H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence

Biomaterials, Год журнала: 2024, Номер 311, С. 122659 - 122659

Опубликована: Июнь 7, 2024

Язык: Английский

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A novel senotherapeutic strategy with azithromycin for preventing endometriosis progression

Reproductive Biology and Endocrinology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 26, 2025

Endometriosis is an estrogen-dependent chronic inflammatory disease, however the mechanisms underlying inflammation remain unclear. Non-hormonal drugs that can prevent endometriosis progression and resolve endometriotic infertility are urgently required. We thus focused on cellular senescence as a novel feature of endometriosis. Senescent cells cause via senescence-associated secretory phenotype (SASP) factor. It has been reported effects senolysis for various diseases in recent years. The aim this study was to validate involvement senolytic drug develop non-hormonal therapeutic strategy markers were assessed by morphological features semiquantitative immunofluorescence staining (senescence-associated b-galactosidase [SA-b-Gal], cyclin-dependent kinase inhibitor 2 A locus [p16INK4a], laminB1) compare among cell types (normal endometrial stromal [nESCs], with [eESCs], ovarian [OE] cyst-derived [CSCs]). Expression SASP examined culture supernatants using cytokine array. In addition, (azithromycin [AZM] navitoclax [ABT263]) evaluated vitro vivo. vivo used mice model. CSCs exhibited stronger markers. Semi-quantitative SA-β-Gal p16INK4a intensities significantly increased, LaminB1 decreased compared those nESCs eESCs (SA-b-Gal, P < 0.001; p16INK4a, 0.05; LaminB1, 0.05). Cytokine array analysis revealed elevated SASP-related levels, including interleukin-6 (IL-6), CSC nESCs. AZM ABT263 reduced viable fraction (AZM: 0.001, ABT263: 0.01). Furthermore, suppressed IL-6 expression (P murine model, administration lesion volume vehicle Proliferative activity, fibrosis within lesions also (Ki67, 0.01; IL-6, fibrosis, 0.001). Our findings show involved pathogenesis may be useful preventing suppressing secretion SASP.

Язык: Английский

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Cellular oxidative stress and sirtuins mediate regulation of senescence and neuronal differentiation by withaferin A

Free Radical Biology and Medicine, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Phytocannabinoids Stimulate Rejuvenation and Prevent Cellular Senescence in Human Dermal Fibroblasts

Cells, Год журнала: 2022, Номер 11(23), С. 3939 - 3939

Опубликована: Дек. 6, 2022

In light of the increased popularity phytocannabinoids (pCBs) and their appearance in beauty products without rigorous research on rejuvenation efficacy, we decided to investigate potential role pCBs skin rejuvenation. Utilizing healthy stress-induced premature senescent (SIPS) CCD-1064Sk fibroblasts, effects cellular viability, functional activity, metabolic function, nuclear architecture were tested. Both delta-9-tetrahydrocannabinol (THC) cannabidiol (CBD) within range 0.5 µM 2.0 cell growth a dose-dependent manner while significantly decreasing senescence as measured by beta-galactosidase activity. scratch assay, both THC CBD (2.0 µM) improved wound healing SIPS fibroblasts. altered mRNA levels cycle regulators genes involved ECM production. Subsequently, found ELN, Cyclin D1, PCNA, BID protein but ameliorated after exposure human dermal Lastly, compared efficacy with common anti-aging nutrient signaling replicative adult CCD-1135Sk. improve better than metformin, rapamycin, triacetylresveratrol CCD-1135Sk Therefore, can be valuable source biologically active substances used cosmetics, more studies using clinical trials should performed confirm phytocannabinoids.

Язык: Английский

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Molecular mechanism of caloric restriction mimetics-mediated neuroprotection of age-related neurodegenerative diseases: an emerging therapeutic approach

Biogerontology, Год журнала: 2023, Номер 24(5), С. 679 - 708

Опубликована: Июль 10, 2023

Язык: Английский

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