Insect nuclear receptors: From orphans to ligands

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Herbicide prometryn adversely affects the development and reproduction of Tigriopus japonicus by disturbing the ecdysone signal pathway and chitin metabolic pathway

Aquatic Toxicology, Год журнала: 2022, Номер 254, С. 106378 - 106378

Опубликована: Дек. 19, 2022

Язык: Английский

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RNAi-Mediated Functional Analysis Reveals the Regulation of Oocyte Vitellogenesis by Ecdysone Signaling in Two Coleoptera Species

Biology, Год журнала: 2023, Номер 12(10), С. 1284 - 1284

Опубликована: Сен. 26, 2023

Coleoptera is the largest taxa of animals by far. The robust reproductive capacity one main reasons for such domination. Successful female reproduction partially relies on effective vitellogenesis. However, hormone regulation vitellogenesis remains to be explored. In present paper, in vitro culture Leptinotarsa decemlineata 1-day-old adult fat bodies 20E-contained median did not activate juvenile production and insulin-like peptide pathways, but significantly stimulated expression two LdVg genes, a cycloheximide-dependent pattern. vivo RNA interference (RNAi) either ecdysone receptor (LdEcR) or ultraspiracle (Ldusp) injection corresponding dsRNA into adults inhibited oocyte development, dramatically repressed transcription genes LdVgR ovaries; application JH LdEcR Ldusp RNAi L. females restore rescued decreased mRNA levels over-compensated levels. same experiments were performed another species, Henosepilachna vigintioctopunctata. Little yolk substances seen misshapen oocytes HvEcR Hvusp ovaries, contrast larger amounts granules normal oocytes. Correspondingly, transcript HvVg ovaries samples. Our results here show that 20E signaling indispensable activation developing beetle species.

Язык: Английский

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Effect of silencing the E74B gene on the development and metamorphosis of Helicoverpa armigera

Pest Management Science, Год журнала: 2023, Номер 80(3), С. 1435 - 1445

Опубликована: Ноя. 8, 2023

Abstract BACKGROUND The growth and development transition of insects are mainly mediated by ecdysone. As one the ecdysone‐induced transcription factors, E74 is involved in many physiological processes insect development. However, its function Helicoverpa armigera remains unclear. RESULTS In this study, E74B , a subtype E74, was identified for first time H. armigera. Bioinformatics analysis showed that shared highest homology with Bombyx mori, which belongs to E26 transformation‐specific (ETS) superfamily. expression profile level HaE74B increased late stages fourth sixth instars compared early stages; it also high pupa midgut. Moreover, we investigated through RNA interference 20E rescue experiments. results silencing affected molting larvae, resulting death more than 60% larvae. addition, seriously metamorphosis reduced pupae rate, eclosion rate pupae, fecundity. Application partially restored defects molting, . CONCLUSION Taken together, these demonstrated plays critical role growth, development, armigera, serves as molecular target sets out theoretical foundation RNAi‐mediated control key pest. © 2023 Society Chemical Industry.

Язык: Английский

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Research Progress on the Regulation of Autophagy and Apoptosis in Insects by Sterol Hormone 20-Hydroxyecdysone

Insects, Год журнала: 2023, Номер 14(11), С. 871 - 871

Опубликована: Ноя. 12, 2023

20E (20-Hydroxyecdysone) is a central steroid hormone that orchestrates developmental changes and metamorphosis in arthropods. While its molecular mechanisms have been recognized for some time, detailed elucidation has primarily emerged the past decade. PCD (Programmed cell death), including apoptosis, necrosis, efferocytosis, pyroptosis, ferroptosis, autophagy, plays crucial role regulated elimination, which vital cells’ development tissue homeostasis. This review summarizes recent findings on signaling autophagy apoptosis insects, Drosophila melanogaster, Bombyx mori, Helicoverpa armigera, other species. Firstly, we comprehensively explore biosynthesis of sterol subsequent signal transduction various Then, focus involvement regulating elucidating roles both contexts bacterial infection scenarios. Furthermore, our discussion unfolds as panoramic exposition, where delve into fundamental questions with findings, anchoring them within grander scheme study insects. Deepening understanding 20E-autophagy/apoptosis axis not only underscores intricate tapestry endocrine networks, but also offers fresh perspectives adaptive evolved face environmental challenges.

Язык: Английский

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Cell cycle variants duringDrosophilaaccessory gland development

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2019, Номер unknown

Опубликована: Июль 30, 2019

Abstract The Drosophila melanogaster accessory gland is a functional analog of the mammalian prostate containing two secretory epithelial cell types, termed main and secondary cells. This tissue responsible for making secreting seminal fluid proteins other molecules that contribute to successful reproduction. cells this are bi-nucleate polyploid, due variant cycles include endomitosis endocycling during metamorphosis. Here we provide evidence additional cycle variants in tissue. We show connected by ring canals form after penultimate mitosis describe an post-eclosion endocycle required maturation dependent on juvenile hormone signaling. present undergo unique reprogramming throughout organ development results step-wise truncations culminating octoploid nuclei with under-replicated heterochromatin mature gland. propose as model study developmental hormonal temporal control terminally differentiating tissues.

Язык: Английский

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Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster

BMC Biology, Год журнала: 2024, Номер 22(1)

Опубликована: Май 13, 2024

Abstract Background Juvenile hormones (JH) play crucial role in regulating development and reproduction insects. The most common form of JH is III, derived from MF through epoxidation by CYP15 enzymes. However, the higher dipterans, such as fruitfly, Drosophila melanogaster , a bis-epoxide JHB3, accounted detected. Moreover, these dipterans have lost gene their genomes. As result, identity P450 epoxidase biosynthesis pathway remains unknown. Results In this study, we show that Cyp6g2 serves major responsible for JHB3 III D. . predominantly expressed corpus allatum (CA), concurring with expression pattern jhamt another well-studied last steps biosynthesis. Mutation leads to severe disruptions larval-pupal metamorphosis exhibits reproductive deficiencies, exceeding those seen mutants. Notably, −/− ::jhamt 2 double mutants all died at pupal stage but could be rescued topical application analogs. titer analyses revealed both mutant lacking while overexpression or caused significant increase titer. Conclusions These findings collectively established laid groundwork further understanding pave way developing specific inhibitors insect growth regulators insecticides.

Язык: Английский

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Functional analysis of nuclear receptor genes in molting and metamorphosis of the cigarette beetle, Lasioderma serricorne

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 270, С. 132459 - 132459

Опубликована: Май 18, 2024

Язык: Английский

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Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone

Опубликована: Окт. 24, 2024

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

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First micro‐transcriptome of the third instar larvae of Anastrepha obliqua and its association with polyphagia

Journal of Applied Entomology, Год журнала: 2022, Номер 146(6), С. 700 - 709

Опубликована: Март 31, 2022

Abstract Phytophagous insects are organisms that use a part of plant as food resource. Within this group, we focused on the pest insect Anastrepha obliqua , fly feeds fruits plants different species and causes economic losses American continent. The ability larvae to feed various (polyphagia) has been studied from perspectives but never at level microRNAs. These interfering RNAs can regulate gene expression in tissues organs an organism. objective work was obtain first micro‐transcriptome determine its levels possible target mRNAs when third instar A . three species: Spondias purpurea Mangifera indica Averrhoa star fruit total 116 microRNAs were identified study, which 37 completely new. Fifty‐four expressed all larvae, regardless fruit, while 44 detected fed specific fruit. Twenty‐one showed differential expression, annotation targets these have important genes play roles development, feeding detoxification targets. construction identification for provide new information understanding mechanisms control dipteran.

Язык: Английский

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