Water Research, Год журнала: 2022, Номер 219, С. 118535 - 118535
Опубликована: Май 3, 2022
Язык: Английский
Water Research, Год журнала: 2022, Номер 219, С. 118535 - 118535
Опубликована: Май 3, 2022
Язык: Английский
The Science of The Total Environment, Год журнала: 2022, Номер 824, С. 153687 - 153687
Опубликована: Фев. 5, 2022
Язык: Английский
Процитировано
88ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 1992 - 2003
Опубликована: Май 26, 2022
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two sewersheds different scales: a University campus treatment plant. Wastewater data were combined with complementary COVID-19 case evaluate the efficiency surveillance forecasting new cases and, larger scale, hospitalizations. Results show normalization PMMoV B2M resulted correlations using volcano second generation (V2G)-qPCR chemistry (rs = 0.69 without normalization, rs 0.73 normalization). Mixed results obtained samples collected at scale. Overall benefits measures depend qPCR improves smaller sewershed We recommend further studies efficacy additional targets.
Язык: Английский
Процитировано
84The Science of The Total Environment, Год журнала: 2022, Номер 821, С. 153291 - 153291
Опубликована: Янв. 25, 2022
As COVID-19 continues to spread globally, monitoring the disease at different scales is critical support public health decision making. Surveillance for SARS-CoV-2 RNA in wastewater can supplement surveillance based on diagnostic testing. In this paper, we report results of wastewater-based Emory University campus that included routine sampling sewage from a hospital building, an isolation/quarantine and 21 student residence halls between July 13th, 2020 March 14th, 2021. We examined sensitivity detecting cases building level relation Ct values RT-qPCR samples number patients residing building. Our show weekly using Moore swab was not sensitive enough (6 63 times) reliably detect one or two sporadic The over time same location reflected temporal trend (Pearson's r < −0.8), but there too much uncertainty directly estimate values. After students returned spring 2021 semester, detected most hall sites weeks before surged campus. This finding suggests be used provide early warning outbreaks institutions.
Язык: Английский
Процитировано
79Water Research, Год журнала: 2022, Номер 218, С. 118451 - 118451
Опубликована: Апрель 13, 2022
Язык: Английский
Процитировано
79Clinical Microbiology Reviews, Год журнала: 2023, Номер 37(1)
Опубликована: Дек. 14, 2023
SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.
Язык: Английский
Процитировано
68Water Research, Год журнала: 2023, Номер 231, С. 119648 - 119648
Опубликована: Янв. 20, 2023
Язык: Английский
Процитировано
50Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 12
Опубликована: Янв. 5, 2023
The coronavirus disease 2019 (COVID-19) pandemic has prompted a lot of questions globally regarding the range information about virus’s possible routes transmission, diagnostics, and therapeutic tools. Worldwide studies have pointed out importance monitoring early surveillance techniques based on identification viral RNA in wastewater. These indicated presence severe acute respiratory syndrome 2 (SARS-CoV-2) human feces, which is shed via excreta including mucus, saliva, sputum. Subsequently, they get dumped into wastewater, their wastewater provides possibility using it as tool to help prevent eradicate virus. Its still done many regions worldwide serves an “warning signal”; however, limitations also been identified.
Язык: Английский
Процитировано
45Water Research X, Год журнала: 2021, Номер 12, С. 100111 - 100111
Опубликована: Июль 31, 2021
Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA can be integrated with COVID-19 case data to inform timely pandemic response. However, more research is needed apply and develop systematic methods interpret the true SARS-CoV-2 signal from noise introduced in wastewater samples (e.g., sewer conditions, sampling extraction methods, etc.). In this study, raw was collected weekly five sewersheds one residential facility. The concentrations of were compared geocoded clinical testing data. reliably detected (95% positivity) frozen when reported daily new cases 2.4 or per 100,000 people. To adjust variation sample fecal content, four normalization biomarkers evaluated: crAssphage, pepper mild mottle virus, Bacteroides ribosomal (rRNA), human 18S rRNA. Of these, crAssphage displayed least spatial temporal variability. Both unnormalized normalized had positive significant correlation (Kendall's Tau-b (τ)=0.43 0.38, respectively), but no biomarker strengthened Locational dependencies date associated impacted lead time trends, observed collection (versus result date) used both This study supports that trends reflect disease occurrence presents tools could applied make interpretable comparable across studies.
Язык: Английский
Процитировано
106Journal of Virological Methods, Год журнала: 2021, Номер 297, С. 114230 - 114230
Опубликована: Июль 9, 2021
Язык: Английский
Процитировано
84Microbiology Spectrum, Год журнала: 2021, Номер 9(2)
Опубликована: Окт. 7, 2021
A wastewater surveillance program targeting a university residence hall was implemented during the spring semester 2021 as proactive measure to avoid an outbreak of COVID-19 on campus. Over period 7 weeks from early February through late March 2021, originating collected grab samples 3 times per week. During this time, there no detection SARS-CoV-2 by reverse transcriptase quantitative PCR (RT-qPCR) in stream. Aiming obtain sample more representative community, decision made use passive samplers beginning onwards. Adopting Moore swab approach, detected just 2 days after were deployed. These also tested positive for B.1.1.7 (Alpha) variant concern (VOC) using RT-qPCR. The result triggered public health case-finding response, including mobile testing unit deployed following day, with nearly 200 students and staff, which identified two laboratory-confirmed cases Alpha COVID-19. individuals relocated separate quarantine facility, averting Aggregating clinical data, campus has yielded first estimates fecal shedding rates VOC nonclinical setting. IMPORTANCE Among adopters monitoring have been colleges universities throughout North America, many whom are approach monitor congregate living facilities evidence infection integral component screening programs. Yet, while numerous examples where among community members, few response that may averted actual outbreak. This report details wastewater-testing when mounting globally over emergence variants concern, reported be transmissible than wild-type Wuhan strain. In communication, we present clear example how resulted actionable responses administration health,
Язык: Английский
Процитировано
81