Could Cytoplasmic Lipid Droplets be Linked to Inefficient Oxidative Phosphorylation in Cancer?

Current Tissue Microenvironment Reports, Год журнала: 2024, Номер 5(4), С. 109 - 117

Опубликована: Май 17, 2024

Язык: Английский

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O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study

World Journal of Diabetes, Год журнала: 2025, Номер 16(3)

Опубликована: Янв. 20, 2025

BACKGROUND Periodontitis, when exacerbated by diabetes, is characterized increased M1 macrophage polarization and decreased M2 polarization. O-linked β-N-acetylglucosamine (O-GlcNAcylation), catalyzed O-GlcNAc transferase (OGT), promotes inflammatory responses in diabetic periodontitis (DP). Additionally, p38 mitogen-activated protein kinase regulates However, the interplay between OGT, polarization, signaling progression of DP remains unexplored. AIM To investigate effect OGT on its role mediating O-GlcNAcylation p38. METHODS For vivo experiments, mice were divided into four groups: Control, model, model + short hairpin (sh) RNA-negative control, sh-OGT. Diabetes was induced streptozotocin, followed ligation lipopolysaccharide (LPS) administration to induce periodontitis. The impact assessed injecting sh-OGT lentivirus. Maxillary bone destruction evaluated using micro-computed tomography analysis tartrate-resistant acid phosphatase staining, while determined through quantitative real-time polymerase chain reaction (qPCR) immunohistochemistry. vitro RAW264.7 cells treated with LPS high glucose (HG) (25 mmol/L D-glucose) establish a cell DP. inhibited inhibitor (OSMI4) treatment knocked down transfection. M1/M2 analyzed qPCR, immunofluorescence, flow cytometry. Levels measured immunoprecipitation western blotting. RESULTS Our results demonstrated that led maxillary loss mice, associated elevated levels. Knockdown promoted shift from both mouse periodontal tissues HG-induced cells. Furthermore, HG enhanced interacted promote at residues A28, T241, T347, as well phosphorylation residue Y221. CONCLUSION Inhibition OGT-mediated deactivates pathway suppressing self-phosphorylation, thereby promoting mitigating These findings suggested modulating regulation may represent novel therapeutic strategy for treating

Язык: Английский

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Mitochondrial Dynamics and Metabolism in Macrophages for Cardiovascular Disease: a review

Phytomedicine, Год журнала: 2025, Номер 140, С. 156620 - 156620

Опубликована: Март 7, 2025

Язык: Английский

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Microbiota-induced lipid peroxidation impairs obeticholic acid-mediated antifibrotic effect towards nonalcoholic steatohepatitis in mice

Redox Biology, Год журнала: 2022, Номер 59, С. 102582 - 102582

Опубликована: Дек. 22, 2022

Obeticholic acid (OCA) has been examined to treat non-alcoholic steatohepatitis (NASH), but unsatisfactory antifibrotic effect and deficient responsive rate in recent phase III clinical trial. Using a prolonged western diet-feeding murine NASH model, we show that OCA-shaped gut microbiota induces lipid peroxidation impairs its anti-fibrotic effect. Mechanically, Bacteroides enriched by OCA deconjugates tauro-conjugated bile acids generate excessive chenodeoxycholic (CDCA), resulting liver ROS accumulation. We further elucidate reduces triglycerides containing polyunsaturated fatty (PUFA-TGs) levels, whereas elevates free PUFAs phosphatidylethanolamines PUFA (PUFA-PEs), which are susceptible be oxidized peroxides (notably arachidonic (ARA)-derived 12-HHTrE), inducing hepatocyte ferroptosis activating hepatic stellate cells (HSCs). Inhibiting with pentoxifylline (PTX) rescues of OCA, suggesting combination inhibitor could potential pharmacological approach NASH-fibrosis.

