Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain DOI Creative Commons
Noelia Trigo‐Tasende, Juán A. Vallejo, Soraya Rumbo‐Feal

et al.

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(32), P. 79315 - 79334

Published: June 7, 2023

Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect SARS-CoV-2 mutations wastewater, respectively. In addition, own statistical models applied estimate real number infected people frequency each emerging variant circulating community, which considerable improved strategy. Our analysis detected 6 waves with concentrations between 10 3 RNA copies/L. system able anticipate outbreaks during 8–36 days advance respect clinical reports and, emergence new variants such Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529 BA.2) 42, 30, 27 days, respectively, before did. Data generated here helped local authorities managers give faster more efficient response situation, also allowed important industrial companies adapt their production situation. wastewater-based developed our metropolitan area (Spain) served powerful combining monitoring over time.

Language: Английский

Advancing Pathogen Identification: The Role of Digital PCR in Enhancing Diagnostic Power in Different Settings DOI Creative Commons

Alessia Mirabile,

Giuseppe Sangiorgio, Paolo Giuseppe Bonacci

et al.

Diagnostics, Journal Year: 2024, Volume and Issue: 14(15), P. 1598 - 1598

Published: July 25, 2024

Digital polymerase chain reaction (dPCR) has emerged as a groundbreaking technology in molecular biology and diagnostics, offering exceptional precision sensitivity nucleic acid detection quantification. This review highlights the core principles transformative potential of dPCR, particularly infectious disease diagnostics environmental surveillance. Emphasizing its evolution from traditional PCR, dPCR provides accurate absolute quantification target acids through advanced partitioning techniques. The addresses significant impact sepsis diagnosis management, showcasing superior specificity early pathogen identification drug-resistant genes. Despite advantages, challenges such optimization experimental conditions, standardization data analysis workflows, high costs are discussed. Furthermore, we compare various commercially available platforms, detailing their features applications clinical research settings. Additionally, explores dPCR's role water microbiology, wastewater surveillance monitoring waterborne pathogens, underscoring importance public health protection. In conclusion, future prospects including methodological optimization, integration with innovative technologies, expansion into new sectors like metagenomics, explored.

Language: Английский

Citations

8
Human viral pathogens in the wastewater-source water-drinking water continuum: Evidence, health risks, and lessons for future outbreaks in low-income settings DOI
Willis Gwenzi, Bashir Adelodun, Pankaj Kumar

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170214 - 170214

Published: Jan. 24, 2024

Language: Английский

Citations

7
Wastewater-based epidemiology for preventing outbreaks and epidemics in Latin America – Lessons from the past and a look to the future DOI Creative Commons
Tatiana Prado, Gloria Rey-Benito, Marize Pereira Miagostovich

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 865, P. 161210 - 161210

Published: Dec. 27, 2022

Wastewater-based epidemiology (WBE) is an approach with the potential to complement clinical surveillance systems. Using WBE, it possible carry out early warning of a outbreak, monitor spatial and temporal trends infectious diseases, produce real-time results generate representative epidemiological information in territory, especially areas social vulnerability. Despite historical uses this approach, particularly Global Polio Eradication Initiative, for other pathogens, was during COVID-19 pandemic that occurred exponential increase environmental programs SARS-CoV-2 wastewater, many experiences developments field public health using data decision making prioritizing actions control pandemic. In Latin America, WBE applied heterogeneous contexts emphasis on populations present socio-environmental inequalities, condition shared by all American countries. This manuscript addresses concepts applications actions, as well different countries, discusses model implement system at local or national level. We emphasize need sentinel countries want detect entry spread new pathogens outbreaks epidemics agents their territories

Language: Английский

Citations

23
Wastewater-based epidemiology for COVID-19 surveillance and beyond: A survey DOI Creative Commons
Chen Chen,

Yunfan Wang,

Gursharn Kaur

et al.

Epidemics, Journal Year: 2024, Volume and Issue: 49, P. 100793 - 100793

Published: Sept. 26, 2024

Language: Английский

Citations

5
Wastewater, waste, and water-based epidemiology (WWW-BE): A novel hypothesis and decision-support tool to unravel COVID-19 in low-income settings? DOI Open Access
Willis Gwenzi

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 806, P. 150680 - 150680

Published: Sept. 30, 2021

Language: Английский

Citations

32
The feasibility of SARS-CoV-2 surveillance using wastewater and environmental sampling in Indonesia DOI Creative Commons
Indah Kartika Murni, Vicka Oktaria, Amanda Handley

et al.

