
Corynebacterium
matruchitii
Veillonella
atypica
.
Additionally,
1,353
genes
annotated
into
23
pathways.
Interestingly,
a
correlation
observed
differentially
labeled
Herpes
simplex
virus
1
(HSV-1)
infection,
which
may
related
occurrence
development
cancer.
Conclusions
Significant
genetic
pathogenic
bacteria
associated
diseases
predominantly
saliva.
identification
biomarkers
pathways
provide
insights
relationship
microbiota
