Detection of influenza virus in urban wastewater during the season 2022/2023 in Sicily, Italy DOI Creative Commons
Carmelo Massimo Maida, Walter Mazzucco,

Walter Priano

и другие.

Frontiers in Public Health, Год журнала: 2024, Номер 12

Опубликована: Июль 23, 2024

Introduction Seasonal influenza generally represents an underestimated public health problem with significant socioeconomic implications. Monitoring and detecting epidemics are important tasks that require integrated strategies. Wastewater-based epidemiology (WBE) is emerging field uses wastewater data to monitor the spread of disease assess a community. It can represent integrative surveillance tool for better understanding prevention strategies in health. Methods We conducted study detected presence Influenza virus RNA using wastewater-based approach. Samples were collected from five treatment plants different municipalities, serving cumulative population 555,673 Sicilian inhabitants Italy. used RT-qPCR test compare combined weekly average A B viral samples incidence Influenza-like illness (ILI) obtained Italian national system. also compared number positive swabs loads wastewater. Our investigated 189 samples. Results Cumulative ILI cases substantially overlapped load trends similar rise population. Therefore, confirmed co-circulation viruses during season 2022/2023, trend reported clinically cases. Conclusion does not replace traditional epidemiological methods, such as laboratory testing infected individuals. However, it be valuable complement obtaining additional information on preventing its spread.

Язык: Английский

Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings DOI Creative Commons

Alessia Mirabile,

Giuseppe Sangiorgio, Paolo Giuseppe Bonacci

и другие.

Diagnostics, Год журнала: 2024, Номер 14(15), С. 1598 - 1598

Опубликована: Июль 25, 2024

Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision sensitivity nucleic acid detection quantification. This review highlights the core principles transformative potential of dPCR, particularly infectious disease diagnostics environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification target acids through advanced partitioning techniques. The addresses significant impact sepsis diagnosis management, showcasing superior specificity early pathogen identification drug-resistant genes. Despite advantages, challenges such optimization experimental conditions, standardization data analysis workflows, high costs are discussed. Furthermore, we compare various commercially available platforms, detailing their features applications clinical research settings. Additionally, explores dPCR's role water microbiology, wastewater surveillance monitoring waterborne pathogens, underscoring importance public health protection. In conclusion, future prospects including methodological optimization, integration with innovative technologies, expansion into new sectors like metagenomics, explored.

Язык: Английский

Процитировано

9
Human viral pathogens in the wastewater-source water-drinking water continuum: Evidence, health risks, and lessons for future outbreaks in low-income settings DOI
Willis Gwenzi, Bashir Adelodun, Pankaj Kumar

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 918, С. 170214 - 170214

Опубликована: Янв. 24, 2024

Язык: Английский

Процитировано

7
Wastewater-based epidemiology for preventing outbreaks and epidemics in Latin America – Lessons from the past and a look to the future DOI Creative Commons
Tatiana Prado, Gloria Rey-Benito, Marize Pereira Miagostovich

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 865, С. 161210 - 161210

Опубликована: Дек. 27, 2022

Wastewater-based epidemiology (WBE) is an approach with the potential to complement clinical surveillance systems. Using WBE, it possible carry out early warning of a outbreak, monitor spatial and temporal trends infectious diseases, produce real-time results generate representative epidemiological information in territory, especially areas social vulnerability. Despite historical uses this approach, particularly Global Polio Eradication Initiative, for other pathogens, was during COVID-19 pandemic that occurred exponential increase environmental programs SARS-CoV-2 wastewater, many experiences developments field public health using data decision making prioritizing actions control pandemic. In Latin America, WBE applied heterogeneous contexts emphasis on populations present socio-environmental inequalities, condition shared by all American countries. This manuscript addresses concepts applications actions, as well different countries, discusses model implement system at local or national level. We emphasize need sentinel countries want detect entry spread new pathogens outbreaks epidemics agents their territories

Язык: Английский

Процитировано

24
Wastewater-based epidemiology for COVID-19 surveillance and beyond: A survey DOI Creative Commons
Chen Chen,

Yunfan Wang,

Gursharn Kaur

и другие.

Epidemics, Год журнала: 2024, Номер 49, С. 100793 - 100793

Опубликована: Сен. 26, 2024

Язык: Английский

Процитировано

6
Wastewater, waste, and water-based epidemiology (WWW-BE): A novel hypothesis and decision-support tool to unravel COVID-19 in low-income settings? DOI Open Access
Willis Gwenzi

The Science of The Total Environment, Год журнала: 2021, Номер 806, С. 150680 - 150680

Опубликована: Сен. 30, 2021

Язык: Английский

Процитировано

32
The feasibility of SARS-CoV-2 surveillance using wastewater and environmental sampling in Indonesia DOI Creative Commons
Indah Kartika Murni, Vicka Oktaria, Amanda Handley

и другие.