PLoS ONE, Journal Year: 2022, Volume and Issue: 17(10), P. e0274793 - e0274793

Published: Oct. 14, 2022

Wastewater-based epidemiology (WBE) surveillance as an early warning system (EWS) for monitoring community transmission of SARS-CoV-2 in low- and middle-income country (LMIC) settings, where diagnostic testing capacity is limited, needs further exploration. We explored the feasibility to conduct a WBE Indonesia, one global epicenters COVID-19 pandemic middle 2021, with fourth largest population world sewer non-sewered sewage systems are implemented. The resource collect samples on weekly or fortnightly basis grab and/or passive sampling methods, well qualitative quantitative identification ribonucleic acid (RNA) using real-time RT-PCR (RT-qPCR) environmental were explored.We initiated routine wastewater at three predetermined districts Special Region Yogyakarta Province. Water collected from central treatment plants (WWTPs), including manholes flowing WWTP, additional soil near source tracking (NST) locations (i.e., public spaces people congregate).We began collecting Delta wave Indonesia July 2021. From 10-week period, 54% (296/544) positive RNA. sample positivity rate decreased proportion reported incidence clinical cases community. highest 77% week 1, was obtained 2021 25% 10 by end September 2021.A feasible monitor burden infections. Future studies potential EWS signaling outbreaks transmissions this setting required.

Language: Английский

Citations

21
Quantification of SARS-CoV-2 in wastewater samples from hospitals treating COVID-19 patients during the first wave of the pandemic in Brazil DOI
Juliana Calábria de Araújo, Camila Leite Madeira, Thiago Bressani Ribeiro

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 860, P. 160498 - 160498

Published: Nov. 25, 2022

Language: Английский

Citations

21
Model training periods impact estimation of COVID-19 incidence from wastewater viral loads DOI Creative Commons
Maria L. Daza–Torres, J. Cricelio Montesinos-López, Minji Kim

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 858, P. 159680 - 159680

Published: Oct. 26, 2022

Wastewater-based epidemiology (WBE) has been deployed broadly as an early warning tool for emerging COVID-19 outbreaks. WBE can inform targeted interventions and identify communities with high transmission, enabling quick effective responses. As the wastewater (WW) becomes increasingly important indicator more robust methods metrics are needed to guide public health decision-making. This research aimed develop implement a mathematical framework infer incident cases of from SARS-CoV-2 levels measured in WW. We propose classification scheme assess adequacy model training periods based on clinical testing rates sensitivity predictions periods. A period is classified adequate when rate change greater than cases. present Bayesian deconvolution linear regression estimate WW data. The reproductive number estimated reconstructed using proposed modeling was applied three Northern California served by distinct treatment plants. results showed that essential provide accurate projections incidence.

Language: Английский

Citations

20
Wastewater-Based Surveillance of Mpox (Monkeypox): An Early Surveillance Tool for Detecting Hotspots DOI Creative Commons
Md. Aminul Islam,

Rakesh Kumar,

Prabhakar Sharma

et al.

Current Pollution Reports, Journal Year: 2024, Volume and Issue: 10(2), P. 312 - 325

Published: May 21, 2024

Abstract Purpose of Review This study aims to review recent literature reporting wastewater-based surveillance (WBS) the monkeypox virus (MPXV) with a major focus on sample collection, processing, and public health safety issues. Recent Findings Various studies revealed presence MPXV genetic markers in sewage, wastewater treatment plants, drainage systems hospitals multiple countries. Summary Mpox (formally monkeypox) has been identified more than hundred countries, confirming at least 93,937 cases from 06 May 2022 03 February 2024. The MPXV, causative agent for mpox, is zoonotic having genome DNA. Major symptoms disease are rash, fever, headache, chills lymph pain. It about 10% case fatality rate, without any approved vaccine, but researchers investigating development therapeutic vaccines. DNA signature long reported urine, stool, saliva, semen, spit samples clinical patients, make feasible tracking wastewater. Furthermore, have environmental samples. As possible hosts (e.g., small mammals rodents), their detection might indicate circulation including non-human hosts. persistence infective particles ambient environment not fully investigated. Also, risks transmission through contaminated milieu unknown. WBS can be an effective tool identifying hotspots trends communities. Graphical

Language: Английский

Citations

4
Wastewater-Based Surveillance of Antibiotic Resistance Genes Associated with Tuberculosis Treatment Regimen in KwaZulu Natal, South Africa DOI Creative Commons
Hlengiwe N. Mtetwa, Isaac Dennis Amoah, Sheena Kumari

et al.

Antibiotics, Journal Year: 2021, Volume and Issue: 10(11), P. 1362 - 1362

Published: Nov. 8, 2021

Essential components of public health include strengthening the surveillance infectious diseases and developing early detection prevention policies. This is particularly important for drug-resistant tuberculosis (DR-TB), which can be explored by using wastewater-based surveillance. study aimed to use molecular techniques determine occurrence concentration antibiotic-resistance genes (ARGs) associated with (TB) resistance in untreated treated wastewater. Raw/untreated (post-chlorination) wastewater samples were taken from three treatment plants (WWTPs) South Africa. The ARGs selected target drugs used first- second-line TB treatment. Both conventional polymerase chain reaction (PCR) more advanced droplet digital PCR (ddPCR) evaluated as strategies distribution ARGs. most abundant ARG was rrs gene, aminoglycosides, specifically streptomycin, median ranges 4.69-5.19 log copies/mL. In contrast, pncA drug pyrazinamide, least detected (1.59 2.27 copies/mL). Resistance bedaquiline detected, a significant finding because this new introduced Africa multi-drug resistant TB. study, therefore, establishes potential monitoring antibiotic communities.

Language: Английский

Citations

24