DNA-Dependent Protein Kinase Catalytic Subunit Prevents Ferroptosis in Retinal Pigment Epithelial Cells DOI Creative Commons
Xueying Wang, Xi Wang, Zhenzhen Zhao

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(1), P. 50 - 50

Published: Jan. 22, 2025

Purpose: The purpose of this study was to investigate the activated core kinases involved in DNA damage responses (DDR) during ferroptosis retinal pigment epithelial (RPE) cells vitro and their regulatory effects on ferroptosis. Methods: Ferroptosis induced by erastin RPE (iRPE) derived from human umbilical cord mesenchymal stem (hUCMSCs), hUCMSCs, pluripotent cell-derived (iPSC-RPE) cells. CCK8 employed measure cell viability. Calcein/PI staining used detect ferroptotic γ-H2AX, 8-oxoG, phosphorylated DNA-dependent protein kinase catalytic subunit (DNA-PKcs) were determined through immunostaining. phosphorylation DNA-PKcs ERK1/2 Western blotting. Lipid peroxides detected BODIPY581/591-C11 staining. Results: iRPE exhibited a stronger ability resist compared hUCMSCs. cells, rapidly treatment erastin. In addition, inhibition promoted suggesting that prevents Meanwhile, inhibited only at early stage induction, whereas played protective role Furthermore, inducing its promoting also verified iPSC-RPE Conclusions: present elucidates key DDR is plays vitro, which will provide new research targets strategies for inhibiting

Language: Английский

Suppression of the SLC7A11/glutathione axis causes ferroptosis and apoptosis and alters the mitogen-activated protein kinase pathway in nasopharyngeal carcinoma DOI
Haihua Wang, Songqing Fan, Yuting Zhan

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127976 - 127976

Published: Nov. 10, 2023

Language: Английский

Citations

17
Application of calcium overload-based ion interference therapy in tumor treatment: strategies, outcomes, and prospects DOI Creative Commons
Shuangjiang Li,

Ruicheng Fan,

Y P Wang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 15, 2024

Low selectivity and tumor drug resistance are the main hinderances to conventional radiotherapy chemotherapy against tumor. Ion interference therapy is an innovative anti-tumor strategy that has been recently reported induce metabolic disorders inhibit proliferation of cells by reordering bioactive ions within cells. Calcium cation (Ca2+) indispensable for all physiological activities In particular, calcium overload, characterized abnormal intracellular Ca2+ accumulation, causes irreversible cell death. Consequently, overload-based ion potential overcome traditional treatment strategies holds promise clinical application. this review, we 1) Summed up current employed in therapy; 2) Described outcome death resulting from 3) Discussed its application synergistic with immunotherapy.

Language: Английский

Citations

8
Could Cytoplasmic Lipid Droplets be Linked to Inefficient Oxidative Phosphorylation in Cancer? DOI
Thomas N. Seyfried,

Nathan L. Ta,

Tomás Duraj

et al.

Current Tissue Microenvironment Reports, Journal Year: 2024, Volume and Issue: 5(4), P. 109 - 117

Published: May 17, 2024

Language: Английский

Citations

8
Microbiota-induced lipid peroxidation impairs obeticholic acid-mediated antifibrotic effect towards nonalcoholic steatohepatitis in mice DOI Creative Commons

Aoxiang Zhuge,

Shengjie Li,

Yin Yuan

et al.

Redox Biology, Journal Year: 2022, Volume and Issue: 59, P. 102582 - 102582

Published: Dec. 22, 2022

Obeticholic acid (OCA) has been examined to treat non-alcoholic steatohepatitis (NASH), but unsatisfactory antifibrotic effect and deficient responsive rate in recent phase III clinical trial. Using a prolonged western diet-feeding murine NASH model, we show that OCA-shaped gut microbiota induces lipid peroxidation impairs its anti-fibrotic effect. Mechanically, Bacteroides enriched by OCA deconjugates tauro-conjugated bile acids generate excessive chenodeoxycholic (CDCA), resulting liver ROS accumulation. We further elucidate reduces triglycerides containing polyunsaturated fatty (PUFA-TGs) levels, whereas elevates free PUFAs phosphatidylethanolamines PUFA (PUFA-PEs), which are susceptible be oxidized peroxides (notably arachidonic (ARA)-derived 12-HHTrE), inducing hepatocyte ferroptosis activating hepatic stellate cells (HSCs). Inhibiting with pentoxifylline (PTX) rescues of OCA, suggesting combination inhibitor could potential pharmacological approach NASH-fibrosis.

Language: Английский

Citations

28
Biotin decorated celastrol-loaded ZIF-8 nano-drug delivery system targeted epithelial ovarian cancer therapy DOI Open Access

Ruisi Zhou,

Yanting You, Zhiqiang Zha

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 167, P. 115573 - 115573

Published: Sept. 26, 2023

Ovarian cancer (OC) stands as the second most prominent factor leading to cancer-related fatalities, characterized by a notably low five-year survival rate. The insidious onset of OC combined with its resistance chemotherapy poses significant challenges in terms treatment, emphasizing utmost importance developing innovative therapeutic agents. Despite remarkable anti-tumor efficacy, celastrol (CEL) faces regarding clinical utilization due restricted water solubility and notable side effects. In this study, was encapsulated into Zeolitic imidazolate framework-8(ZIF-8) nanoparticle grafted biotin-conjugated polyethylene glycol (CEL@ZIF-8@PEG-BIO). Comprehensive comparisons physicochemical properties anticancer activities CEL CEL@ZIF-8@PEG-BIO were conducted. Our findings revealed that exhibited favorable characteristics, including hydrodynamic diameters 234.5 nm, excellent solubility, high drug loading (31.60% ± 2.85), encapsulation efficiency (60.52% 2.79), minimal Furthermore, can release chemicals response an acidic micro-environment, which is more likely tumor micro-environment. vitro, studies showed CEL@ZIF-8@BIO inhibited cell proliferation, led mitochondrial membrane potential (MMP) decline, generated reactive oxygen species cells. Both vitro vivo experiments indicated enhanced activity against via up-regulated apoptosis-promoting biomarkers rendered apoptosis P38/JNK MAPK signaling pathway. conclusion, we have successfully developed novel delivery system (CEL@ZIF-8@PEG-BIO), resulting improvements both efficacy thereby providing valuable insights for future development.

