Cellular and Molecular Life Sciences, Год журнала: 2023, Номер 80(8)
Опубликована: Июль 22, 2023
Язык: Английский
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3322 - 3322
Опубликована: Март 15, 2024
The highly conserved Notch signaling pathway affects embryonic development, neurogenesis, homeostasis, tissue repair, immunity, and numerous other essential processes. Although previous studies have demonstrated the location function of core components in various animal phyla, a more comprehensive summary lower organisms is still required. In this review, we objectively summarize molecular features constituents, their current expression profiles, functions invertebrates, with emphasis on effects neurogenesis regeneration. We also analyze evolution facets hope that contents review will be useful to interested researchers.
Язык: Английский
Процитировано
9
Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 173, С. 116364 - 116364
Опубликована: Март 5, 2024
This study was to investigate the potential mechanisms of treatment with metformin (Met) combined kaempferol (Kae) against postmenopausal osteoporosis. Experiments were conducted in both ovariectomy (OVX)-induced osteoporosis rats and vitro using RAW264.7 cells, MC3T3-E1 HUVECs. Results demonstrated therapeutic effect Met Kae on In vivo, alone combination treatments enhanced tibial trabecular microstructure, bone mineral density (BMD), mechanical properties OVX without causing hepatotoxicity nephrotoxicity. It also reduced resorption markers (CTX-1 TRAP) increased formation marker (PINP) level serum rats. The expression TRAP reduced, while Runx2 ALP tissue Furthermore, promoted angiogenesis-related CD31 VEGF vitro, MC3T3-E1s cells treated showed higher ALP, Runx2, VEGF. Interestingly, did not directly promote HUVECs migration angiogenesis, but osteoblast-mediated angiogenesis by upregulating levels. Additionally, secretion MC3T3-E1, activated Notch intracelluar pathway HES1 HEY1 Meantime, their stimulation inhibited DAPT, a signaling inhibitor. Overall, this demonstrates positive effects osteoporotic promoting osteogenesis-angiogenesis coupling, suggesting application
Язык: Английский
Процитировано
7
The Science of The Total Environment, Год журнала: 2024, Номер 924, С. 171653 - 171653
Опубликована: Март 12, 2024
Язык: Английский
Процитировано
7
Molecular and Cellular Biochemistry, Год журнала: 2024, Номер unknown
Опубликована: Апрель 3, 2024
Язык: Английский
Процитировано
6
Pharmacological Research, Год журнала: 2023, Номер 194, С. 106819 - 106819
Опубликована: Июнь 13, 2023
Lung cancer is the main reason for cancer-associated death globally, and lung adenocarcinoma (LUAD) most prevalent subtype of cancer. Recently, AGRN considered playing an vital role in development some cancers. However, regulatory effects mechanisms LUAD remain elusive. In this study, we clarified significant upregulation expression by single-cell RNA sequencing combined with immunohistochemistry. Besides, confirmed that patients high are more susceptible to lymph node metastases have a worse prognosis retrospective study 120 patients. Next, demonstrated directly interact NOTCH1, which results release intracellular structural domain NOTCH1 subsequent activation NOTCH pathway. Moreover, also found promotes proliferation, migration, invasion, EMT tumorigenesis cells vitro vivo, these reversed blocking Furthermore, prepared several antibodies targeting AGRN, clarify Anti-AGRN antibody treatment could significantly inhibit proliferation promote apoptosis tumor cells. Our highlights important mechanism progression, suggests therapeutic potential LUAD. We provide theoretical experimental evidence further monoclonal AGRN.
Язык: Английский
Процитировано
14
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 30, 2025
Regeneration plays a key role in energy recycling and homeostasis maintenance. Planarians, as ideal model animals for studying regeneration, stem cell proliferation, apoptosis, have the strong regenerative abilities. Considerable evidence suggests that ubiquitin an important maintaining regulating but function of Ubiquitin specific proteases 7 (Usp7) on regeneration planarians remains elusive. We identified evolutionarily conserved gene, Usp7, utilized RNA interference (RNAi), Quantitative real-time PCR (qRT-PCR), Whole-mount immunofluorescence, Tunnel, situ hybridization (WISH), western blotting to detect Usp7 during planarian regeneration. In this study, we found trunk fragments RNAi worms could not regenerate missing tails; meanwhile, level proliferation was decreased, while apoptosis increased. Furthermore, expression Islet inhibited The signal wnt1/P-1 exhibited dot-like pattern at posterior regenerating after 1 day (R d). However, concentrated dramatically declined 3 days d) disappeared addition, activating Wnt pathway partially rescued defects induced by inhibition Usp7. Collectively, is necessary tissue tail blastema formation It also promote polarity reconstruction via Islet/Wnt1 axis.