PLoS ONE, Год журнала: 2022, Номер 17(10), С. e0274793 - e0274793

Опубликована: Окт. 14, 2022

Wastewater-based epidemiology (WBE) surveillance as an early warning system (EWS) for monitoring community transmission of SARS-CoV-2 in low- and middle-income country (LMIC) settings, where diagnostic testing capacity is limited, needs further exploration. We explored the feasibility to conduct a WBE Indonesia, one global epicenters COVID-19 pandemic middle 2021, with fourth largest population world sewer non-sewered sewage systems are implemented. The resource collect samples on weekly or fortnightly basis grab and/or passive sampling methods, well qualitative quantitative identification ribonucleic acid (RNA) using real-time RT-PCR (RT-qPCR) environmental were explored.We initiated routine wastewater at three predetermined districts Special Region Yogyakarta Province. Water collected from central treatment plants (WWTPs), including manholes flowing WWTP, additional soil near source tracking (NST) locations (i.e., public spaces people congregate).We began collecting Delta wave Indonesia July 2021. From 10-week period, 54% (296/544) positive RNA. sample positivity rate decreased proportion reported incidence clinical cases community. highest 77% week 1, was obtained 2021 25% 10 by end September 2021.A feasible monitor burden infections. Future studies potential EWS signaling outbreaks transmissions this setting required.

Язык: Английский

Процитировано

22
Quantification of SARS-CoV-2 in wastewater samples from hospitals treating COVID-19 patients during the first wave of the pandemic in Brazil DOI
Juliana Calábria de Araújo, Camila Leite Madeira, Thiago Bressani Ribeiro

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 860, С. 160498 - 160498

Опубликована: Ноя. 25, 2022

Язык: Английский

Процитировано

21
Model training periods impact estimation of COVID-19 incidence from wastewater viral loads DOI Creative Commons
Maria L. Daza–Torres, J. Cricelio Montesinos-López, Minji Kim

и другие.

The Science of The Total Environment, Год журнала: 2022, Номер 858, С. 159680 - 159680

Опубликована: Окт. 26, 2022

Wastewater-based epidemiology (WBE) has been deployed broadly as an early warning tool for emerging COVID-19 outbreaks. WBE can inform targeted interventions and identify communities with high transmission, enabling quick effective responses. As the wastewater (WW) becomes increasingly important indicator more robust methods metrics are needed to guide public health decision-making. This research aimed develop implement a mathematical framework infer incident cases of from SARS-CoV-2 levels measured in WW. We propose classification scheme assess adequacy model training periods based on clinical testing rates sensitivity predictions periods. A period is classified adequate when rate change greater than cases. present Bayesian deconvolution linear regression estimate WW data. The reproductive number estimated reconstructed using proposed modeling was applied three Northern California served by distinct treatment plants. results showed that essential provide accurate projections incidence.

Язык: Английский

Процитировано

20
Wastewater-Based Surveillance of Antibiotic Resistance Genes Associated with Tuberculosis Treatment Regimen in KwaZulu Natal, South Africa DOI Creative Commons
Hlengiwe N. Mtetwa, Isaac Dennis Amoah, Sheena Kumari

и другие.

Antibiotics, Год журнала: 2021, Номер 10(11), С. 1362 - 1362

Опубликована: Ноя. 8, 2021

Essential components of public health include strengthening the surveillance infectious diseases and developing early detection prevention policies. This is particularly important for drug-resistant tuberculosis (DR-TB), which can be explored by using wastewater-based surveillance. study aimed to use molecular techniques determine occurrence concentration antibiotic-resistance genes (ARGs) associated with (TB) resistance in untreated treated wastewater. Raw/untreated (post-chlorination) wastewater samples were taken from three treatment plants (WWTPs) South Africa. The ARGs selected target drugs used first- second-line TB treatment. Both conventional polymerase chain reaction (PCR) more advanced droplet digital PCR (ddPCR) evaluated as strategies distribution ARGs. most abundant ARG was rrs gene, aminoglycosides, specifically streptomycin, median ranges 4.69-5.19 log copies/mL. In contrast, pncA drug pyrazinamide, least detected (1.59 2.27 copies/mL). Resistance bedaquiline detected, a significant finding because this new introduced Africa multi-drug resistant TB. study, therefore, establishes potential monitoring antibiotic communities.

Язык: Английский

Процитировано

24
Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain DOI Creative Commons
Noelia Trigo‐Tasende, Juán A. Vallejo, Soraya Rumbo‐Feal

и другие.

Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(32), С. 79315 - 79334

Опубликована: Июнь 7, 2023

Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect SARS-CoV-2 mutations wastewater, respectively. In addition, own statistical models applied estimate real number infected people frequency each emerging variant circulating community, which considerable improved strategy. Our analysis detected 6 waves with concentrations between 10 3 RNA copies/L. system able anticipate outbreaks during 8–36 days advance respect clinical reports and, emergence new variants such Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529 BA.2) 42, 30, 27 days, respectively, before did. Data generated here helped local authorities managers give faster more efficient response situation, also allowed important industrial companies adapt their production situation. wastewater-based developed our metropolitan area (Spain) served powerful combining monitoring over time.

Язык: Английский

Процитировано

10