Язык: Английский

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Suppression of the SLC7A11/glutathione axis causes ferroptosis and apoptosis and alters the mitogen-activated protein kinase pathway in nasopharyngeal carcinoma

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 254, С. 127976 - 127976

Опубликована: Ноя. 10, 2023

Язык: Английский

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Biotin decorated celastrol-loaded ZIF-8 nano-drug delivery system targeted epithelial ovarian cancer therapy

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 167, С. 115573 - 115573

Опубликована: Сен. 26, 2023

Ovarian cancer (OC) stands as the second most prominent factor leading to cancer-related fatalities, characterized by a notably low five-year survival rate. The insidious onset of OC combined with its resistance chemotherapy poses significant challenges in terms treatment, emphasizing utmost importance developing innovative therapeutic agents. Despite remarkable anti-tumor efficacy, celastrol (CEL) faces regarding clinical utilization due restricted water solubility and notable side effects. In this study, was encapsulated into Zeolitic imidazolate framework-8(ZIF-8) nanoparticle grafted biotin-conjugated polyethylene glycol (CEL@ZIF-8@PEG-BIO). Comprehensive comparisons physicochemical properties anticancer activities CEL CEL@ZIF-8@PEG-BIO were conducted. Our findings revealed that exhibited favorable characteristics, including hydrodynamic diameters 234.5 nm, excellent solubility, high drug loading (31.60% ± 2.85), encapsulation efficiency (60.52% 2.79), minimal Furthermore, can release chemicals response an acidic micro-environment, which is more likely tumor micro-environment. vitro, studies showed CEL@ZIF-8@BIO inhibited cell proliferation, led mitochondrial membrane potential (MMP) decline, generated reactive oxygen species cells. Both vitro vivo experiments indicated enhanced activity against via up-regulated apoptosis-promoting biomarkers rendered apoptosis P38/JNK MAPK signaling pathway. conclusion, we have successfully developed novel delivery system (CEL@ZIF-8@PEG-BIO), resulting improvements both efficacy thereby providing valuable insights for future development.

Язык: Английский

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In vivo visualization of tumor-associated macrophages re-education by photoacoustic/fluorescence dual-modal imaging with a metal-organic frames-based caspase-1 nanoreporter

Journal of Colloid and Interface Science, Год журнала: 2023, Номер 659, С. 48 - 59

Опубликована: Дек. 21, 2023

Язык: Английский

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Cucurbitacin B modulates M2 macrophage differentiation and attenuates osteosarcoma progression via PI3K/AKT pathway

Phytotherapy Research, Год журнала: 2024, Номер 38(5), С. 2215 - 2233

Опубликована: Фев. 27, 2024

Abstract Osteosarcoma is a common malignant bone tumour characterised by an aggressive metastatic potential. The microenvironment, particularly the M2‐polarised macrophages, crucial for progression. Cucurbitacin B (CuB), triterpenoid derivative, recognised its anti‐inflammatory and antitumour properties. This study investigates CuB effect on M2 macrophage differentiation osteosarcoma progression, aiming to contribute new treatment strategies. In vitro, THP‐1 monocytes were stimulated with PMA, IL‐13 IL‐4 induce into macrophages. Additionally, influence of proliferation, migration invasion cells in context macrophages was scrutinised. Crucial signalling pathways, especially PI3K/AKT pathway, affected identified validated. vivo, model employed gauge effects weight, lung metastasis, angiogenesis, cell proliferation markers. results showed that inhibited differentiation, leading reduced cells. manifested inhibitory pathway during mouse models, markedly weight number metastases. It also expression angiogenesis markers tissues, decreased quantity their associated proteins. conclusion, impedes progression inhibiting via presenting potential therapeutic advancements treatment.

Язык: Английский

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The roles of gut microbiota metabolites in the occurrence and development of colorectal cancer: Multiple insights for potential clinical applications

Gastro Hep Advances, Год журнала: 2024, Номер 3(6), С. 855 - 870

Опубликована: Янв. 1, 2024

Язык: Английский

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Crosstalk between macrophages and immunometabolism and their potential roles in tissue repair and regeneration

Heliyon, Год журнала: 2024, Номер 10(18), С. e38018 - e38018

Опубликована: Сен. 1, 2024

Язык: Английский

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