Language: Английский

Citations

15
Cucurbitacin B modulates M2 macrophage differentiation and attenuates osteosarcoma progression via PI3K/AKT pathway DOI
Hong Wu,

Tianjun Ma,

Mei He

et al.

Phytotherapy Research, Journal Year: 2024, Volume and Issue: 38(5), P. 2215 - 2233

Published: Feb. 27, 2024

Abstract Osteosarcoma is a common malignant bone tumour characterised by an aggressive metastatic potential. The microenvironment, particularly the M2‐polarised macrophages, crucial for progression. Cucurbitacin B (CuB), triterpenoid derivative, recognised its anti‐inflammatory and antitumour properties. This study investigates CuB effect on M2 macrophage differentiation osteosarcoma progression, aiming to contribute new treatment strategies. In vitro, THP‐1 monocytes were stimulated with PMA, IL‐13 IL‐4 induce into macrophages. Additionally, influence of proliferation, migration invasion cells in context macrophages was scrutinised. Crucial signalling pathways, especially PI3K/AKT pathway, affected identified validated. vivo, model employed gauge effects weight, lung metastasis, angiogenesis, cell proliferation markers. results showed that inhibited differentiation, leading reduced cells. manifested inhibitory pathway during mouse models, markedly weight number metastases. It also expression angiogenesis markers tissues, decreased quantity their associated proteins. conclusion, impedes progression inhibiting via presenting potential therapeutic advancements treatment.

Language: Английский

Citations

5
Crosstalk between macrophages and immunometabolism and their potential roles in tissue repair and regeneration DOI Creative Commons
Hongbo Ma, Limei Gao, Rong Chang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e38018 - e38018

Published: Sept. 1, 2024

Language: Английский

Citations

5
In vivo visualization of tumor-associated macrophages re-education by photoacoustic/fluorescence dual-modal imaging with a metal-organic frames-based caspase-1 nanoreporter DOI
Zhijin Fan, Xiaoxiao Jiang, T. Sun

et al.

Journal of Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 659, P. 48 - 59

Published: Dec. 21, 2023

Language: Английский

Citations

12
Advancements in the impact of human microbiota and probiotics on leukemia DOI Creative Commons
Yi Zhang, Xiaotong Zhao, Jingxian Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 3, 2024

The human gut microbiota is a complex ecosystem that plays crucial role in promoting the interaction between body and its environment. It has been increasingly recognized diverse physiological functions. Recent studies have shown close association development of certain tumors, including leukemia. Leukemia malignant clonal disease characterized by uncontrolled growth one or more types blood cells, which most common cancer children. imbalance linked to pathological mechanisms Probiotics, are beneficial microorganisms help maintain balance host microbiome, play regulating microbiota. Probiotics potential assist treatment leukemia improve clinical prognosis patients. This study reviews relationship microbiota, probiotics, progression based on current research. In addition, utilizing zebrafish models future might reveal specific their interactions, thereby providing new insights into conclusion, further investigation still needed fully understand accurate microbes

Language: Английский

Citations

4
Causal effects of circulating lipids and lipid-lowering drugs on the risk of epilepsy: a two-sample Mendelian randomization study DOI
Zhen Liang, Limei Zhao, Yu-Liang Lou

et al.

QJM, Journal Year: 2023, Volume and Issue: 116(6), P. 421 - 428

Published: March 25, 2023

Previous studies have reported inconsistent results on the association between circulating lipids and lipid-lowering drugs with risk of epilepsy.To assess whether genetically predicted are causally associated epilepsy outcome.We performed a two-sample Mendelian randomization (MR) analysis model to predict causal effects (apolipoprotein A [APOA], apolipoprotein B [APOB], cholesterol, high-density lipoprotein cholesterol [HDL-C], low-density [LDL-C], triglycerides) (HMG-CoA reductase [HMGCR] proprotein convertase subtilisin/kexin type 9 [PCSK9] inhibitors) epilepsy. Nine MR methods were conducted analyze final results. The inverse-variance weighted (IVW) method was used as primary outcome. other (simple mode, simple median, penalized Egger MR-Egger [bootstrap]) complement IVW. In addition, robustness assessed by leave-one-out analysis.The IVW demonstrated that there is no (APOA: odds ratio [OR], 0.958, 95% confidence interval (CI), 0.728-1.261, P = 0.760; APOB: OR, 1.092; CI, 0.979-1.219, 0.115; cholesterol: 1.210; 0.981-1.494, 0.077; HDL-C: 0.964; 0.767-1.212, 0.753; LDL-C: 1.100; 0.970-1.248, 0.137; A: 1.082; 0.849-1.379, 0.528; triglycerides: 1.126; 0.932-1.360, 0.221) (HMGCR inhibitors: 0.221; 0.006-8.408, 0.878; PCSK9 1.112; 0.215-5.761, 0.902) further sensitivity confirmed results.This study relationships

Language: Английский

Citations

10