Язык: Английский
Процитировано
0
Aquatic Toxicology, Год журнала: 2025, Номер 282, С. 107310 - 107310
Опубликована: Март 4, 2025
Язык: Английский
Процитировано
0
Advanced Pharmaceutical Bulletin, Год журнала: 2024, Номер 14(2), С. 302 - 313
Опубликована: Март 10, 2024
Cancer is one of the main causes mortality worldwide. cells are characterized by unregulated cellular processes, including proliferation, progression, and angiogenesis. The occurrence these processes due to dysregulation various signaling pathways such as NF-κB (nuclear factor-κB), Wnt/beta-catenin, Notch MAPK (mitogen-activated protein kinases). cause progression types malignant tumors. Among phytochemicals for cancer therapy, several have attracted great interest, curcumin, genistein, quercetin, silibinin, resveratrol, cucurbitacin glycyrrhizin. Given molecular heterogeneity within tumors high toxicity side effects synthetic chemotherapeutics, natural products with pleiotropic that simultaneously target numerous appear be ideal substitutes therapy. With this in mind, we take a look at current status, impact potential known compounds golden on key tumors, focusing pathway. This review may useful discovering new targets safe efficient therapy chemotherapeutics.
Язык: Английский
Процитировано
4
Animals, Год журнала: 2025, Номер 15(2), С. 200 - 200
Опубликована: Янв. 13, 2025
Asthma has been extensively studied in humans and animals, but the molecular mechanisms underlying asthma Meishan pigs, a breed with distinct genetic physiological characteristics, remain elusive. Understanding these could provide insights into veterinary medicine human research. We investigated pathogenesis pigs through transcriptomic metabolomic analyses of blood samples taken during autumn winter. is related to inflammation, mitochondrial oxidative phosphorylation, tricarboxylic acid (TCA) cycle disorders. Related genes include CXCL10, CCL8, CCL22, CCL21, OLR1, ACKR1, while metabolites succinic acid, riboflavin-5-phosphate, fumaric acid. Transcriptomic sequencing was performed on panting normal differentially expressed underwent functional enrichment screening. Metabolomic analysis revealed differential pathways between groups. Combined indicated that lung inflammation influenced by genetic, allergenic, environmental factors disrupting phosphorylation mitochondria, affecting TCA cycle. Mitochondrial reactive oxygen species, glutathione S-transferases, arginase 1 RORC immune regulation, Notch pathway, YPEL4 cell proliferation, MARCKS airway mucus secretion play roles pathogenesis. This study highlights many cytokines signaling contribute asthma. Further studies are needed elucidate their complex interactions.
Язык: Английский
Процитировано
0
Molecular Neurobiology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 30, 2025
Stress urinary incontinence (SUI) currently lacks effective treatment options, and the restoration of neurological function remains a major challenge, with unmet clinical needs. Research has indicated that adipose-derived stem cells (ADSCs) can be induced to differentiate into neural-induced (NI-ADSCs) under specific inductive conditions, exhibiting excellent neuroregenerative capabilities. ADSCs were obtained from female SD rats NI-ADSCs. In vitro, NI-ADSCs co-cultured Schwann (SCs) investigate their effects on SC proliferation repair phenotype transition further explore its underlying mechanism. vivo, rat model SUI was established using bilateral pudendal nerve transection method. injected urethral sphincter evaluate urodynamics, muscle angiogenesis, neural in rats, while also exploring mechanisms repair. This study used EGF, FGF, B27 induce expressing induction markers (MAP, Nestin, PAX6). vitro experiments found no significant difference L6 RSC96 between (p > 0.05). However, when NI-ADSCs, SCs showed upregulated expression repair-related phenotypic (BDNF, GDNF, GFAP). this transformation process, Notch-related pathway proteins (Notch1, NICD, Hes1) increased, use DAPT (a Notch inhibitor) could suppress transformation. revealed intraurethral injection significantly promoted marker (S100β) demyelination (GFAP) urodynamic recovery inhibited effect. summary, our demonstrates promote regeneration by promoting maintaining SCs. The mechanism may related activation signaling pathway.
Язык: Английский
Процитировано
0